Abstract
Lipid accumulation by Gordonia sp. DG using sodium gluconate as carbon source in comparison with Rhodococcus opacus PD630 was studied. Maximum lipid content 80% was observed at the beginning of the stationary phase for R. opacus and 72% at the end of stationary phase for Gordonia sp. Different agro-industrial wastes were used as carbon source. The cells of the two organism accumulated lipid more than 50% of the biomass with most tested agro-industrial wastes. The maximum value was in presence of sugar cane molasses (93 and 96%) for R. opacus and Gordonia sp. respectively. Maximum triacyglycerols (TAGs), 88.9 and 57.8 mg/l, was obtained using carob and orange waste by R. opacus and Gordonia sp. respectively. The use of orange waste as carbon source by R. opacus, increased lipid unsaturation with C18:3 as the major unsaturated fatty acid. On the other hand, C22:0 and C6:0 were the dominant fatty acids (54.5% of the total identified fatty acids) produced by Gordonia sp. in presence of orange waste as carbon source. Statistical optimization of the medium revealed that maximum lipid content was achieved with 60% orange waste, 0.05 g/l ammonium chloride and 0.2 g/l magnesium sulphate.
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Acknowledgments
The authors gratefully acknowledge Prof. Dr. Steinbüchel (Muenster, Germany) for providing Rhodococcus opacus PD630 and valuable chemicals and Prof. W. Spyra, Technische Universitaet Cottbus, Germany, for providing Gordonia sp. DG., Dr. med. Habil. W. Baer and Dr. T Juretzek, Carl-Thiem-Klinikum, Cottbus, Germany, for helping in the molecular identification of Gordonia sp. DG. Thanks are also due to Prof. Dr. Ahmed S. El Sharaky, Professor of Biochemistry, Faculty of science, Alexandria University, for his valuable help in fatty acids determination and identification.
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Gouda, M.K., Omar, S.H. & Aouad, L.M. Single cell oil production by Gordonia sp. DG using agro-industrial wastes. World J Microbiol Biotechnol 24, 1703–1711 (2008). https://doi.org/10.1007/s11274-008-9664-z
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DOI: https://doi.org/10.1007/s11274-008-9664-z