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Isoflurane enhances the expression of cytochrome C by facilitation of NMDA receptor in developing rat hippocampal neurons in vitro

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Summary

This study examined the effects of clinically relevant concentrations of isoflurane on the amplitude of NMDA receptor current (INMDA) and the expression of cytochrome C in cultured developing rat hippocampal neurons. The hippocampi were dissected from newborn Sprague-Dawley rats. Hippocampal neurons were primarily cultured for 5 days and then treated with different concentrations of isoflurane [(0.25, 0.5, 0.75, 1 minimum alveolar concentration (MAC))]. The peak of INMDA was recorded by means of the whole cell patch clamp technique. The cytochrome C level was detected by Western blotting and quantitative real-time PCR. Our results showed that isoflurane (0.25, 0.5, 0.75 and 1 MAC) potentiated the amplitude of INMDA by (116±8.8)%, (122±11.7)%, (135±14.3)% and (132±14.6)%, respectively, and isoflurane increased the mRNA expression of cytochrome C in a concentration-dependent manner. The cytochrome C mRNA expression reached a maximum after 0.5 MAC isoflurane stimulation for 6 h (P<0.05). It was concluded that isoflurane enhances the expression of cytochrome C in cultured rat hippocampal neurons, which may be mediated by facilitation of NMDA receptor.

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Authors and Affiliations

  1. Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Yilin Zhao  (赵以林), Xiaogao Jin  (金小高), Jintao Wang  (王金韬), Lei Tan  (谭 蕾), Shiyong Li  (李世勇) & Ailin Luo  (罗爱林)

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  1. Yilin Zhao  (赵以林)
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  2. Xiaogao Jin  (金小高)
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  3. Jintao Wang  (王金韬)
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  4. Lei Tan  (谭 蕾)
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  5. Shiyong Li  (李世勇)
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  6. Ailin Luo  (罗爱林)
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Correspondence to Ailin Luo  (罗爱林).

Additional information

This work was supported by grants from the National Natural Science Foundation of China (No. 30772086; No. 30901390).

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Zhao, Y., Jin, X., Wang, J. et al. Isoflurane enhances the expression of cytochrome C by facilitation of NMDA receptor in developing rat hippocampal neurons in vitro . J. Huazhong Univ. Sci. Technol. [Med. Sci.] 31, 779–783 (2011). https://doi.org/10.1007/s11596-011-0676-9

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  • DOI: https://doi.org/10.1007/s11596-011-0676-9

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