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Beneficial Effects of Minocycline on Cuprizone Induced Cortical Demyelination

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Abstract

In this study, we investigated the potential of minocycline to influence cuprizone induced demyelination in the grey and white matter. To induce demyelination C57BL/6 mice were fed with cuprizone for up to 6 weeks and were analysed at different timepoints (week 0, 4, 5, 6). Mice treated with minocycline had less demyelination of the cortex and corpus callosum compared with sham treated animals. In the cortex decreased numbers of activated and proliferating microglia were found after 6 weeks of cuprizone feeding, while there were no significant effects for microglial infiltration of the corpus callosum. In addition to the beneficial effects on demyelination, minocycline prevented from motor coordination disturbance as shown in the beam walking test. For astrogliosis and the numbers of OPC and oligodendrocytes no treatment effects were found. In summary, minocycline treatment diminished the course of demyelination in the grey and white matter and prevented disturbances in motor coordination.

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Acknowledgments

This work has been supported by the Georg-Christoph-Lichtenberg Fellowship by the State of Lower Saxony. We thank I. Cierpka-Leja for excellent technical assistance.

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Authors and Affiliations

  1. Department of Neurology, Hannover Medical School, Carl-Neuberg-Str-1, 30625, Hanover, Germany

    Thomas Skripuletz, Elvira Miller, Darius Moharregh-Khiabani, Alexander Blank, Refik Pul, Viktoria Gudi, Corinna Trebst & Martin Stangel

  2. Center for Systems Neuroscience, Hannover, Germany

    Viktoria Gudi & Martin Stangel

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  1. Thomas Skripuletz
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  2. Elvira Miller
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  3. Darius Moharregh-Khiabani
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Correspondence to Martin Stangel.

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Skripuletz, T., Miller, E., Moharregh-Khiabani, D. et al. Beneficial Effects of Minocycline on Cuprizone Induced Cortical Demyelination. Neurochem Res 35, 1422–1433 (2010). https://doi.org/10.1007/s11064-010-0202-7

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  • DOI: https://doi.org/10.1007/s11064-010-0202-7

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