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Blood stains of the Turin Shroud 2015: beyond personal hopes and limitations of techniques

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Abstract

In the early ‘80s, evidence was provided that, rather than a dye (red okra), hemoglobin was indeed responsible for the alleged blood stains of the Turin Shroud. Such stains were shown to belong to an MNS positive individual of the AB group, and the halos surrounding the blood stains were compatible with serum containing trace amounts of bilirubin, albumin and immunoglobulins. However, being only based on indirect and circumstantial evidence, most of these data were challenged. In the late ‘90s, together with the evidence of the gene coding β-globin, contamination between male and female DNA was documented on the Turin Shroud. Although the presence of male was more noticeable than female DNA, these data were considered null and void. These days, to establish that blood indisputably belongs to an MNS positive individual of the AB group, and to exclude DNA contamination, high-specificity techniques with monoclonal antibodies and molecular studies on nuclear and mitochondrial DNA are needed. Indeed, consistent with DNA contamination on the Turin Shroud, sequences from multiple subjects of different ethnic origins have been recently detected on the human mitochondrial genome extracted from dust particles of the linen. Innovative concepts are likely to come up using modern research approaches to evaluate the issue of blood stains of the Turin Shroud. Nor can we rule out the possibility that religious implications of the new findings on the Turin Shroud might be envisaged. Conceivably enough, the ongoing debate will be fierce and passionate, especially in the media.

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References

  1. Jumper EJ, Adler AD, Jackson JP, Pellicori SF, Heller JH, Druzik JR (1984) A comprehensive examination of the various stains and images on the Shroud of Turin. In: Lambert JB (Ed) Archaeological Chemistry III, ACS advances in chemistry 205, chapter 22. American Chemical Society, Washington DC: pp 447–476

  2. Adler A (2002) The Orphaned manuscript, a gathering of publications on the Shroud of Turin. In: Crispino D (ed) A Shroud Spectrum International Special Issue, 1st edn. Effatà Editrice, Turin, Italy, pp 1–147

  3. Antonacci M (2000) The Resurrection of the Shroud. M. Evans & Company, New York, p 328

    Google Scholar 

  4. Poulle E (2009) Les sources de l’histoire du linceul de Turin. Revue critique. Rev d’Histoire Ecclés 3–4:747–781

    Article  Google Scholar 

  5. Meacham W (1983) The authentication of the Turin Shroud, an issue in archeological epistemology. Curr Anthropol 24:283–295

    Article  Google Scholar 

  6. Pomilio M (1975) Il quinto Evangelio, Rusconi Ed, Milano

  7. Schwalbe LA, Rogers RN (1982) Physics and chemistry of the Shroud of Turin. A summary of the 1978 investigation. Anal Chim Acta 135:3–49

    Article  CAS  Google Scholar 

  8. McCrone WC (1982) Shroud image is the work of an artist. Skept Inq 6(3):35–36

    Google Scholar 

  9. McCrone WC, Skirius C (1980) Light Microscopical Study of the Turin Shroud I. Microsc 28(3–4):105–113

    Google Scholar 

  10. McCrone WC (1980) Light microscopical study of the Turin Shroud II. Microsc 28:115–128

    Google Scholar 

  11. McCrone WC (1981) Microscopical Study of the Turin ‘Shroud’ III. Microsc 29(1):19–39

    CAS  Google Scholar 

  12. McCrone WC (1990) The Shroud of Turin: blood or artist’s pigment? Acc Chem Res 23:77–83

    Article  CAS  Google Scholar 

  13. Heller JH, Adler AD (1980) Blood on the Shroud of Turin. Appl Opt 19(16):2742–2744

    Article  CAS  PubMed  Google Scholar 

  14. Heller JH, Adler A (1981) A chemical investigation of the Shroud of Turin. Can Forensic Soc Sci J 14:81–103

    Article  CAS  Google Scholar 

  15. Heller JH (1983) Report on the Shroud of Turin. Houghton-Mifflin Co, Boston

    Google Scholar 

  16. Pellicori SF (1980) Spectral properties of the Shroud of Turin. Appl Opt 19(12):1913–1920

    Article  CAS  PubMed  Google Scholar 

  17. Adler AD (1987) The origin and nature of blood on the Turin Shroud. Turin Shroud—image of christ?. Cosmos Printing Press Ltd., Hong Kong, pp 57–59

    Google Scholar 

  18. Gove HE (1996) Relic, Icon or Hoax? Carbon dating the Turin Shroud, 1st edn. CRC Press, pp 1–336

  19. Murphy K (2012) Janeway’s immunobiology, VIII edn. Garland Science Publishing, New York

    Google Scholar 

  20. King MC, Wilson AC (1975) Evolution at two levels in humans and chimpanzees. Science 188:107–116

    Article  CAS  PubMed  Google Scholar 

  21. Baima Bollone PL, Jorio M, Massaro ML (1981) La Dimostrazione della presenza di tracce di sangue umano sulla Sindone. Sindon 30:5–8

    Google Scholar 

  22. Baima Bollone, PL (2000) The forensic characteristics of the blood marks. In: The Turin Shroud: past, present, and future, ed. S. Scannerini and P. Savarino [Int. Scientific Symposium, Torino, Mar. 2-5, 2000]. Effata Editrice, Torino, Italy 125–135

  23. Black CM, McDougal JS, Evatt BL, Reimer CB (1991) Human markers for IgG2 and IgG4 appear to be on the same molecule in the chimpanzee. Immunology 72:94–98

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Wilson I (1998) The blood and the Shroud: new evidence that the world’s most sacred relic is real. Touchstone Books, New York

  25. Kohlbeck JA, Nitowski EI (1986) New evidence may explain image on Shroud of Turin. Biblic Archeol Rev 12:23–24

    Google Scholar 

  26. McCrone WC (1993) The scanning electron microscope (SEM) supplemented by the polarized light microscope (PLM), and vice versa. Scanning Microsc 7:1–4

    CAS  Google Scholar 

  27. Garlaschelli L (2010) Life-size reproduction of the Shroud of Turin and its image. J Imaging Sci Technol 54(4):40301-1–40301-14

    Article  Google Scholar 

  28. Schafersman SD (1982) Science, the public, and the Shroud of Turin –. Skept Inq 6(3):37–56

    Google Scholar 

  29. Riggi G (1991) The dusts of the Holy Shroud of Turin. New London, Connecticut, pp 1–11

  30. Moscardi G (2008) Analysis by Raman Microscopy of Powder Samples Drawn from the Turin Shroud. In: The Shroud of Turin: Perspectives on a multifaceted enigma. In: Proceedings of the Ohio State University Shroud Conference, Shroud Science Group International Conference, August 14–17, 2008 Ohio State University The Blackwell Hotel Columbus, Ohio (poster)

  31. Garza-Valdes L (1999) The DNA of God?. Doubleday, New York

    Google Scholar 

  32. Wilson I, Schwortz B (2000) The Turin Shroud: the illustrated evidence. Michael O’Mara Books, London, p 77

    Google Scholar 

  33. Baima Bollone PL, Jorio M, Massaro AL (1983) Identification of the Group of Traces of human blood on the Shroud. Shroud Spectr Int 6:3–6

    Google Scholar 

  34. Baima Bollone PL, Gaglio A (1984) Demonstration of blood, aloes and myrrh on the Holy Shroud with immunofluorescence techniques. Shroud Spectr Int 13:3–8

    Google Scholar 

  35. Baima Bollone PL, Gaglio A (1984) Ulteriori ricerche sul gruppo delle tracce di sangue umano sulla Sindone”. Sindon 33:9–14

    Google Scholar 

  36. Baima Bollone PL (1985) Gaglio A (1985) Ricerca degli antigeni M, N ed S nelle tracce di sangue sulla Sindone”. Sindon 34:9–13

    Google Scholar 

  37. Rearden A, Magnet A, Kudo S, Fukuda M (1993) Glycophorin B and glycophorin E genes arose from the glycophorin A ancestral Gene via two duplications during primate evolution. J Biol Chem 268:2260–2267

    CAS  PubMed  Google Scholar 

  38. Patnaik SK, Helmberg W, Blumenfeld OO (2014) BGMUT Database of Allelic Variants of Genes Encoding Human Blood Group Antigens. Trans Med Hemother 41(5):346–351

    Google Scholar 

  39. Ellis L (1999) Paleoserology. In: Archaeological method and theory: an encyclopedia, Garland Publishing, Inc. A Member of the Taylor and Francis Group New York and London, pp 1–707

  40. Yamamoto F (2004) Review: ABO blood group system-ABH oligosaccharide antigens, anti-A and anti-B, A and B glycosyltransferases, and ABO genes. Immunohematology 20:3–22

    CAS  PubMed  Google Scholar 

  41. Hooft P, van de Voorde H, Van Dijck P (1991) Blood-group simulating activity in aerobic gramnegative oral bacteria cultured from fresh corpses. Forensic Sci Int 50:263–268

    Article  CAS  PubMed  Google Scholar 

  42. Gove HE, Mattingly SJ, David AR, Garza-Valdes LA (1997) Problematic source of organic contamination of linen. Nucl Instrum Methods Phys Res B 123:504–507

    Article  CAS  Google Scholar 

  43. Crainic K, Durigon M, Oriol R (1989) ABO tissue antigens of Egyptian mummies”. Forensic Sci Int 43:113–124

    Article  CAS  PubMed  Google Scholar 

  44. Kłys M, Opolska-Bogusz B, Próchnicka B (1999) A serological and histological study of the Egyptian mummy “Iset Iri Hetes’ from the Ptolemaic period III-I B.C. Forensic Sci Int 99:229–233

    Article  PubMed  Google Scholar 

  45. Sokal RR, Lengyel IA, Derish PA, Wooten MC, Ode NL (1987) Spatial autocorrelation of ABO serotypes in Mediaeval cemeteries as an indicator of ethnic and familial structure. J Archaeol Sci 14:615–633

    Article  Google Scholar 

  46. Spalter SH, Kaveri SV, Bonnin E, Mani JC, Cartron JP, Kazatchkine MD (1999) Normal human serum contains natural antibodies reactive with autologous ABO blood group antigens. Blood 93:4418–4424

    CAS  PubMed  Google Scholar 

  47. van Oss CJ (2004) Natural versus Regular Antibodies. Protein J 23:357 (Letter to the Editor)

    Article  PubMed  Google Scholar 

  48. Kearse KP (2012) Blood on the Shroud of Turin: an immunological review. https://www.shroud.com/pdfs/kearse. Accessed 14 Mar 2016

  49. Porter DR (2005) How do you know that there is real blood on the Shroud? http://theshroudofturin.blogspot.com/201…blood.htm. Accessed date 6 Nov 2005

  50. Kearse KP (2013) Icons, science, and faith: comparative examination of the Shroud of Turin and the Sudarium of Oviedo. Theol Sci 11(1):52–61

    Article  Google Scholar 

  51. Independent Forensics (2011) Rapid Stain Identification of Human Blood (RSIDTM-Blood) Technical Information and Protocol Sheet for Universal Buffer, cat# 0330 RSID Blood Universal Buffer. http://www.galantos.eu. Accessed 14 Mar 2016

  52. Kimura A, Uda T, Nakashima S, Ikeda H, Yasuda S, Osawa M, Tsujiet T (1993) ABO blood grouping of bloodstains by sandwich ELISA using monoclonal antibody specific for human red cell band 3. Int J Leg Med 105:209–212

    Article  CAS  Google Scholar 

  53. Hummel S (2002) ABO blood group genotyping of ancient DNA by PCR-RFLP. Int J Legal Med 116:327–333

    PubMed  Google Scholar 

  54. Sano T, Smith CL, Cantor CR (1992) Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. Science 258:120–122

    Article  CAS  PubMed  Google Scholar 

  55. Torres JM, Borja C, Olivares EG (2002) Immunoglobulin G in 1.6 million-year-old fossil bones from Venta Micena (Granada, Spain). J Archaeol Sci 29:167–175

    Article  Google Scholar 

  56. Storry JR, Olsson ML (2009) The ABO blood group system revisited: a review and update. Immunohematology 25:48–59

    CAS  PubMed  Google Scholar 

  57. Ruangturakit S, Rojanasuphot S, Srijuggravanvong A, Duangchanda S, Nuangplee S, Igarashi A (1994) Storage stability of dengue IgM and IgG antibodies in whole blood and serum dried on paper filter strips detected by ELISA. Southeast Asian. J Trop Med Public Health 25:560–564

    CAS  Google Scholar 

  58. Kircher M, Kelso J (2010) High-throughput DNA sequencing-concepts and limitations. BioEssays 32:524–536

    Article  CAS  PubMed  Google Scholar 

  59. Prüfer K, Racimo F, Patterson N, Jay F, Sankararaman S, Sawyer S, Heinze A, Renaud G, Sudmant PH, de Filippo C, Li H, Mallick S, Dannemann M, Fu Q, Kircher M, Kuhlwilm M, Lachmann M, Meyer M, Ongyerth M, Siebauer M, Theunert C, Tandon A, Moorjani P, Pickrell J, Mullikin JC, Vohr SH, Green RE, Hellmann I, Johnson PL, Blanche H, Cann H, Kitzman JO, Shendure J, Eichler EE, Lein ES, Bakken TE, Golovanova LV, Doronichev VB, Shunkov MV, Derevianko AP, Viola B, Slatkin M, Reich D, Kelso J, Pääbo S (2014) The complete genome sequence of a Neanderthal from the Altai Mountains. Nature 505(7481):43–49

    Article  PubMed  PubMed Central  Google Scholar 

  60. Gupta PK (2008) Single-molecule DNA sequencing technologies for future genomics research. Trends Biotechnol 26(11):602–611

    Article  CAS  PubMed  Google Scholar 

  61. Melton T, Holland C, Holland M (2012) Forensic mitochondrial DNA analysis: current practice and future potential. Forensic Sci Rev 24:101–122

    CAS  PubMed  Google Scholar 

  62. Nickell J (1998) Inquest on the Shroud of Turin: latest scientific findings, Prometheus Books, pp 1–184

  63. Wilson J (1979) The Shroud of Turin: the Burial Cloth of Jesus Christ? Revised edition. Image Books; Publisher, pp: 320 (English translation of the Memorandum)

  64. Zugibe FT (1981) The Cross and the Shroud, a medical examiner investigates the crucifixion. McDonagh & Co., Cresskill, p 214

    Google Scholar 

  65. Damon PE, Donahue DJ, Gore BH, Hatheway AL, Jull AJT, Linick TW, Sercel PJ, Toolin LJ, Bronk CR, Hall ET, Hedges REM, Housley R, Law IA, Perry C, Bonani G, Trumbore S, Woelfli W, Ambers JC, Bowman SGE, Leese MN, Tite MS (1989) Radiocarbon dating of the Shroud of Turin. Nature 337(1989):611–615

    Article  CAS  Google Scholar 

  66. Riani M, Atkinson AC, Fanti G, Crosilla F (2013) Regression analysis with partially labelled regressors: carbon dating of the Shroud of Turin. Stat Comput 23:551–561

    Article  Google Scholar 

  67. Rogers RN (2005) Studies on the radiocarbon sample from the Shroud of Turin. Thermochim Acta 425:189–194

    Article  CAS  Google Scholar 

  68. Danin A, Whanger A, Baruch U, Whanger M (1999) Flora of the Shroud of Turin. Missouri Botanical Garden Press, St. Louis 52

    Google Scholar 

  69. Barcaccia G, Galla G, Achilli A, Olivieri A, Torroni A (2015) Uncovering the sources of DNA found on the Turin Shroud. Sci Rep 5:14484. doi:10.1038/srep14484

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  70. Lincoln A (2014) How babies were made in Jesus’ Time. Biblic Archaeol Rev 40(6):42–49

    Google Scholar 

  71. Antonucci M (2012) Particle radiation from the body could explain the Shroud’s image and its carbon dating. Sci Res Assays 7(29):2613–2623

    Google Scholar 

  72. Nickell J (1989) Unshrouding a mystery: science, pseudoscience, and the cloth of Turin. Skept Inq 13:296–299

    Google Scholar 

  73. Rodante S (1989) Formazione naturale delle impronte sindoniche: sudore di sangue, aloe e mirra. Typescript, Symposium Scientifique International de Paris sur le Linceul de Turin, September 7–8, pp. 1–9

  74. Fanti G, Moroni M (2002) Comparison of luminance between face of Turin Shroud man and experimental results. J Imaging Sci Technol 46(2):142–154

    CAS  Google Scholar 

  75. Pesce-Delfino V (1980) E l’uomo creò la Sindone, Dedalo Publisher, Bari, pp 304

  76. Allen NPL (1993) Is the Shroud of Turin the first recorded photograph? The South African Journal of Art History, November 11, 23–32

  77. Baldacchini G, Di Lazzaro P, Murra D, Fanti G (2008) Coloring linens with excimer lasers to simulate the body image of the Turin Shroud. Appl Opt 47(9):1278–1285

    Article  PubMed  Google Scholar 

  78. Di Lazzaro P, Murra D, Nichelatti E, Santoni A, Baldacchini G (2012) Superficial and Shroud-like coloration of linen by short laser pulses in the vacuum ultraviolet. Appl Opt 51:8567–8578

    Article  PubMed  Google Scholar 

  79. Fanti G (2011) Hypotheses regarding the formation of the body image on the Turin Shroud. A critical compendium. J Imaging Sci Technol 55:1–14

    Article  Google Scholar 

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Acknowledgments

The Authors wish to thank Drs. Waltr Memmolo, Antonio Salvio and Maria Benedetta Donati for helpful discussions.

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  1. Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli “Federico II”, Via S. Pansini 5, 80131, Naples, Italy

    Giovanni Di Minno, Rosanna Scala & Itala Ventre

  2. Dipartimento di Epidemiologia e Prevenzione, IRCCS Istituto Neurologico Mediterraneo, Pozzilli, Isernia, Italy

    Giovanni de Gaetano

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  1. Giovanni Di Minno
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Correspondence to Giovanni Di Minno.

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G.D.M. and G.d.G. have served on advisory boards unrelated to the topics discussed in the present commentary. R.S. has nothing to declare.

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Di Minno, G., Scala, R., Ventre, I. et al. Blood stains of the Turin Shroud 2015: beyond personal hopes and limitations of techniques. Intern Emerg Med 11, 507–516 (2016). https://doi.org/10.1007/s11739-016-1433-7

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