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Anti-inflammatory and gastromucosal protective effects of Calotropis procera (Asclepiadaceae) stem bark

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Abstract

This study was aimed at evaluating the anti-inflammatory and gastromucosal protective effect of chloroform extract (CH) and hydroalcoholic extract (HE) of the stem bark of Calotropis procera obtained successively by cold maceration. The anti-inflammatory effect of the CH and HE extracts of the stem bark of Calotropis procera against carrageenan-induced paw oedema and also its gastromucosal protective effect has been studied by using two acute models: aspirin (100 mg/kg, p.o.) and ethanol (96%) in albino rats. CH and HE extracts showed significant anti-inflammatory activity at 200 and 400 mg/kg, while CH extract at 400 mg/kg was also found to have a significant gastromucosal protective effect. As part of investigations to obtain compounds with anti-inflammatory and gastromucosal protective effects in this work, a bioassay was carried out with fractions obtained from the CH extract with n-hexane (NF1), 1-butanol (BF1), ethyl acetate (EF1) and chloroform (CF1). The HE extract of the stem bark was fractionated with n-hexane (NF2), 1-butanol (BF2), ethyl acetate (EF2), chloroform (CF2) and water (WF2). The fractions were evaluated for their anti-inflammatory and gastromucosal protective effects. Fractions NF1, CF1, BF2 and EF2 (20 mg/kg) showed significant anti-inflammatory activity, while NF1 and BF2 (20 mg/kg) also showed gastromucosal protective effects. The results obtained for gastromucosal protective effects were also well supported by histopathological examination of the open excised rat stomach.

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Acknowledgments

The authors are thankful to the Principal, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, India for providing experimental facilities to carry out the work.

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Authors and Affiliations

  1. R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, 425405, Maharashtra, India

    Nagesh Tour

  2. Nashik Gramin Shikshan Prasarak Mandal’s College of Pharmacy, Anjaneri, Nashik, 422005, Maharashtra, India

    Gokul Talele

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  1. Nagesh Tour
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  2. Gokul Talele
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Correspondence to Nagesh Tour.

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Tour, N., Talele, G. Anti-inflammatory and gastromucosal protective effects of Calotropis procera (Asclepiadaceae) stem bark. J Nat Med 65, 598–605 (2011). https://doi.org/10.1007/s11418-011-0522-1

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  • DOI: https://doi.org/10.1007/s11418-011-0522-1

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