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Silver nanoparticles as matrix for laser desorption/ionization mass spectrometry of peptides

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Abstract

Silver nanoparticle synthesized from chemical reduction has been successfully utilized as a matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) of peptides. Acting as a substrate to adsorb analytes, as well as a transmission medium for UV laser, silver nanoparticle was found to assist in the desorption/ionization of peptides with little or no induced fragmentation. The size of the nanoparticle was typically in the range of 160 ± 20 nm. One of the key advantages of silver nanoparticle for peptides analysis is its simple step for on-probe sample preparation. In addition, it also minimizes the interferences of sodium dodecyl sulfate (SDS) surfactant background signal, resulting in cleaner mass spectra and more sensitive signal, when compared to α–cyano-4-hydroxycinnamic acid (CCA) matrix.

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Acknowledgement

This work was supported by Chinese National Natural Science Foundation (Grant No. 90406016).

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Authors and Affiliations

  1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, 321004, P.R. China

    Lin Hua & Jianrong Chen

  2. Natural Sciences and Science Education Academic Group, Nanyang Technological University, Singapore, 637616, Singapore

    Liya Ge & Swee Ngin Tan

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  1. Lin Hua
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  2. Jianrong Chen
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  3. Liya Ge
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Correspondence to Lin Hua.

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Hua, L., Chen, J., Ge, L. et al. Silver nanoparticles as matrix for laser desorption/ionization mass spectrometry of peptides. J Nanopart Res 9, 1133–1138 (2007). https://doi.org/10.1007/s11051-007-9244-4

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  • DOI: https://doi.org/10.1007/s11051-007-9244-4

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