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Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis

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Abstract

Endogenous bacterial contaminants isolated from infected cultures of Ilex dumosa nodal segments were identified as Stenotrophomonas maltophilia and Achromobacter sp. using 16S rDNA analysis. A range of antibiotics with different mechanism of actions and the commercial biocide PPM™ were tested for their capacity to repress the growth of Gram negative bacteria grown in liquid medium during the establishment phase of temporal immersion systems. The best results were obtained with the addition of 0.5 mg ml−1 cefotaxime to the culture media obtaining 100% of uncontaminated cultures without suppress of shoot growth.

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Abbreviations

Ap:

Ampicilline

Cf:

Cefotaxime

Cm:

Chloramphenicol

Gm:

Gentamycin

Km:

Kanamycin

Sm:

Streptomycin

PPM™:

Plant preservative mixture™

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Acknowledgements

The authors are gratefully indebted to the supporting funding from ANPCyT (Grant no. 08-10849), CONICET, and Establecimiento Las Marías S.A. We extend our deep appreciation to Prof. Elsie O’Connor for English language checking. We thank anonymous reviewers for their critical comments.

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Authors and Affiliations

  1. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Sgto. Cabral 2131, CC: 209, W3402BKG, Corrientes, Argentina

    Claudia Luna, Mónica Collavino, Luis Mroginski & Pedro Sansberro

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  1. Claudia Luna
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  2. Mónica Collavino
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  3. Luis Mroginski
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  4. Pedro Sansberro
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Correspondence to Pedro Sansberro.

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Luna, C., Collavino, M., Mroginski, L. et al. Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis. Plant Cell Tiss Organ Cult 95, 13–19 (2008). https://doi.org/10.1007/s11240-008-9408-7

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  • DOI: https://doi.org/10.1007/s11240-008-9408-7

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