Skip to main content
Log in

Identification and genetic characterization of avian-origin H3N2 canine influenza viruses isolated from the Liaoning province of China in 2012

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

A total of 158 serum samples and 510 nasal swab specimens were collected between September 2010 and May 2012, from dogs exhibiting respiratory symptoms, in order to investigate the epidemiology of H3N2 canine influenza viruses (CIVs) in the Liaoning province of China. Serological surveillance demonstrated that 10.8 % (17/158) of serum samples were positive for H3N2 canine influenza. Two H3N2 influenza viruses, A/canine/Liaoning/27/2012 and A/canine/Liaoning/H6/2012, were isolated from pet dogs in 2012. Phylogenetic analysis indicated that the genes from these two viruses were closely related to those of avian-origin, H3N2 subtype CIVs from China and Thailand. Genetic analysis of eight genes revealed that these two H3N2 canine influenza isolates were highly similar (99.2–99.8 %) to the current common strains in Asia. Analysis of the genotype demonstrated that each gene of the two strains in this study had the same genotype (K, G, E, 3B, F, 2D, F, 1E) as those prevalent in H3N2 CIVs. Our findings further confirm that avian-origin H3N2 canine influenza has become established in China. Conducting extensive serological and epidemiological surveillance is necessary to develop an effective vaccine against this disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. P.C. Crawford, E.J. Dubovi, W.L. Castleman, I. Stephenson, E.P. Gibbs, L. Chen, C. Smith, R.C. Hill, P. Ferro, J. Pompey, R.A. Bright, M.J. Medina, C.M. Johnson, C.W. Olsen, N.J. Cox, A.I. Klimov, J.M. Katz, R.O. Donis, Science 310, 482–485 (2005)

    Article  PubMed  CAS  Google Scholar 

  2. D.G. Lin, S.S. Sun, L.J. Du, J.J. Ma, L.H. Fan, J. Pu, Y.P. Sun, J.Y. Zhao, H.L. Sun, J.H. Liu, J. Gen. Virol. 93, 119–123 (2012)

    Article  PubMed  CAS  Google Scholar 

  3. T. Songserm, A. Amonsin, R. Jam-on, N. Sae-Heng, N. Pariyothorn, S. Payungporn, A. Theamboonlers, S. Chutinimitkul, R. Thanawongnuwech, Y. Poovorawan, Emerg. Infect. Dis. 12, 1744–1747 (2006)

    Article  PubMed  PubMed Central  Google Scholar 

  4. Q.Q. Song, F.X. Zhang, J.J. Liu, Z.S. Ling, Y.L. Zhu, S.J. Jiang, Z.J. Xie, Vet. Microbiol. 161, 331–333 (2013)

    Article  PubMed  Google Scholar 

  5. G.J. Zhan, Z.S. Ling, Y.L. Zhu, S.J. Jiang, Z.J. Xie, Vet. Microbiol. 155, 409–416 (2012)

    Article  PubMed  CAS  Google Scholar 

  6. X.X. Sun, X.K. Xu, Q. Liu, D.J. Liang, C.Y. Li, Q.S. He, J.X. Jiang, Y.M. Cui, J. Li, L.F. Zheng, J.G. Guo, Y. Xiong, J.H. Yan, Infect. Genet. Evol. 2, 471–475 (2013)

    Article  Google Scholar 

  7. D. Song, H.J. Moon, D.J. An, H.Y. Jeoung, H. Kim, M.J. Yeom, M. Hong, J.H. Nam, S.J. Park, B.K. Park, J.S. Oh, M. Song, R.G. Webster, J.K. Kim, B.K. Kang, J. Gen. Virol. 93, 551–554 (2012)

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  8. S.J. Park, H.J. Moon, B.K. Kang, M. Hong, W. Na, J.K. Kim, H. Poo, B.K. Park, D.S. Song, J. Virol. 86, 9548–9549 (2012)

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  9. H. Wang, K. Jia, W.B. Qi, M.Z. Zhang, L. Sun, H.B. Liang, G.H. Du, L.K. Tan, Z.W. Shao, J.H. Ye, L.S. Sun, Z.P. Cao, Y. Chen, P. Zhou, S. Su, S.J. Li, Virus Genes 46, 558–562 (2013)

    Article  PubMed  CAS  Google Scholar 

  10. Y.B. Zhang, J.D. Chen, J.X. Xie, W.J. Zhu, C.Y. Wei, L.K. Tan, N. Cao, Y. Chen, M.Z. Zhang, G.H. Zhang, S.J. Li, J. Vet. Med. Sci. 75, 1061–1062 (2013)

    Article  PubMed  Google Scholar 

  11. C. Lee, D. Song, B. Kang, D. Kang, J. Yoo, K. Jung, G. Na, K. Lee, B. Park, J. Oh, Vet. Microbiol. 137, 359–362 (2009)

    Article  PubMed  Google Scholar 

  12. N. Bunpapong, N. Nonthabenjawan, S. Chaiwong, R. Tangwangvivat, S. Boonyapisitsopa, W. Jairak, R. Tuanudom, D. Prakairungnamthip, S. Suradhat, R. Thanawongnuwech, A. Amonsin, Virus Genes 48, 56–63 (2014)

    Article  PubMed  CAS  Google Scholar 

  13. Y. Lin, Y. Zhao, X. Zeng, C. Lu, Y. Liu, Vet. Microbiol. 158, 247–258 (2012)

    Article  PubMed  CAS  Google Scholar 

  14. Y. Sun, S. Sun, J. Ma, Y. Tan, L. Du, Y. Shen, Q. Mu, J. Pu, D. Lin, J. Liu, Virology 435, 301–307 (2013)

    Article  PubMed  CAS  Google Scholar 

  15. S. Su, Z.G. Yuan, J.D. Chen, J.X. Xie, H.T. Li, Z. Huang, M.Z. Zhang, G.H. Du, Z.M. Chen, L.Q. Tu, Y.F. Zou, J.H. Miao, H. Wang, K. Jia, S.J. Li, Virus Genes 46, 554–557 (2013)

    Article  PubMed  CAS  Google Scholar 

  16. K. Tamura, J. Dudley, M. Nei, S. Kumar, Mol. Biol. Evol. 24, 1596–1599 (2007)

    Article  PubMed  CAS  Google Scholar 

  17. G. Lu, T. Rowley, R. Garten, R.O. Donis, Nucleic Acids Res. 35, 275–279 (2007)

    Article  Google Scholar 

  18. M. Matrosovich, N. Zhou, Y. Kawaoka, R. Webster, J. Virol. 73, 1146–1155 (1999)

    PubMed  CAS  PubMed Central  Google Scholar 

  19. M. Muranaka, T. Yamanaka, Y. Katayama, K. Hidari, H. Kanazawa, T. Suzuki, K. Oku, T. Oyamada, J. Vet. Med. Sci. 73, 125–127 (2011)

    Article  PubMed  Google Scholar 

  20. N.K. Sauter, J.E. Hanson, G.D. Glick, J.H. Brown, R.L. Crowther, S.J. Park, J.J. Skehel, D.C. Wiley, Biochemistry 31, 9609–9621 (1992)

    Article  PubMed  CAS  Google Scholar 

  21. C.F. Basler, A.H. Reid, J.K. Dybing, T.A. Janczewski, T.G. Fanning, H. Zheng, M. Salvatore, M.L. Perdue, D.E. Swayne, A. Garcia-Sastre, P. Palese, J.K. Taubenberger, Proc. Natl. Acad. Sci. USA 98, 2746–2751 (2001)

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  22. J.C. Obenauer, J. Denson, P.K. Mehta, X. Su, S. Mukatira, D.B. Finkelstein, X. Xu, J. Wang, J. Ma, Y. Fan, K.M. Rakestraw, R.G. Webster, E. Hoffmann, S. Krauss, J. Zheng, Z. Zhang, C.W. Naeve, Science 311, 1576–1580 (2006)

    Article  PubMed  CAS  Google Scholar 

  23. S.H. Seo, E. Hoffmann, R.G. Webster, Virus Res. 103, 107–113 (2004)

    Article  PubMed  CAS  Google Scholar 

  24. D. Jackson, M.J. Hossain, D. Hickman, D.R. Perez, R.A. Lamb, Proc. Natl. Acad. Sci. USA 105, 4381–4386 (2008)

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  25. R. Gao, B. Cao, Y. Hu, Z. Feng, D. Wang, W. Hu, J. Chen, Z. Jie, H. Qiu, K. Xu, X. Xu, H. Lu, W. Zhu, Z. Gao, N. Xiang, Y. Shen, Z. He, Y. Gu, Z. Zhang, Y. Yang, X. Zhao, L. Zhou, X. Li, S. Zou, Y. Zhang, L. Yang, J. Guo, J. Dong, Q. Li, L. Dong, Y. Zhu, T. Bai, S. Wang, P. Hao, W. Yang, J. Han, H. Yu, D. Li, G.F. Gao, G. Wu, Y. Wang, Z. Yuan, Y. Shu, N. Engl. J. Med. 368, 1888–1897 (2013)

    Article  PubMed  CAS  Google Scholar 

  26. T.M. Uyeki, N.J. Cox, N. Engl. J. Med. 368, 1862–1864 (2013)

    Article  PubMed  CAS  Google Scholar 

  27. H. Kim, D. Song, H. Moon, M. Yeom, S. Park, M. Hong, W. Na, R.J. Webby, R.G. Webster, B. Park, J.K. Kim, B. Kang, Influenza Other Respi. Viruses 7, 265–270 (2012)

    Article  Google Scholar 

  28. N. Lei, Z.G. Yuan, S.F. Huang, D.W. Zhang, A.G. Zhang, B.H. Huang, G.H. Zhang, S.J. Li, Vet. Microbiol. 160, 481–483 (2012)

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This study was partly supported by the Heilongjiang Postdoctoral Financial Assistance (LBH-Z13043), the Chinese Special Fund for Agro-scientific Research in the Public Interest (201303046), the Open Fund of State Key Laboratory of Veterinary Biotechnology of Harbin veterinary research institute (SKLVBF201404), and the Basic Scientific Research Operation Cost of State-leveled Public Welfare Scientific Research Courtyard (0302014014).

Conflict of interests

The authors have declared that they have no competing interests.

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Donghua Xia or Ming Liu.

Additional information

Xinyan Yang, Chunguo Liu and Fei Liu contributed equally to this work.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, X., Liu, C., Liu, F. et al. Identification and genetic characterization of avian-origin H3N2 canine influenza viruses isolated from the Liaoning province of China in 2012. Virus Genes 49, 342–347 (2014). https://doi.org/10.1007/s11262-014-1092-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-014-1092-6

Keywords

Navigation