Abstract
The imaging theory of Raman induced Kerr effect spectroscopy (RIKES) in nonlinear confocal microscopy is presented in this paper. Three-dimensional point spread function (3D-PSF) of RIKES nonlinear confocal microscopy in isotropic media is derived with Fourier imaging theory and RIKES theory. The impact of nonlinear property of RIKES on the spatial resolution and imaging properties of confocal microscopy have been analyzed in detail. It is proved that RIKES nonlinear confocal microscopy can simultaneously provide more information than two-photon confocal microscopy concerning molecular vibration mode, vibration orientation and optically induced molecular reorientation, etc. It is shown that RIKES nonlinear confocal microscopy significantly enhances the spatial resolution and imaging quality of confocal microscopy and achieves much higher resolution than that of two-photon confocal microscopy.
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Squier J, Muller M. High resolution nonlinear microscopy: A review of sources and methods for achieving optical imaging. Rev Sci Instrum, 2001, 72(7): 2855–2867
Helmchen F, Denk W. New developments in multiphoton microscopy. Curr Opin Neurobiol, 2002, 12: 593–601
Gauderon R, Lukins P B, Sheppard C J R. Simultaneous multichannal nonlinear imaging: Combined two-photon excited fluorescence and second-harmonic generation microscopy. Micron, 2001, 32: 685–689
Tang Z L, Yang C P, Pei H J, et al. Imaging theory and resolution improvement of two-photon confocal microscopy. Sci China Ser A, 2002, 45(11): 1468–1478
Tang Z L, Xing D, Liu S H, Imaging theory of nonlinear second harmonic and third harmonic generations in confocal microscopy, Sci China Ser G-Phys Mech Astron, 2004, 47(1): 8–16
Mertz J, Moreaux L. Second-harmonic generation by focused excitation of inhomogeneously distributed scatterers. Opt Commun, 2001, 196: 325–330
Yuan J H, Xiao F R, Cheng C F, et al. The intensity distribution of collected signals in coherent anti-Stokes Raman scattering microscopy. Colloid Surface A, 2005, 257–258: 525–534
Yakovlev V V. Broadband cost-effective nonlinear Raman microscopy. Proc SPIE, 2004, 5323: 214–220
Mertz J. Nonlinear microscopy: New techniques and applications. Curr Opin Neurobiol, 2004, 14: 610–616
Volkmer A. Vibrational imaging and microspectroscopies based on coherent anti-Stokes Raman scattering microscopy. J Phys D, 2005, 38: 59–81
Kobayashi N, Egami C. High-resolution optical storage by use of minute spheres. Opt Lett, 2005, 30(3): 299–301
Huff T B, Cheng J X. In vivo coherent anti-Stokes Raman scattering imaging of sciatic nerve tissue. J Microsc, 2007, 225(2): 175–182
Potma E O, de Boeij W P, Wiersma D A. Femtosecond dynamics of intracellular water probed with nonlinear optical Kerr effect microspectroscopy. Biophys J, 2001, 80(6): 3019–3024
Yasui T, Minoshima K, Abraham E, et al. Microscopic time-resolved two-dimensional imaging with a femtosecond amplifying optical Kerr gate. Appl Opt, 2002, 41(24): 5191–5194
Heiman D, Hellwarth R W, Levenson M D, et al. Raman-induced Kerr effect. Phys Rev Lett, 1976, 36(4): 189–192
Bhatia P S, Keto J W. Pressure and power dependence of the optically heterodyne Raman-induced Kerr effect line shape. Phys Rev A, 1999, 59(5): 4045–4051
Giraud G, Karolin J, Wynne K. Low-frequency modes of peptides and globular proteins in solution observed by ultrafast OHD-RIKES spectroscopy. Biophysics, 2003, 85: 1903–1913
Zhang N, Zhang D J, Zhang S C, et al. Characteristics and quantitative of negative ion in salt aqueous solution by Raman spectroscopy at −170°C. Sci China Ser D-Earth Sci, 2006, 49(2): 124–132
Xu Y M, Lu C Z. Raman spectroscopic study on structure of human immu-nodeficiency virus (HIV) and hypericin-induced photosen-sitive damage of HIV. Sci China Ser C-Life Sci, 2005, 48(2): 117–132
Eesley G L. Coherent Raman Spectroscopy. New York: Pergamon Press, 1981. 40–50
Yu Y Q, Zhou X G, Lin K, et al. Profile comparison between the Raman-induced Kerr effect spectrum and photoacoustic Raman spectrum of methane. Acta Phys Sin, 2006, 55(6): 2740–2745
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Supported by the Natural Science Foundation of Guangdong Province of China (Grant No. 05005926), the Plan Project of Science and Technology of Guangzhou City (Grant No. 2007J1-C0011) and Open Foundation of the Key Laboratory of Laser Life Science, Ministry of Education of China (2007–05)
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Guo, L., Tang, Z. & Xing, D. Theoretical investigation on Raman induced kerr effect spectroscopy in nonlinear confocal microscopy. Sci. China Ser. G-Phys. Mech. Astron. 51, 788–796 (2008). https://doi.org/10.1007/s11433-008-0086-6
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DOI: https://doi.org/10.1007/s11433-008-0086-6