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Kinetic Analysis of the Slow Inactivation of Rubisco During Catalysis: Effects of Temperature, O2 and Mg++

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Abstract

The effect of temperature, O2 and Mg++ on the kinetic characteristics of the slow inactivation (fallover) of Rubisco isolated from spinach (Spinacia oleracea L.) was determined. Comparing 25 and 45 °C, the rate of activity decline of Rubisco increased by 20-fold, but the final ratio of steady state to initial activity increased from 0.38 to 0.62, respectively. Low CO2 increased the extent of fallover but only caused a marginal increase in fallover rate in agreement with results reported previously. In contrast, increased O2 during catalysis significantly increased only the fallover rate. Low Mg++ greatly increased the fallover of Rubisco both in rate and extent. Rubisco carbamylation was assayed using a new separation technique and it revealed that a loss of carbamylation largely accounted for the increased fallover observed with low Mg++. In conclusion, Rubisco fallover is facilitated by high temperature, low concentration of CO2 or Mg++, and high O2. The physiological importance of these factors in affecting Rubisco fallover and contributing to photosynthetic inhibition at high temperatures in planta are discussed.

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Abbreviations

Fallover:

slow inactivation of Rubisco during catalysis

Rubisco:

ribulose-1,5-bisphosph ate carboxylase/oxygenase

ribulose-P2 :

ribulose-1,5-bisphosphate

pentodiulose-P2 :

glycero-2,3-diulose-1,5-bisphosphate

xylulose-P2 :

xylulose-1,5-bisphosphate

carboxytetritol-P2 :

2′-carboxytetritol-1,4-bisph osphate

carboxyarabinitol-P2 :

2-carboxyarabinitol-1,5-bisphosphate

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Authors and Affiliations

  1. U.S. Department of Agriculture, Photosynthesis Research Unit, Agricultural Research Service, 61801 , IL, Urbana, USA

    Kangmin Kim & Archie R. Portis Jr.

  2. Department of Crop Sciences, University of Illinois, 190 E. R. Madigan Laboratory, 1201 W Gregory Dr., 61801, IL, Urbana, USA

    Archie R. Portis Jr.

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  1. Kangmin Kim
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  2. Archie R. Portis Jr.
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Correspondence to Archie R. Portis Jr..

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Kim, ., Portis, A.R. Kinetic Analysis of the Slow Inactivation of Rubisco During Catalysis: Effects of Temperature, O2 and Mg++ . Photosynth Res 87, 195–204 (2006). https://doi.org/10.1007/s11120-005-8386-4

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