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Sequence and phylogenetic analysis of the S1 genome segment of turkey-origin reoviruses

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Abstract

Based on previous reports characterizing the turkey-origin avian reovirus (TRV) σB (σ2) major outer capsid protein gene, the TRVs may represent a new group within the fusogenic orthoreoviruses. However, no sequence data from other TRV genes or genome segments has been reported. The σC protein encoded by the avian reovirus S1 genome segment is the cell attachment protein and a major antigenic determinant for avian reovirus. The chicken reovirus S1 genome segment is well characterized and is well conserved in viruses from that species. This report details the amplification, cloning and sequencing of the entire S1 genome segment from two and the entire coding sequences of the σC, p10 and p17 genes from an additional five TRVs. Sequence analysis reveals that of the three proteins encoded by the TRV S1 genome segment, σC shares at most 57% amino acid identity with σC from the chicken reovirus reference strain S1133, while the most similar p10 and p17 proteins share 72% and 61% identity, respectively, with the corresponding S1133 proteins. The most closely related mammalian reovirus, the fusogenic Nelson Bay reovirus, encodes a σC protein that shares from 25% to 28% amino acid identity with the TRV σC proteins. This report supports the earlier suggestion that the TRVs are a separate virus species within the Orthoreovirus genus, and may provide some insight into TRV host specificity and pathogenesis.

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Authors and Affiliations

  1. Southeast Poultry Research Laboratory, USDA-ARS, 934 College Station Road, Athens, GA, 30605, USA

    J. Michael Day, Mary J. Pantin-Jackwood & Erica Spackman

Authors
  1. J. Michael Day
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  2. Mary J. Pantin-Jackwood
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  3. Erica Spackman
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Corresponding author

Correspondence to Erica Spackman.

Additional information

NC/SEP–R44/03 S1 genome segment DQ525419

NC/98 S1 genome segment DQ995806

TRV sigmaC sequences DQ996601–DQ996605

TRV p10 sequences DQ996606–DQ996610

TRV p17 sequences DQ996611–DQ996615

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Day, J.M., Pantin-Jackwood, M.J. & Spackman, E. Sequence and phylogenetic analysis of the S1 genome segment of turkey-origin reoviruses. Virus Genes 35, 235–242 (2007). https://doi.org/10.1007/s11262-006-0044-1

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  • DOI: https://doi.org/10.1007/s11262-006-0044-1

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