Abstract
In this study, a novel 18-residue linear antimicrobial peptide derived from the central part of the bovine hemoglobin α-subunit was identified. The peptide was purified by a combination of cationic exchange and reversed-phase high-performance liquid chromatography. The sequence was determined to be VNFKLLSHSLLVTLASHL. The theoretical molecular weight of this peptide was calculated to be 1992.38 Da, which is the same as that determined (1992.401 Da) by matrix-assisted laser desorption ionization mass spectrometry. Sequence analysis showed that there is a high degree of homology in this peptide among hemoglobin α-subunits of bovine, sheep, deer, porcine, and human. In a radial-diffusion plate assay, this purified peptide exhibited antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans.
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References
Charlotte T, Liying C, Anderson RachelC (2005) Isolation and characterization of antimicrobial peptides from deer neutrophils. Int J Antimicrob Agents 26:165–169
Cornelia L, Susann B, Cornelia H (2003) Human hemoglobin-derived peptides exhibit antimicrobial activity: a class of host defense peptides. J Chromatogr B 791:345–356
Craig AP, Jiang H, Yoshi T (2001) Broad-spectrum antimicrobial activity of hemoglobin. Bioorg Med Chem 9:377–382
Fogaça AC, da Silva PI Jr, Miranda MT (1999) Antimicrobial activity of bovine hemoglobin fragment in the tick boophilus microplus. J Biol Chem 274:25330–25334
Machado A, Sforça ML, Miranda A (2007) Truncation of amidated fragment 33–61 of bovine alpha-hemoglobin: effects on the structure and anticandidal activity. Biopolymers 88:413–426
Maurcio LS, Alessandra M, Rita CR (2005) The micelle-bound structure of an antimicrobial peptide derived from the-chain of bovine hemoglobin isolated from the tick boophilus microplus. Biochemistry 44:6440–6451
Naïma NA, Véronique DD, Estelle YA (2008) Bovine hemoglobin: an attractive source of antibacterial peptides. Peptides 29:969–977
Nakajima Y, Ogihara K, Taylor D (2003) Antibacterial hemoglobin fragments from the midgut of the soft tick, Ornithodoros moubata (Acari: Argasidae). J Med Entomol 40:78–81
Paweł M, Kinga W, Jerzy S (2000) Antimicrobial peptides derived from heme-containing proteins: hemocidins. Antonie Van Leeuwenhoek 77:197–207
Pawel M, Kinga W, Lukasz W (2004) Antibacterial hemoglobin peptides in human menstrual blood. Peptides 25:1839–1847
Pawel M, Michal S, Jan PM (2007) HbB115–146 is an acidophilic antibacterial peptide potentiating the activity of human defensins, cathelicidin and lysozyme. Am J Reprod Immunol 57:81–91
Rachid D, Veronique D, Loredana B (2005) New antibacterial peptide derived from bovine hemoglobin. Peptides 26:713–719
Steinberg DA, Lehrer RI (1997) Designer assays for antimicrobial peptides. Disputing the ‘one-size-fits-all’ theory. Methods Mol Biol 78:169–186
Wang LL, Pan XL, Ma Y (2006) Antimicrobial peptide may originate from hemoglobin and play a role in the antibacterial mechanisms of endometrium (article in Chinese). Zhonghua Yi Xue Za Zhi 86:2044–2048
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This study was supported by the Science and Technology Innovation Foundation for Distinguished Young Scientists of Henan Province (104100510028).
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Hu, J., Xu, M., Hang, B. et al. Isolation and characterization of an antimicrobial peptide from bovine hemoglobin α-subunit. World J Microbiol Biotechnol 27, 767–771 (2011). https://doi.org/10.1007/s11274-010-0514-4
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DOI: https://doi.org/10.1007/s11274-010-0514-4