Abstract
The changes in growth and photosynthetic performance of two wheat (Triticum aestivum L.) cultivars (Bolal-2973 and Atay-85) differing in their sensitivity to boron (B) toxicity were investigated under toxic B conditions. Eight-day old seedlings were exposed to highly toxic B concentrations (5, 7.5, and 10 mM H3BO3) for 5 and 9 days. Fast chlorophyll a fluorescence kinetics was determined and analysed using JIP test. Growth parameters, tissue B contents, and membrane damage were measured at two stress durations. The photochemical performance of PSII was hindered more in the sensitive cultivar (Atay-85) than that of the tolerant one (Bolal-2973) under B toxicity. The increase in the B concentration and stress duration caused membrane leakage in both cultivars. However, higher membrane damage was observed in Atay-85 compared to Bolal-2973. Additionally, significant reduction of growth parameters was observed in both cultivars at toxic B concentrations. The accumulation of B was higher in shoots than in roots of both cultivars. Nevertheless, Atay-85 translocated more B from roots to leaves compared to Bolal-2973. The advantages of certain JIP test parameters were demonstrated for evaluation of PSII activity in plants exposed to B stress. Evaluation of photosynthetic performance by JIP test as well as assessment of growth and tissue B content might be used to determine the effects of B toxicity in wheat. The results indicated lesser sensitivity to B toxicity in Bolal-2973 compared to Atay-85.
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Abbreviations
- ABS:
-
absorption
- CS:
-
cross section
- ET:
-
electron transfer/transport
- F0 :
-
initial fluorescence intensity
- FM :
-
maximal fluorescence intensity
- ICP-ASE:
-
inductively coupled plasma-atomic emission spectroscopy
- OJIP:
-
fluorescence transient with O-J-I-P phases
- PI:
-
performance index
- QA :
-
primary quinone acceptor of photosystem II
- QB :
-
secondary quinone acceptor of photosystem II
- RC:
-
reaction centre
- TR:
-
trapping flux
- φE0 or ET0/ABS:
-
quantum yield of electron transport
- φP0 or TR0/ABS:
-
maximum quantum yield of primary photochemistry
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Acknowledgements: This work was supported by the National Boron Research Institute, Turkey (Project BOREN-2009-Ç0217).
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Öz, M.T., Turan, Ö., Kayihan, C. et al. Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test. Photosynthetica 52, 555–563 (2014). https://doi.org/10.1007/s11099-014-0065-2
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DOI: https://doi.org/10.1007/s11099-014-0065-2