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Biophysical and biochemical investigation on the binding of a manganese–cyanonitrosyl complex with DNA

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Abstract

The interaction of a cyanonitrosyl–manganate complex with DNA has been studied by u.v.–vis spectroscopy, circular dichroism, fluorescence and gel electrophoresis techniques. As the DNA 260 nm band remained insensitive to interaction, the interaction ratio was determined by monitoring the CN→ Mn LMCT band observed at 222 nm, and the interaction ratio was found to be in the complex (D): DNA (P)=1:0.30 from the spectrophotometric titration. The above-mentioned physical measurements indicate that the binding mode is not intercalative and the cyanonitrosyl system is a groove binder.

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References

  1. S. Moncada R.M.J. Palmer EA. Higgis (1991) Pharmacol. Rev. 43 109

    Google Scholar 

  2. F. Murrad (1999) Angew. Chem., Int. Ed. Engl. 38 1856

    Google Scholar 

  3. LJ. Ignaro (1999) Angew. Chem., Int. Ed. Engl. 38 1882

    Google Scholar 

  4. RF. Furchgott (1999) Angew. Chem., Int. Ed. Engl. 38 1870

    Google Scholar 

  5. S. Pfeiffer B. Mayer B. Hemmens (1999) Angew. Chem., Int. Ed. Engl. 38 1714

    Google Scholar 

  6. KM. Miranda RW. Nims DD. Thomas MG. Espey D. Citrin MD. Bartberger N. Paolocci JM. Fukuto M. Feelisch DA. Wink (2003) J. Inorg. Biochem. 93 52

    Google Scholar 

  7. LN. Ji X.H. Zou JG. Liu (2001) J. Inorg. Chem. Rev. 216 513

    Google Scholar 

  8. BA. Jackson V.Y. Alekseyev JK. Barton (1999) Biochemistry. 38 4655

    Google Scholar 

  9. PJ. Dandlier R.E. Holmlin JK. Barton (1997) Science. 274 1465

    Google Scholar 

  10. DB. Hall R.E. Holmlin JK. Barton (1996) Nature. 382 731

    Google Scholar 

  11. L. Mishra AK. Yadaw S. Srivastava AB. Patel (2000) New J. Chem. 24 505

    Google Scholar 

  12. A.M. Pyle JK. Barton (1990) Prog. Inorg. Chem. 38 413

    Google Scholar 

  13. JK. Barton (1986) Science. 233 727

    Google Scholar 

  14. RT. Watson N. Desai J. Wildsmith JF. Wheeler (1999) Inorg. Chem. 38 2683

    Google Scholar 

  15. A. Ambroise BG. Maiya (2000) Inorg. Chem. 39 4256

    Google Scholar 

  16. DD. Perrin W.L.F. Armarego DR. Perrin (1966) Purification of Laboratory Chemicals Pergamon Press New York

    Google Scholar 

  17. J. Marmur (1961) J. Mol. Biol. 3 208

    Google Scholar 

  18. MF. Reichmann SA. Rice C.A. Thomas P. Doty (1954) J. Am. Chem. Soc. 76 3047

    Google Scholar 

  19. R. Bhattacharyya, N. Ghosh and G.P. Bhattacharjee, J. Chem. Soc. Dalton Trans., 1963 (1989)

  20. C.J. Burrows JG. Muller (1998) Chem. Rev. 98 1109

    Google Scholar 

  21. SA. Tysoe RJ. Morgan A.D. Bakerand TC. Streakas (1993) J. Phys. Chem. 97 1707

    Google Scholar 

  22. TM. Kelly AB. Tossi D.J. McConnel TC. Streakas (1985) Nucleic Acids Res. 13 6017

    Google Scholar 

  23. RF. Pasternack E.J. Gibbs JJ. Villafranca (1983) Biochemistry. 22 2406

    Google Scholar 

  24. A. Wolfe G.H. Shimer T. Meehan (1987) Biochemistry. 26 6392

    Google Scholar 

  25. J.B. LePecq C. Paoletti (1967) J. Mol. Biol. 27 87

    Google Scholar 

  26. B.C. Baugley M. LeBret (1984) Biochemistry. 23 937

    Google Scholar 

  27. DS. Sigman DR. Graham L.E. Marshall KA. Reich (1980) J. Am. Chem. Soc. 102 5419

    Google Scholar 

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Correspondence to Kalyan K. Mukherjea or Ramgopal Bhattacharyya.

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Chowdhury, S.R., Mukherjea, K.K. & Bhattacharyya, R. Biophysical and biochemical investigation on the binding of a manganese–cyanonitrosyl complex with DNA. Transition Met Chem 30, 601–604 (2005). https://doi.org/10.1007/s11243-005-4052-0

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  • DOI: https://doi.org/10.1007/s11243-005-4052-0

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