Summary
Autologous tumor-specific T lymphocyte (ATTL) lines were derived from the peripheral blood mononuclear cells (PBMC) of a healthy volunteer with human leukocyte antigen (HLA) -A*0201. These lines were achieved using interleukins -1β, -2, -4, and -6 and the p53-based peptide from the 264–272 sequence of the wild-type p53 protein with a strong affinity against HLA-A*0201.
;The frequencies of CD3+, CD4+, and CD8+ lymphocytes were 94–96%, 30–34%, and 69–74%, respectively. ATTLs killed most of the T2 cells pulsed with p53-derived peptide, but not against the T2 cells non-pulsed or pulsed with an irrelevant peptide. ATTLs also killed TKB-14 cells, which have been derived from human glioblastoma multiforme, and exhibited HLA-A*0201 molecule and immunohistochemical accumulation of p53 protein. These cytotoxic activities were inhibited by anti-CD3, anti-CD8, and anti-class I antibodies.
These findings suggested that these ATTL lines might include CTL populations, which could recognize p53-derived peptide on HLA-A*0201 and the p53-based peptide may play as an antigen on HLA-A*0201. When tumor antigens would be more analyzed in the future, ATTL could be induced without the primary-cultured cells from tumor tissue and could be applied for cancer therapy.
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References
FP Holladay T Heitz GW Wood (1992) ArticleTitleAntitumor activity against established intracerebral glioma exhibited by cytotoxic T lymphocytes, but not lymphokine-activated killer cells J Neurosurg 77 757–762 Occurrence Handle1:STN:280:ByyD3Mbls1w%3D Occurrence Handle1403119
H Ikarashi K Fujita K Takakuwa S Kodama A Tokunaga T Takahashi K Tanaka (1994) ArticleTitleImmunomodulation in patients with epithelial ovarian cancer after adoptive transfer of tumor-infiltrating lymphocytes Cancer Res 54 190–196 Occurrence Handle1:STN:280:ByuD1cvmvFY%3D Occurrence Handle8261438
Y Kawakami S Eliyahu CH Delgado PF Robbins K Sakaguchi E Appella JR Yannelli GJ Adema T Miki SA Rosenberg (1994) ArticleTitleIdentification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection Proc Natl Acad Sci USA 91 6458–6462 Occurrence Handle1:CAS:528:DyaK2cXmslygtb0%3D Occurrence Handle8022805
SA Rosenberg MT Lotze LM Muul AE Chang FP Avis S Leitman WM Linehan CN Robertson RE Lee JT Rubin CA Seipp CG Simpson DE White (1987) ArticleTitleA progress report on the treatment of 157 patients with advanced cancer using lymphokine-activated killer cells and interleukin-2 or high-dose interleukin-2 alone New Engl J Med 316 889–897 Occurrence Handle1:STN:280:BiiC2cvltFI%3D Occurrence Handle3493432
SA Rosenberg P Aebersold K Cornetta A Kasid RA Morgan R Moen EM Karson MT Lotze JC Yang SL Topalian MJ Merino K Culver AD Miller RM Blaese WF Anderson (1990) ArticleTitleGene transfer into humans-immunotherapy of patients with advanced melanoma, using tumor infiltrating lymphocytes modified by retroviral gene transduction New Engl J Med 323 570–578 Occurrence Handle1:CAS:528:DyaK3MXhsl2ls7c%3D Occurrence Handle2381442
CL Slingluff AL Cox JM Stover MM Moore DF Hunt VM Engelhard (1994) ArticleTitleCytotoxic T-lymphocyte response to autologous human squamous cell cancer of the lung: epitope reconstitution with peptides extracted from HLA-Aw68 Cancer Res 54 2731–2737 Occurrence Handle1:CAS:528:DyaK2cXjt1Sqtbc%3D Occurrence Handle7513255
BA Tjoa DM Kranz (1994) ArticleTitleGeneration of cytotoxic T-lymphocytes to a self-peptide/class I complex: a model for peptide-mediated tumor rejection Cancer Res 54 204–208 Occurrence Handle1:CAS:528:DyaK2cXnsFShug%3D%3D Occurrence Handle7505197
H Tsurushima SQ Liu K Tuboi A Matsumura Y Yoshii T Nose K Saijo T Ohno (1999) ArticleTitleReduction of end-stage malignant gliomas by injection with autologous cytotoxic T lymphocytes Jpn J Cancer Res 90 536–545 Occurrence Handle1:CAS:528:DyaK1MXjsVOmu7s%3D Occurrence Handle10391094
K Tsuboi K Saijo E Ishikawa H Tsurushima S Takano Y Morishita T Ohno (2003) ArticleTitleEfffects of local injection of ex vivo expanded autologous tumor-specific T lymphocytes in cases with recurrent malignant gliomas Clin Cancer Res 15 3294–3302
NJ Crowley TL Darrow MA Quinnallen HF Seigler (1991) ArticleTitleMHC-restricted recognition of autologous melanoma by tumor-specific cytotoxic T cells – evidence for restriction by a diminant HLA-A allele J Immunol 146 1692–1699 Occurrence Handle1:STN:280:By6C3s3gsVc%3D Occurrence Handle1671580
T Wolfel W Herr P Coulie U Schmitt KHM Zumbuschenfelde A Knuth (1993) ArticleTitleLysis of human pancreatic adenocarcinoma cells by autologous HLA-class I-restricted cytotoxic T-lymphocyte (CTL) clones Int J Cancer 54 636–644 Occurrence Handle1:STN:280:ByyB1MzjvVc%3D Occurrence Handle8514455
H Tsurushima SQ Liu K Tsuboi Y Yoshii T Nose T Ohno (1996) ArticleTitleInduction of human cytotoxic T lymphocytes against minced tissues of glioblastoma multiforme J Neurosurg 84 258–263 Occurrence Handle1:STN:280:BymC2M3jvFU%3D Occurrence Handle8592229
K Horiuchi H Tsurushima BS Kim SQ Liu K Saijo Y Saijo T Nukiwa N Nomura M Matsumura T Ohno (1998) ArticleTitleExpansion of human autologous cytotoxic T lymphocytes on fixed target tumor cells Cytotechnology 26 119–124 Occurrence Handle10.1023/A:1007903614475
SQ Liu K Saijo T Todoroki T Ohno (1995) ArticleTitleInduction of human autologous cytotoxic T lymphocytes on formalin-fixed and paraffin-embedded tumor sections Nat Med 1 267–271 Occurrence Handle1:CAS:528:DyaK2MXksVGns78%3D Occurrence Handle7585045
SQ Liu H Shiraiwa K Kawai H Hayashi H Akaza BS Kim A Oki M Nishida T Kubo K Hashizaki K Saijo T Ohno (1996) ArticleTitleTumor-specific autologous cytotoxic T lymphocytes from tissue sections Nat Med 2 1283 Occurrence Handle1:CAS:528:DyaK28XntlymsrY%3D Occurrence Handle8946818
S Gnjatic BB Depaillerets JG Guillet J Choppin (1995) ArticleTitleMapping and ranking of potential cytotoxic T epitopes in the p53 protein: effect of mutations and polymorphism on peptide binding to purified and refolded HLA molecules Eur J Immunol 25 1638–1642 Occurrence Handle1:CAS:528:DyaK2MXms1Klsrg%3D Occurrence Handle7542198
JGA Houbiers HW Nijman SH Vanderburg JW Drijfhout P Kenemans CJH Vandevelde A Brand F Momburg WM Kast CJM Melief (1993) ArticleTitleIn vitro induction of human cytotoxic T lymphocytes responses against peptide of mutant and wild-type p53 Eur J Immunol 23 2072–2077 Occurrence Handle1:CAS:528:DyaK2cXivVyguw%3D%3D Occurrence Handle8370389
G Stuber GH Leder WJ Storkus MT Lotze S Modrow L Szekely H Wolf E Klein K Karre G Klein (1994) ArticleTitleIdentification of wild-type and mutant p53 peptide binding to HLA-A2 assessed by a novel major histocompatibility complex class I peptide binding assay Eur J Immunol 24 765–768 Occurrence Handle1:CAS:528:DyaK2cXis1Olt7w%3D Occurrence Handle8125143
M Ropke J Hald P Guldberg J Zeuthen L Norgaard L Fugger A Svejgaard S Vanderburg HW Nijman CJ Melief MH Claesson (1996) ArticleTitleSpontaneous human squamous cell carcinoma are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide Proc Natl Acad Sci USA 93 1404–1407 Occurrence Handle10.1073/pnas.93.25.14704
S Gnjatic Z Cai M Viguier S Chouaib JG Guillet J Choppin (1998) ArticleTitleAccumulation of the p53 protein allows recognition by human CTL of a wild-type p53 epitope presented by breast carcinomas and melanomas J Immunol 160 328–333 Occurrence Handle1:CAS:528:DyaK1cXhtFWguw%3D%3D Occurrence Handle9551988
LV Crawford DC Pim RD Bulbrook (1982) ArticleTitleDetection of antibodies against the cellular protein p53 in sera from patients with breast cancer Int J Cancer 30 403–408 Occurrence Handle1:STN:280:BiyD2MrptFQ%3D Occurrence Handle6292117
T Soussi (1996) ArticleTitleThe humoral response to the tumor-suppressor gene-product p53 in human cancer: implications for diagnosis and therapy Immunol Today 17 354–356 Occurrence Handle10.1016/0167-5699(96)30019-4 Occurrence Handle1:CAS:528:DyaK28XkvFGktLc%3D Occurrence Handle8783493
R Soong PD Robbins BR Dix F Grieu B Lim S Knowles KE Williams GR Turbett AK House BJ Iacopetta (1996) ArticleTitleConcordance between p53 protein overexpression and gene mutation in a large series of common human carcinomas Hum Pathol 27 1050–1055 Occurrence Handle10.1016/S0046-8177(96)90282-8 Occurrence Handle1:STN:280:ByiD2cjnsVw%3D Occurrence Handle8892589
K Watanabe K Sato W Biernat O Tachibana K vonAmmon N Ogata Y Yonekawa P Kleihues H Ohgaki (1997) ArticleTitleIncidence and timing of p53 mutations during astrocytoma progression in patients with multiple biopsies Clin Cancer Res 3 523–530 Occurrence Handle1:CAS:528:DyaK2sXislGitLY%3D Occurrence Handle9815715
M Ropke M Regner MH Claesson (1995) ArticleTitleT cell-mediated cytotoxity against p53-protein derived peptides in bulk and limiting dilution cultures of healthy donors Scand J Immunol 42 98–103 Occurrence Handle1:CAS:528:DyaK2MXotFOjsLo%3D Occurrence Handle7543215
HW Nijman JGA Houbiers SH Vanderburg MPM Vierboom P Kenemans WM Kast CJM Melief (1993) ArticleTitleCharacterization of cytotoxic T lymphocyte epitopes of a self-protein, p53, and a non-self-protein, influenza matrix: relationship between major histocompatibility complex peptide binding affinity and immune responsiveness to peptides J Immunother 14 121–126 Occurrence Handle1:CAS:528:DyaK2cXhvV2is7g%3D Occurrence Handle7506574
K Chikamatsu K Nakano WJ Storkus E Appella MT Lotze TL Whiteside AB DeLeo (1999) ArticleTitleGeneration of anti-p53 cytotoxic T lymphocytes from human peripheral blood using autologous dendritic cells Clin Cancer Res 5 1281–1288 Occurrence Handle1:CAS:528:DyaK1MXkt1Oqurw%3D Occurrence Handle10389910
M Oren W Maltzman AJ Levine (1981) ArticleTitlePosttranslational regulation of the 54K cellular tumor antigen in normal and transformed cells Mol Cell Biol 1 101–110 Occurrence Handle1:CAS:528:DyaL3MXhtF2itrw%3D Occurrence Handle6100960
LG Jial M Osada C Ishioka M Gamo S Iwaki T Suzuki H Shimodaira T Niitani T Kudo M Akiyama N Kimura M Matsuo H Mizusawa N Tanaka H Koyama M Namba R Kanamaru T Kuroki (1997) ArticleTitleStudy of p53 status of cultured cell lines maintained mainly in the JCRB cell bank Mol Carcinogen 19 243–253
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Tsurushima, H., Yoshii, Y., Leong, K.W. et al. Targeted Tumor Cell Death Induced by Autologous Tumor-Specific T Lymphocyte Recognition of Wild-Type p53-Derived Peptides. J Neurooncol 76, 99–104 (2006). https://doi.org/10.1007/s11060-005-4172-6
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DOI: https://doi.org/10.1007/s11060-005-4172-6