Skip to main content
Log in

Trans fatty acids in Canadian margarines: Recent trends

  • Article
  • Published:
Journal of the American Oil Chemists' Society

Abstract

The fatty acid composition and the trans fatty acid content of the top-selling 109 Canadian margarines were determined by a combined capillary gas-liquid chromatography/infrared spectroscopy method. The 109 brands accounted for 68% of the margarine brands sold in Canada and represented 74% of the market share. The mean level of total trans content in tub margarines (n=79) was 18.8% (g/100 g fatty acids) and ranged from 0.9 to 46.4%. The most frequent occurrence of trans in tub margarines was in the 15–20% range; 48 of the 79 tub brands were in this range but seven brands contained more than 40% trans. The trans content of hard margarines (n=30) ranged from 16.3 to 43.7% and the mean value was 34.3%. In 20 of the 109 brands, the levels of trans,trans isomers of linoleic acid exceeded the maximum level of 1% recommended for Canadian margarines. The levels of cis,trans/trans,cis isomers of linoleic acid were also high; 78 brands contained more than 1% and in 16 brands, the levels were in the 6–7% range. Linoleic acid content in the 109 brands ranged from 1.0 to 45.2% and averaged 18.3%. In 33 samples, linoleic acid was below the level of 5% recommended by an ad hoc committee of Health Canada. Moreover, in these, the total trans content exceeded 30%, and trans polyunsaturated fatty acid level was greater than 5%. There were eight margarines prepared from nonhydrogenated fat and their total trans content was below 2.5%. From the trans content and market share of each of the margarine brands, the average intake of trans fatty acids from margarine was estimated as 0.96 g/person/d. The intake of trans fatty acids in Canada from various sources was previously estimated by us as 8.4 g/person/d. Thus it is suggested that only 11% of the dietary trans fatty acids are supplied by margarines and the majority of trans fatty acids in the Canadian diet is derived from hidden fats in fast foods and bakery products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mensink, R.P., and M.B. Katan, Effect of Dietary trans Fatty Acids on High-Density and Low-Density Lipoprotein Cholesterol Levels in Healthy Subjects, N. Eng. J. Med. 323:439–445 (1990).

    Article  CAS  Google Scholar 

  2. Zock, P.L., and M.B. Katan, Hydrogenation Alternatives: Effects of trans Fatty Acids and Stearic Acid Versus Linoleic Acid on Serum Lipids and Lipoproteins in Humans, J. Lipid Res. 33:399–410 (1992).

    PubMed  CAS  Google Scholar 

  3. Mensink, R.P., P.L. Zock, M.B. Katan, and G. Hornstra, Effect of Dietary cis and trans Fatty Acids on Serum Lipoproteins Levels in Humans,:1493–1501 (1992).

    PubMed  CAS  Google Scholar 

  4. Nestel, P., M. Noakes, B. Belling, R. McArthur, P. Clifton, E. Janus, and M. Abbey, Plasma Lipoprotein Lipid and Lp(a) Changes with Substitution of Elaidic Acid for Oleic Acid in the Diet,:1029–1036 (1992).

    PubMed  CAS  Google Scholar 

  5. Lichtenstein, A.H., L.M. Ausman, W. Carrasco, J.L. Jenner, J.M. Ordovas, and E.J. Schaefer, Hydrogenation Impairs the Hydrogenation Effect of Corn Oil in Humans. Hydrogenation, trans Fatty Acids, and Plasma Lipids, Arterioscler. Thromb. 13:154–161 (1993).

    PubMed  CAS  Google Scholar 

  6. Judd, J.T., A. Clevidence, R.A. Muesing, J. Wittes, M.E. Sunkin, and J.J. Podczasy, Dietary trans Fatty Acids: Effects on Plasma Lipids and Lipoproteins on Healthy Men and Women, Am. J. Clin. Nutr. 59:861–868 (1994).

    PubMed  CAS  Google Scholar 

  7. Almendingen, K., O. Jordal, P. Kierulf, B. Sandstad, and J.I. Pedersen, Effects of Partially Hydrogenated Fish Oil, Partially Hydrogenated Soybean Oil and Butter on Serum Lipoproteins and Lp(a) in Men, J. Lipid Res. 36:1370–1384 (1995).

    PubMed  CAS  Google Scholar 

  8. Koletzko, B., Trans Fatty Acids may Impair Biosynthesis of Long-Chain Polyunsaturates and Growth in Man, Acta Paediatr. 81:302–306 (1992).

    PubMed  CAS  Google Scholar 

  9. Decsi, T., and B. Koletzko, Do trans Fatty Acids Impair Linoleic Acid Metabolism in Children? Ann. Nutr. Metab. 39:36–41 (1995).

    Article  PubMed  CAS  Google Scholar 

  10. Houwelingen, A.C.V., and G. Hornstra, Trans Fatty Acids in Early Human Development, World Rev. Nutr. Diet 75:175–178 (1994).

    Google Scholar 

  11. Ayagari, A., J.M. Peepies, and S.E. Carlson, Relationship of Isomeric Fatty Acids in Human Cord Blood to N3 and N6 Status, Pediatr. Res. 39:304A (abstract) (1996).

  12. Chen, Z.Y., G. Pelletier, R. Hollywood, and W.M.N. Ratnayake, Trans Fatty Acid Isomers in Canadian Human Milk, Lipids 30:15–21 (1995).

    Article  PubMed  CAS  Google Scholar 

  13. Ratnayake, W.M.N., and Z.Y. Chen, Trans Fatty Acids in Canadian Breast Milk and Diet, in Development and Processing of Vegetable Oils for Human Nutrition, edited by R. Przybylski and B.E. McDonald, AOCS Press, Champaign, 1995, pp. 20–35.

    Google Scholar 

  14. Ratnayake, W.M.N., and Z.Y. Chen, Trans, n-3 and n-6 Fatty Acids in Canadian Human Milk, Lipids 31:S-279–S-282 (1996).

    Article  CAS  Google Scholar 

  15. Beare, J.L., D. Tovel, and T.K. Murray, The Total cis Methylene Interrupted Fatty Acids in Canadian Margarines, Can. Med. Assoc. J. 93:1219 (1965).

    PubMed  CAS  Google Scholar 

  16. Beare-Rogers, J.L., L.M. Gray, and R. Hollywood, The Linoleic Acid and trans Fatty Acids of Margarines, Am. J. Clin. Nutr. 32:1805–1809 (1979).

    PubMed  CAS  Google Scholar 

  17. Sahasrabudhe, M.R., and C.J. Kurian, Fatty Acid Composition of Margarines in Canada, Can. Inst. Food Sci. Technol. J. 12:140–146 (1979).

    CAS  Google Scholar 

  18. Beare-Rogers, J.L., R. Hollywood, and E. O’Grady, Fatty Acids in Canadian Margarines, Can. J. Pub. Health 76:276–277 (1985).

    CAS  Google Scholar 

  19. Ratnayake, W.M.N., R. Hollywood, and E. O’Grady, Fatty Acids in Canadian Margarines, Can. Inst. Sci. Technol. J. 24:81–86 (1991).

    CAS  Google Scholar 

  20. Ratnayake, W.M.N., and G. Pelletier, Positional and Geometrical Isomers of Linoleic Acid in Partially Hydrogenated Oils, J. Am. Oil Chem. Soc. 69:95–105 (1992).

    Article  CAS  Google Scholar 

  21. Ratnayake, W.M.N. Determination of trans Unsaturation by Infrared Spectrometry and Determination of Fatty Acid Composition of Partially Hydrogenated Vegetable Oils and Animal Fats by Gas Chromatography/Infrared Spectrometry: Collaborative Study, J. Assoc. Off. Anal. Chem. Internation. 78:783–802 (1995).

    CAS  Google Scholar 

  22. Official Methods of Analysis of the Association of Official Analytical Chemists International, 16th ed., edited by P. Cunniff, (March Supplement), AOCS International, Arlington, 1995, Official Method 994.15, p. 26.

  23. Ministry of Supply and Services of Canada, Report of the Ad Hoc Committee on the Composition of Special Margarines, Cat. No. H44-46/1980E, 1980.

  24. Ministry of Supply and Services of Canada, Nutrition Recommendations: the Report of the Scientific Review Committee Ottawa, Department of National Health and Welfare, 1990.

  25. Ovesen, L., T. Leth, and K. Hansen, Fatty Acid Composition of Danish Margarines and Shortenings, with Special Emphasis on trans Fatty Acids, Lipids 31:971–975 (1996).

    Article  PubMed  CAS  Google Scholar 

  26. Bayard, C.C., and R.L. Wolff, Trans-18:1 Acids in French Tub Margarines and Shortenings: Recent Trends, J. Am. Oil Chem. Soc. 72:1485–1489 (1995).

    Article  CAS  Google Scholar 

  27. Mansour, M.P., and A.J. Sinclair, The trans Fatty Acid and Positional (sn-2) Fatty Acid Composition of Some Australian Margarines, Dairy Blends and Animal Fats, Asia Pacific J. Clin. Nutr. 3:155–163 (1993).

    Google Scholar 

  28. Kafatos, A., D. Chrysafidis, and E. Peraki, Fatty Acid Composition of Greek Margarines. Margarine Consumption by the Population of Crete and Its Relationship to Adipose Tissue Analysis, Int. J. Food Sci. Nutr. 45:107–114 (1994).

    CAS  Google Scholar 

  29. Lake, R., B. Thomson, G. Devane, and P. Scholes, Trans Fatty Acid Content of Selected New Zealand Foods, J. Food Comp. Anal. 9:365–374 (1996).

    Article  CAS  Google Scholar 

  30. Ulberth, F., and M. Henninger, Simplified Method for the Determination of trans Monoenes in Edible Fats by TLC-FID, J. Am. Oil Chem. Soc. 69:829–831 (1992).

    Article  CAS  Google Scholar 

  31. Emken, E.A., Trans Fatty Acids and Coronary Heart Disease Risk: Physicochemical Properties, Intake and Metabolism, Am. J. Clin. Nutr. 62:659S-669S (1995).

    Google Scholar 

  32. Lands, W.E.M., Biochemistry and Physiology of n-3 Fatty Acids, FASEB J, 6:2530–2536 (1992).

    PubMed  CAS  Google Scholar 

  33. Simopoulos, A.P., and J. Robinson, The Omega Plan, Harper-Collins, New York, 1988, pp. 24–36.

    Google Scholar 

  34. Privett, O.S., F. Phillips, H. Shimasaki, T. Nozawa, and E.C. Nickell, Studies of Effects of trans Fatty Acids in the Diet on Lipid Metabolism in Essential Fatty Acid Deficient Rats, Am. J. Clin. Nutr. 30:1009–1015 (1977).

    PubMed  CAS  Google Scholar 

  35. Hwang, D.H., and J.E. Kinsella, The Effects of trans,trans-Methyl Linoleate on the Concentration of Prostaglandins and Their Precursors in Rats, Prostaglandins 17:543–559 (1979).

    Article  PubMed  CAS  Google Scholar 

  36. Hill, E.G., S.B. Johnson, L.D. Lawson, M.M. Mahfouz, and R.T. Holman, Perturbation of Essential Fatty Acids by Dietary Partially Hydrogenated Vegetable Oil, Proc. Natl. Acad. Sci. USA 79:953–957 (1982).

    Article  PubMed  CAS  ADS  Google Scholar 

  37. Mahfouz, M.M., S. Johnson, and R.T. Holman, The Effect of Isomeric trans-18:1 Acids on the Desaturation of Palmitic, Linoleic and Eicosa-8,11,14-trienoic Acids by Rat Liver Microsomes, Lipids 19:214–222 (1980).

    Article  Google Scholar 

  38. Lawson, L.D., E.G. Hill, and R.T. Holman, Suppression of Arachidonic Acid in Lipids of Rat Tissues by Dietary Mixed Isomeric cis and trans Octadecenoates, J. Nutr. 113:1827–1835 (1983).

    PubMed  CAS  Google Scholar 

  39. Beyers, E.C., and E.A. Emken, Metabolites of cis,trans and trans,cis Isomers of Linoleic Acid in Mice and Incorporation into Tissue Lipids, Biochim. Biophys. Acta 1082:275–184 (1991).

    PubMed  CAS  Google Scholar 

  40. Holman, R.T., F. Pusch, B. Svigen, and H.J. Dutton, Unusual Isomeric Polyunsaturated Fatty Acids in Liver Phospholipids of Rats Fed Hydrogenated Oil, Proc. Natl. Acad. Sci. USA 88:4830–4834 (1991).

    Article  PubMed  CAS  ADS  Google Scholar 

  41. Ratnayake, W.M.N., Z.Y. Chen, G. Pelletier, and D. Weber, Occurrence of 5c,8c,11c,15t-Eicosatetraenoic Acid and Other Unusual Polyunsaturated Fatty Acids in Rats Fed Partially Hydrogenated Canola Oil, Lipids 29:707–714 (1994).

    Article  PubMed  CAS  Google Scholar 

  42. Ackman, R.G., S.N. Hooper, and D.L. Hooper, Linolenic Acid Artifacts from the Deodorization of Oils, J. Am. Oil Chem. 51:42–49 (1974)

    CAS  Google Scholar 

  43. Grundy, S.M., Lipids and Cardiovascular Disease, in Nutrition and Disease Update. Heart Disease, edited by D. Kritchevsky and K.K. Carrol, AOCS Press, Champaign, 1994, pp. 211–246.

    Google Scholar 

  44. Developing Brain and Behaviour: The Role of Lipids in Infant Formula, edited by J. Dobbins, Academic Press, San Diego, 1997, 537 pages.

  45. Domestic Production of Deodorized Oils and Fats, Catalogue 32006, Statistics Canada, Ottawa, Canada, 1996, Vol. 46, No. 12, p. 8.

  46. Ratnayake, W.M.N., R. Hollywood, E. O’Grady, and G. Pelletier, Fatty Acids in Some Common Food Items in Canada, J. Am. Coll. Nutr. 12:651–660 (1993).

    PubMed  CAS  Google Scholar 

  47. National Institute of Nutrition, Report on Tracking Nutrition Trends 1989–1994–1997, Canadian Facts, Toronto, Ontario, Canada, April 1997, pp. 27 and 28.

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to W. M. N. Ratnayake.

About this article

Cite this article

Ratnayake, W.M.N., Pelletier, G., Hollywood, R. et al. Trans fatty acids in Canadian margarines: Recent trends. J Amer Oil Chem Soc 75, 1587–1594 (1998). https://doi.org/10.1007/s11746-998-0098-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11746-998-0098-4

Key Words

Navigation