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Inhibitory effects of Carpinus tschonoskii leaves extract on CpG-stimulated pro-inflammatory cytokine production in murine bone marrow-derived macrophages and dendritic cells

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Abstract

This report describes the anti-inflammatory effects of MeOH extract from leaves of Carpinus tschonoskii (CE) on primary bone marrow-derived macrophage (BMDMs) and dendritic cells (BMDCs). Primary BMDMs and BMDCs were used for pro-inflammatory cytokine production and Western blot analysis. Human embryonic kidney cell line 293 T (HEK293 T) was used to access NF-κB activity. In all cases, CpG DNA was used to stimulate the cells. The CE (0–150 μg/ml) was treated to BMDMs, BMDCs, and HEK293T cells. CE pre-treatment in CpG-stimulated BMDMs and BMDCs showed a dose-dependent inhibitory effect on pro-inflammatory cytokine (e.g., IL-12 p40, IL-6, and TNF-α) production as compared to non-treated controls. The CE pre-treatment had no significant inhibition on mitogen-activated protein kinases (MAPKs) phosphorylation but strongly inhibited IκBα degradation. In NF-κB reporter gene assay, the CE pre-treatment inhibited NF-κB-dependent luciferase activity in a dose-dependent manner. Taken together, these data suggest that CE has significant inhibitory effect on pro-inflammatory cytokine production and warrant further studies concerning potentials of CE for medicinal uses.

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Acknowledgment

This study was supported by a grant from the National R&D Program for Cancer Control, Ministry of Health and Welfare, Republic of Korea (1120340).

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. School of Medicine, Jeju National University, Jeju, 690-756, South Korea

    Jung-Eun Koo, Hye-Jin Hong, Vivek Bhakta Mathema, Hee-Kyoung Kang, Jin-Won Hyun, Young-Ree Kim, Young-Hee Maeng, Chang-Lim Hyun, Weon-Young Chang & Young-Sang Koh

  2. Institute of Medical Science, Jeju National University, Jeju, 690-756, South Korea

    Jung-Eun Koo, Hye-Jin Hong, Vivek Bhakta Mathema, Hee-Kyoung Kang, Jin-Won Hyun, Young-Ree Kim, Young-Hee Maeng & Young-Sang Koh

  3. Department of Marine Life Sciences, Jeju National University, Jeju, 690-756, South Korea

    Gi-Young Kim

  4. Department of Microbiology and Immunology, School of Medicine, Jeju National University, 102 Jejudaehakno, Jeju, 690-756, South Korea

    Young-Sang Koh

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  1. Jung-Eun Koo
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  2. Hye-Jin Hong
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  9. Chang-Lim Hyun
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Correspondence to Young-Sang Koh.

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Koo, JE., Hong, HJ., Mathema, V.B. et al. Inhibitory effects of Carpinus tschonoskii leaves extract on CpG-stimulated pro-inflammatory cytokine production in murine bone marrow-derived macrophages and dendritic cells. In Vitro Cell.Dev.Biol.-Animal 48, 197–202 (2012). https://doi.org/10.1007/s11626-012-9495-y

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  • DOI: https://doi.org/10.1007/s11626-012-9495-y

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