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Application of Surface-Enhanced Raman Spectroscopy for Detection of Beta Amyloid Using Nanoshells

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Abstract

Currently, no methods exist for the definitive diagnosis of AD premortem. β-amyloid, the primary component of the senile plaques found in patients with this disease, is believed to play a role in its neurotoxicity. We are developing a nanoshell substrate, functionalized with sialic acid residues to mimic neuron cell surfaces, for the surface-enhanced Raman detection of β-amyloid. It is our hope that this sensing mechanism will be able to detect the toxic form of β-amyloid, with structural and concentration information, to aid in the diagnosis of AD and provide insight into the relationship between β-amyloid and disease progression. We have been successfully able to functionalize the nanoshells with the sialic acid residues to allow for the specific binding of β-amyloid to the substrate. We have also shown that a surface-enhanced Raman spectroscopy response using nanoshells is stable and concentration-dependent with detection into the picomolar range.

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Acknowledgements

The authors acknowledge the support of the National Institutes of Health (grant no. STTR-1R41AG025586-01 and grant no. R21-NS050346-01). The authors acknowledge the support of the Air Force (STTR FA9550-05-C-0019). Hope Beier acknowledges the support of a National Science Foundation Graduate Research Fellowship.

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Author notes

  1. James E. Henry

    Present address: Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA, USA

Authors and Affiliations

  1. Department of Biomedical Engineering, Texas A&M University, 337 Zachry Engineering Center, 3120 TAMU, College Station, TX, 77843-3120, USA

    Hope T. Beier, I-Hsien Chou, Melodie E. Benford & Gerard L. Coté

  2. Department of Chemical & Biochemical Engineering, University of Maryland Baltimore County, Baltimore, MD, USA

    Christopher B. Cowan, James Pallikal, James E. Henry & Theresa A. Good

  3. Nanospectra Biosciences, Inc., Houston, TX, USA

    Joseph B. Jackson

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  1. Hope T. Beier
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Correspondence to Hope T. Beier.

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Beier, H.T., Cowan, C.B., Chou, IH. et al. Application of Surface-Enhanced Raman Spectroscopy for Detection of Beta Amyloid Using Nanoshells. Plasmonics 2, 55–64 (2007). https://doi.org/10.1007/s11468-007-9027-x

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