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Effect of pasture vs. concentrate diet on CLA isomer distribution in different tissue lipids of beef cattle

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Lipids

Abstract

This study examined the effects of feeding pasture vs. concentrate on the distribution of CLA isomers in the lipids of longissimus and semitendinosus muscle, liver and heart muscle, and subcutaneous fat in beef bulls. Sixty-four German Holstein and German Simmental bulls were randomly allocated to either an indoor concentrate system or periods of pasture feeding followed by a finishing period on a concentrate containing linseed to enhance their beef content of n−3 PUFA and CLA. The concentrations of CLA isomers in the different tissues were determined by GC and silver ion HPLC. The diet affected the distribution of individual CLA isomers in the lipids of the different tissues. The concentration (mg/100 g fresh tissue) of the most prominent isomer, cis-9,trans-11 18∶2, was increased up to 1.5 times in liver and heart tissue of bulls fed on pasture as compared with concentrate. However, no diet effect was observed for cis-9,trans-11 18∶2 in the lipids of longissimus muscle and subcutaneous fat. In all tissues, the second-most abundant CLA isomer in concentratefed bulls was trans-7,cis-9 18∶2. In contrast, trans-11,cis-13 18∶2 was the second-most abundant CLA isomer in all investigated tissue lipids of pasture-fed bulls. The concentration of the trans-11,cis-13 18∶2 isomer was up to 15 times higher in tissues of pasture-fed bulls as compared with concentrate-fed animals. Furthermone, diet affected the concentrations of the CLA trans,trans 18∶2 isomers. Pasture feeding significantly increased the concentrations of some trans,trans 18∶2 isomers as compared with concentrate, predominantly trans-12,trans-14 18∶2 and trans-11,trans-13 18∶2. Overall, pasture feeding resulted in significantly increased concentrations of the sum of CLA isomers in the lipids of longissimus, muscle, subcutaneous fat, heart and liver muscle of German Holstein and German Simmental bulls, but not in semitendinosus muscle.

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Abbreviations

Ag+-HPLC:

silver ion HPLC

LSM:

least square mean

ME:

methylester

SEM:

standard error of LSM

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Authors and Affiliations

  1. Department of Muscle Biology, Research Insitute for the Biology of Farm Animals, W.-Stahl-Allee 2, D-18196, Dummerstorf, Germany

    Dirk Dannenberger, Karin Nuernberg & Klaus Ender

  2. Department of Ceneitics and Biometry, Research Institute for the Biology of Farm Animals, W.-Stahl-Allee 2, D-18196, Dummerstorf, Germany

    Gerd Nuernberg

  3. Institute of Grassland and Environmental Research, SY23 3EB, Aberystwyth, UK

    Nigel Scollan

  4. Institute of Biochemistry and Food Chemistry, Department of Food Chemistry, University of Hamburg, D-20146, Hamburg, Germany

    Hans Steinhart

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  1. Dirk Dannenberger
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  2. Karin Nuernberg
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  3. Gerd Nuernberg
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  4. Nigel Scollan
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  5. Hans Steinhart
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  6. Klaus Ender
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Correspondence to Karin Nuernberg.

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Dannenberger, D., Nuernberg, K., Nuernberg, G. et al. Effect of pasture vs. concentrate diet on CLA isomer distribution in different tissue lipids of beef cattle. Lipids 40, 589–598 (2005). https://doi.org/10.1007/s11745-005-1420-2

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  • DOI: https://doi.org/10.1007/s11745-005-1420-2

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