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Inhibition of human Neuroblastoma in SCID mice by low-dose of selective Cox-2 inhibitor Nimesulide

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Summary

Neuroblastoma is the most common solid tumor of infants and carries a poor prognosis especially in advanced stages. The present recommended therapies carry a high risk of side effects that is associated with long-term morbidity. We evaluated the efficacy of a low dose of the selective cyclooxygenase-2 inhibitor Nimesulide in preventing human Neuroblastoma tumor growth in Severe Combined Immune-deficient mice. Mice containing established tumors (SH-SY5Y cells) treated with 20 mg/kg Nimesulide every 4th day beginning on day 1 of cell injections resulted in a 65% reduction of tumor growth compared to the DMSO treated control mice (P<0.05) but did not significantly reduce tumor growth when Nimesulide was started once tumors reached 1cm. There was a reduction in the level of cyclooxygenase-2 protein and induction of effecter caspases in tumors treated with Nimesulide. However, there was no change in the levels of X-Inhibitor-of-Apoptosis-Protein, Smac/Diablo, or proteins of the PI3/Akt pathway following Nimesulide treatment. In Conclusion, low doses of Nimesulide can potentially be used as a chemopreventive agent for human Neuroblastoma.

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Author notes

  1. Bhupesh Parashar

    Present address: Department of Radiation Oncology, Weill Cornell Medical Center,, New York Presbyterian Hospital, 525, E 68th Street, New York, NY, 10021, USA

Authors and Affiliations

  1. Department of Radiation Oncology, Montefiore Medical Center, Bronx, NY, USA

    Bhupesh Parashar

  2. Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA

    Bridget Shafit-Zagardo

  3. Department of Radiation Oncology, Weill Cornell Medical Center,, New York Presbyterian Hospital, 525, E 68th Street, New York, NY, 10021, USA

    Bridget Shafit-Zagardo

Authors
  1. Bhupesh Parashar
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  2. Bridget Shafit-Zagardo
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Correspondence to Bridget Shafit-Zagardo.

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Parashar, B., Shafit-Zagardo, B. Inhibition of human Neuroblastoma in SCID mice by low-dose of selective Cox-2 inhibitor Nimesulide. J Neurooncol 78, 129–134 (2006). https://doi.org/10.1007/s11060-005-9079-8

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  • DOI: https://doi.org/10.1007/s11060-005-9079-8

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