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Lindane Biodegradation by Defined Consortia of Indigenous Streptomyces Strains

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Abstract

The current study aimed to compare lindane degradation by pure and mixed cultures of Streptomyces sp. Cell-free extracts were assayed for potentiating dechlorinase activity and, based on these results, consortia of two to six microorganisms were assayed for their growth on and degradation of lindane. Furthermore, the role of bacterial consortia of lindane-degrading strains was examined in lindane decontamination soil assays. Four actinobacteria, previously isolated from a pesticide-contaminated area, were selected because of their tolerance to lindane and their ability to use the pesticide as sole carbon source. These strains as well as Streptomyces sp. M7 and Streptomyces coelicolor A3 were used to study specific dechlorinase activity (SDA) and lindane removal in mixed cultures. Pure cultures presented SDA in the presence of 1.66 mg L-1 lindane as carbon source. SDA was improved by certain mixed cultures until 12 times compared with pure cultures. Mixed cultures with two, three, and four strains showed maximum lindane removal of 46% to 68%, whereas combinations of five and six strains did not efficiently remove the pesticide from the culture medium. The Streptomyces sp. A2, A5, M7, and A11 consortium presented the lowest ratio between residual lindane concentration and SDA and could be a promising tool for lindane biodegradation.

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References

  • Assaf, N. A., & Turco, R. F. (1994). Accelerated biodegradation of atrazine by a microbial consortium is possible in culture and soil. Biodegradation, 5, 29–35.

    CAS  Google Scholar 

  • Bell, D. K., Wells, H. D., & Markham, C. R. (1980). In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology, 72, 379–382.

    Article  Google Scholar 

  • Benimeli, C. S., Amoroso, M. J., Chaile, A. P., & Castro, R. G. (2003). Isolation of four aquatic streptomycetes strains capable of growth on organochlorine pesticides. Bioresource Technology, 89, 348–357.

    Article  Google Scholar 

  • Benimeli, C. S., Castro, G. R., Chaile, A. P., & Amoroso, M. J. (2006). Lindane removal induction by Streptomyces sp. M7. Journal of Basic Microbiology, 46, 348–357.

    Article  CAS  Google Scholar 

  • Benimeli, C. S., Castro, G. R., Chaile, A. P., & Amoroso, M. J. (2007). Lindane uptake and degradation by aquatic Streptomyces sp. strain M7. International Biodeterioration and Biodegradation, 59, 148–155.

    Article  CAS  Google Scholar 

  • Boltner, D., Moreno-Morillas, S., & Ramos, J. L. (2005). 16S rDNA phylogeny and distribution of lin genes in novel hexachlorocyclohexane degrading Sphingomonas strains. Environmental Microbiology, 7, 1329–1338.

    Article  CAS  Google Scholar 

  • Botella, B., Crespo, J., Rivas, A., Cerrillo, I., Olea-Serrano, M. F., & Olea, N. (2004). Exposure of women to organochlorine pesticides in Southern Spain. Environmental Research, 96, 34–40.

    Article  CAS  Google Scholar 

  • Brilhante, O. M., & Franco, R. (2006). Exposure pathways to HCH and DDT in Cidade dos Meninos and its surrounding districts of Amapa, Figueiras and Pilar, metropolitan regions of Rio de Janeiro, Brazil. International Journal of Environmental Health Research, 16, 205–217.

    Article  CAS  Google Scholar 

  • Carrillo-Pérez, E., Ruiz-Manríquez, A., & Yeomans-Reina, H. (2004). Aislamiento, identificación y evaluación de un cultivo mixto de microorganismos con capacidad para degradar DDT. Revista Internacional de Contaminación Ambiental, 20, 69–75.

    Google Scholar 

  • Cuozzo, S. A., Rollán, G. C., Abate, C. M., & Amoroso, M. J. (2009). Specific dechlorinase activity in lindane degradation by Streptomyces sp. M7. World Journal of Microbiology & Biotechnology, 25, 1539–1546.

    Article  CAS  Google Scholar 

  • De Schrijver, A., & De Mot, R. (1999). Degradation of pesticides by actinomycetes. Critical Reviews in Microbiology, 25, 85–119.

    Article  Google Scholar 

  • de Souza, M. L., Newcombe, D., Alvey, S., Crowley, D. E., Hay, A., Sadowsky, M. J., et al. (1998). Molecular basis of a bacterial consortium: Interspecies catabolism of atrazine. Applied and Environmental Microbiology, 64, 178–184.

    Google Scholar 

  • Dogra, C., Raina, V., Pal, R., Suar, M., Lal, S., Gartemann, K. H., et al. (2004). Organization of lin genes and IS6100 among different strains of hexachlorocyclohexanedegrading Sphingomonas paucimobilis: Evidence for horizontal gene transfer. Journal of Bacteriology, 186, 2225–2235.

    Article  CAS  Google Scholar 

  • Fuentes, M. S., Benimeli, C. S., Cuozzo, S. A., & Amoroso, M. J. (2010). Isolation of pesticide-degrading actinomycetes from a contaminated site: Bacterial growth, removal and dechlorination of organochlorine pesticides. International Biodeterioration and Biodegradation, 64, 434–441.

    Article  CAS  Google Scholar 

  • Guevara, C., & Zambrano, M. M. (2006). Sugarcane cellulose utilization by a defined microbial consortium. FEMS Microbiology Letters, 255, 52–58.

    Article  CAS  Google Scholar 

  • Hamer, G. (1997). Microbial consortia for multiple pollutant biodegradation. Pure and Applied Chemistry, 69, 2343–2356.

    Article  CAS  Google Scholar 

  • Herrero-Mercado, M., Waliszewski, S. M., Valencia-Quintana, R., Caba, M., Hernández-Chalate, F., García-Aguilar, E., et al. (2010). Organochlorine pesticide levels in adipose tissue of pregnant women in Veracruz, Mexico. Bulletin of Environmental Contamination and Toxicology, 84, 652–656.

    Article  CAS  Google Scholar 

  • Hirano, T., Ishida, T., Oh, K., & Sudo, R. (2007). Biodegradation of chlordane and hexachlorobenzenes in river sediment. Chemosphere, 67, 428–434.

    Article  CAS  Google Scholar 

  • Hopwood, D. A. (1967). Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriological Reviews, 31, 373–403.

    CAS  Google Scholar 

  • Hubert, C. C., Shen, Y., & Voordouw, G. (1999). Composition of toluene-degrading microbial communities from soil at different concentrations of toluene. Applied and Environmental Microbiology, 65, 3064–3070.

    CAS  Google Scholar 

  • Johri, A. K., Dua, M., Saxena, D. M., & Sethunathan, N. (2000). Enhanced degradation of hexachlorocyclohexane isomers by Sphingomonas paucimobilis. Current Microbiology, 41, 309–311.

    Article  CAS  Google Scholar 

  • Karagouni, A. D., Vionis, A. P., Baker, P. W., & Wellington, E. M. H. (1993). The effect of soil moisture content on spore germination, mycelium development and survival of a seeded streptomycete in soil. Microbial Releases, 28, 47–51.

    Google Scholar 

  • Kumar, M., & Philip, L. (2007). Biodegradation of endosulfan-contaminated soil in a pilot-scale reactor-bioaugmented with mixed bacterial culture. Journal of Environmental Science and Health. Part B, 42, 707–715.

    Article  CAS  Google Scholar 

  • Lal, R., Dadhwal, M., Kumari, K., Sharma, P., Singh, A., Kumari, H., et al. (2008). Pseudomonas sp. to Sphingobium indicum: A journey of microbial degradation and bioremediation of hexachlorocyclohexane. Indian Journal of Microbiology, 48, 3–18.

    Article  CAS  Google Scholar 

  • Lal, R., Dogra, C., Malhotra, S., Sharma, P., & Pal, R. (2006). Diversity, distribution and divergence of lin genes in hexachlorocyclohexanedegrading sphingomonads. Trends in Biotechnology, 24, 121–130.

    Article  CAS  Google Scholar 

  • Lal, R., Pandey, G., Sharma, P., Kumari, K., Malhotra, S., Pandey, R., et al. (2010). Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation. Microbiology and Molecular Biology Reviews, 74, 58–80.

    Article  CAS  Google Scholar 

  • Liu, S. U., Freyer, A. J., & Bollag, J. M. (1991). Microbial dechlorination of the herbicide metolachlor. Journal of Agricultural and Food Chemistry, 39, 631–636.

    Article  CAS  Google Scholar 

  • MacNaughton, S. J., Stephen, J. R., Venosa, A. D., Davis, G. A., Chang, Y. J., & White, D. C. (1999). Microbial population changes during bioremediation of an experimental oil spill. Applied and Environmental Microbiology, 65, 3566–3574.

    CAS  Google Scholar 

  • Mandelbaum, R. T., Wackett, L. P., & Allan, D. L. (1993). Mineralization of the s-triazine ring of atrazine by stable bacterial mixed cultures. Applied and Environmental Microbiology, 59, 1695–1701.

    CAS  Google Scholar 

  • Manickam, N., Mau, M., & Schloemann, M. (2006). Characterization of the novel HCH-degrading strain Microbacterium sp. ITRC1. Applied Microbiology and Biotechnology, 69, 580–588.

    Article  CAS  Google Scholar 

  • Mohn, W. W., Mertens, B., Neufeld, J. D., Verstraete, W., & de Lorenzo, V. (2006). Distribution and phylogeny of hexachlorocyclohexane degrading bacteria in soils from Spain. Environmental Microbiology, 8, 60–68.

    Article  CAS  Google Scholar 

  • Nagata, Y., Endo, R., Itro, M., Ohtsubo, Y., & Tsuda, M. (2007). Aerobic degradation of lindane (γ-hexachlorocyclohexane) in bacteria and its biochemical and molecular basis. Applied Microbiology and Biotechnology, 76, 741–752.

    Article  CAS  Google Scholar 

  • Nagata, Y., Futamura, A., Miyauchi, K., & Takagi, M. (1999). Two different types of dehalogenases, Lin A and Lin B, involved in γ-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26 are localized in the periplasmic space without molecular processing. Journal of Bacteriology, 181, 5409–5413.

    CAS  Google Scholar 

  • Phillips, T. M., Seech, A. G., Lee, H., & Trevors, J. T. (2001). Colorimetric assay for lindane dechlorination by bacteria. Journal of Microbiological Methods, 47, 181–188.

    Article  CAS  Google Scholar 

  • Phillips, T. M., Seech, A. G., Lee, H., & Trevors, J. T. (2005). Biodegradation of hexachlorocyclohexane (HCH) by microorganisms. Biodegradation, 16, 363–392.

    Article  CAS  Google Scholar 

  • Piñero González, M., Izquierdo Córser, P., Allara Cagnasso, M., & García Urdaneta, A. (2007). Residuos de plaguicidas organoclorados en 4 tipos de aceites vegetales. Archivos Latinoamericanos de Nutrición, 57, 397–401.

    Google Scholar 

  • Quintero, J. C., Moreira, M. T., Feijoo, G., & Lema, J. M. (2005). Anaerobic degradation of hexachlorocyclohexane isomers in liquid and soil slurry systems. Chemosphere, 61, 528–536.

    Article  CAS  Google Scholar 

  • Quintero, J. C., Moreira, M. T., Feijoo, G., & Lema, J. M. (2008). Screening of white rot fungal species for their capacity to degrade lindane and other isomers of hexachlorocyclohexane (HCH). Ciencia e Investigación Agraria, 35, 159–167.

    Article  Google Scholar 

  • Radosevich, M., Traina, S. J., Hao, Y. L., & Tuovinen, O. H. (1995). Degradation and mineralization of atrazine by a soil bacterial isolate. Applied and Environmental Microbiology, 61, 297–302.

    CAS  Google Scholar 

  • Shelton, D. R., Khader, S., Karns, J. S., & Pogell, B. M. (1996). Metabolism of twelve herbicides by Streptomyces. Biodegradation, 7, 129–136.

    Article  CAS  Google Scholar 

  • Singh, B. K., & Kuhad, R. C. (2000). Degradation of the insecticide lindane (γ-HCH) by white-rot fungi Cyathus bulleri and Phanerochaete sordida. Pest Management Science, 56, 142–146.

    Article  CAS  Google Scholar 

  • Singh, A., & Lal, R. (2009). Sphingobium ummariense sp. nov., a novel hexachlorocyclohexane degrading bacterium, isolated from HCH contaminated soil. International Journal of Systematic and Evolutionary Microbiology, 59, 162–166.

    Article  CAS  Google Scholar 

  • Siripattanakul, S., Wirojanagud, W., McEvoy, J., Limpiyakorn, T., & Khan, E. (2009). Atrazine degradation by stable mixed cultures enriched from agricultural soil and their characterization. Journal of Applied Microbiology, 106, 986–992.

    Article  CAS  Google Scholar 

  • Smith, D., Alvey, S., & Crowley, D. E. (2005). Cooperative catabolic pathways within an atrazine-degrading enrichment culture isolated from soil. FEMS Microbiology Ecology, 53, 265–273.

    Article  CAS  Google Scholar 

  • Vega, F. A., Covelo, E. F., & Andrade, M. L. (2007). Accidental organochlorine pesticide contamination of soil in Porriño, Spain. Journal of Environmental Quality, 36, 272–279.

    Article  CAS  Google Scholar 

  • Whiteley, A. S., & Bailey, M. J. (2000). Bacterial community structure and physiological state within an industrial phenol bioremediation system. Applied and Environmental Microbiology, 66, 2400–2407.

    Article  CAS  Google Scholar 

  • WHO. (1990). Public Health Impact of Pesticides Used in Agriculture. Geneva: WHO and UNEP.

    Google Scholar 

  • WHO. (2003). Health risks of persistent organic pollutants from long-range trans-boundary air pollution, Chapter 3. Hexachlorocyclohexanes (pp. 61–85). Geneva: Joint WHO/Convention Task Force on the Health Aspects of Air Pollution.

    Google Scholar 

  • Yang, C., Li, Y., Zhang, K., Wang, X., Ma, C., Tang, H., et al. (2010). Atrazine degradation by a simple consortium of Klebsiella sp. A1 and Comamonas sp. A2 in nitrogen enriched medium. Biodegradation, 21, 97–105.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge financial support of Consejo de Investigaciones de la Universidad Nacional de Tucumán (CIUNT), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), as well as technical assistance by Mr. G. Borchia.

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Authors and Affiliations

  1. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), Avenida Belgrano y Pasaje Caseros, T40001MVB, San Miguel de Tucumán, Argentina

    María Soledad Fuentes, Juliana María Sáez, Claudia Susana Benimeli & María Julia Amoroso

  2. Universidad del Norte Santo Tomás de Aquino (UNSTA), 9 de Julio 165, San Miguel de Tucumán, T4000IHC, Argentina

    Claudia Susana Benimeli & María Julia Amoroso

  3. Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Ayacucho 471, San Miguel de Tucumán, T4000INI, Argentina

    María Julia Amoroso

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  1. María Soledad Fuentes
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  2. Juliana María Sáez
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  3. Claudia Susana Benimeli
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  4. María Julia Amoroso
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Correspondence to Claudia Susana Benimeli.

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Fuentes, M.S., Sáez, J.M., Benimeli, C.S. et al. Lindane Biodegradation by Defined Consortia of Indigenous Streptomyces Strains. Water Air Soil Pollut 222, 217–231 (2011). https://doi.org/10.1007/s11270-011-0818-5

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