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Dietary oil modifies the plasma proteome during aging in the rat

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Abstract

Fatty acids and other components of the diet may modulate, among others, mechanisms involved in homeostasis, aging, and age-related diseases. Using a proteomic approach, we have studied how dietary oil affected plasma proteins in young (6 months) or old (24 months) rats fed lifelong with two experimental diets enriched in either sunflower or virgin olive oil. After the depletion of the most abundant proteins, levels of less abundant proteins were studied using two-dimensional electrophoresis and mass spectrometry. Our results showed that compared with the sunflower oil diet, the virgin olive oil diet induced significant decreases of plasma levels of acute phase proteins such as inter-alpha inhibitor H4P heavy chain (at 6 months), hemopexin precursor (at 6 and 24 months), preprohaptoglobin precursor (at 6 and 24 months), and α-2-HS glycoprotein (at 6 and 24 months); antioxidant proteins such as type II peroxiredoxin (at 24 months); proteins related with coagulation such as fibrinogen γ-chain precursor (at 24 months), T-kininogen 1 precursor (at 6 and 24 months), and apolipoprotein H (at 6 and 24 months); or with lipid metabolism and transport such as apolipoprotein E (at 6 and 24 months) and apolipoprotein A-IV (at 24 months). The same diet increased the levels of apolipoprotein A-1 (at 6 and 24 months), diminishing in general the changes that occurred with age. Our unbiased analysis reinforces the beneficial role of a diet rich in virgin olive oil compared with a diet rich in sunflower oil, modulating inflammation, homeostasis, oxidative stress, and cardiovascular risk during aging.

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Acknowledgments

The proteomic analysis was carried out in the UCO-SCAI proteomics facility, a member of ProteoRed network. This work was supported by a Proyectos Internacionales Grant from Junta de Andalucía to JMV, a Proyectos de Excelencia Grant (P09-CVI-4887) from Junta de Andalucía to JMV, BIO-276 Grants from Junta de Andalucía and the University of Córdoba to JMVM, and a Proyectos de Excelencia Grant (AGR-922) from Junta de Andalucía) to FPJ. MS was funded by AGR922 and BIO-276 Grants.

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  1. Departamento de Biología Celular, Fisiología e Inmunología, University of Córdoba, Campus Rabanales Ed. Severo Ochoa, 3a planta, 14014, Córdoba, Spain

    Mónica Santos-González & José M. Villalba

  2. Lipid and Atherosclerosis Unit, IMIBIC/Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain

    José López-Miranda & Francisco Pérez-Jiménez

  3. CIBER Fisiopatología Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Córdoba, Spain

    José López-Miranda & Francisco Pérez-Jiménez

  4. Centro Andaluz de Biología del Desarrollo (CABD), University Pablo de Olavide-CSIC, Seville, Spain

    Plácido Navas

  5. CIBER Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, Sevilla, Spain

    Plácido Navas

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  1. Mónica Santos-González
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  2. José López-Miranda
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  3. Francisco Pérez-Jiménez
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  4. Plácido Navas
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  5. José M. Villalba
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Correspondence to José M. Villalba.

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Santos-González, M., López-Miranda, J., Pérez-Jiménez, F. et al. Dietary oil modifies the plasma proteome during aging in the rat. AGE 34, 341–358 (2012). https://doi.org/10.1007/s11357-011-9239-z

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