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DORNRÖSCHEN-LIKE, an AP2 gene, is necessary for stamen emergence in Arabidopsis

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Abstract

To identify novel genes in petal and stamen development, a genetic screen was carried out for enhancers of the unusual B class mutant pistillata-5 (pi-5). In pi-5 flowers, second whorl organs develop as sepals rather than petals, but third whorl stamens are normal. One pi-5 enhancer, dornröschen-like-2 (drnl-2), results in third whorl positions developing as filamentous organs. In addition to enhancing the pi-5 phenotype, drnl-2 mutants also exhibit a phenotype in a wild-type PI background. Although stamen primordia are morphologically visible during early stages of flower development, they fail to enlarge in drnl-2 mutants. DRNL, which encodes a single AP2 domain protein, is expressed in a dynamic pattern in the embryo, seedling, and flower. Analysis of both the drnl-2 mutant phenotype and the DRNL expression pattern in flowers suggests that DRNL plays a critical role in stamen emergence in Arabidopsis.

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Abbreviations

IAA:

Indole-3-acetic acid

EMS:

Ethylmethanesulfonate

kb:

Kilobase

ARE:

Auxin response elements

ARF:

Auxin response factor

SAM:

Shoot apical meristem

X-gluc:

5-bromo-4chloro-3indolyl-beta-O-glucuronic acid

SEM:

Scanning electron microscope

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Acknowledgements

We thank John Chandler and Wolfgang Werr for sharing the drnl-1 allele prior to publication, and for productive discussions. We also thank Jason Reed for the arf mutant lines, Hong Ma for his opinion of the anther expression pattern in DRNL, Chuck Daghlian for his help with SEM, Eileen Piwarzyk and Stacey King for comments on the manuscript, and the Arabidopsis Biological Resource Center for seed stocks. This work was supported by a grant from the US National Science Foundation (IBN-0516736).

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  1. Yingzhen Yang

    Present address: Section of Cell and Developmental Biology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0116, USA

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  1. Department of Biological Sciences, Dartmouth College, Hanover, NH, 03755, USA

    Anwesha Nag, Yingzhen Yang & Thomas Jack

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  1. Anwesha Nag
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Correspondence to Thomas Jack.

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Nag, A., Yang, Y. & Jack, T. DORNRÖSCHEN-LIKE, an AP2 gene, is necessary for stamen emergence in Arabidopsis. Plant Mol Biol 65, 219–232 (2007). https://doi.org/10.1007/s11103-007-9210-7

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