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MMP-2, TNF-α and NLRP1 polymorphisms in Chinese patients with ankylosing spondylitis and rheumatoid arthritis

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Abstract

Rheumatoid arthritis (RA) and ankylosing spondylitis (AS) are autoimmune, inflammatory diseases with substantial genetic contributions. Matrix metalloproteinase (MMP)-2, tumor necrosis factor (TNF)-α and NLR family pyrin domain-containing 1 (NLRP1) play important roles in the immune response. We studied the MMP-2 rs243865 C/T, TNF-α rs1800629 A/G, NLRP1 rs878329 C/G and NLRP1 rs6502867 C/T polymorphisms in a Chinese cohort of 520 patients with RA, 100 with AS and 520 controls. Genotyping was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Using the MMP-2 rs243865 CC homozygote genotype as the reference group, the CT and TT/CT genotypes were associated with significantly reduced risks of AS. However, logistic regression analyses revealed that the MMP-2 rs243865 C/T polymorphism was not associated with risk of RA. TNF-α rs1800629 A/G, NLRP1 rs878329 C/G and NLRP1 rs6502867 C/T polymorphisms were not associated with risk of RA or AS. These findings suggest that the MMP-2 rs243865 C/T polymorphism is associated with AS development.

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Abbreviations

CI:

Confidence interval

LD:

Linkage disequilibrium

MMP-2:

Matrix metalloproteinase-2

NLRP1:

NLR family pyrin domain-containing 1

OR:

Odds ratio

SNP:

Single nucleotide polymorphism

TNF-α:

Tumor necrosis factor-α

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Acknowledgments

This study was supported in part by National Natural Science Foundation of China (81371927) and Nanjing Medical University Foundation for Development of Science and Technology (06NMUZ045, 2012NJMU128).

Conflict of interest

None of the authors has any potential financial conflict of interest related to this manuscript.

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Authors and Affiliations

  1. Department of Orthopedic Trauma, Affiliated Hospital of Nanjing Medical University, Changzhou Second People’s Hospital, Changzhou, 213003, China

    Rongbin Sun, Yong Huang, Hui Zhang & Ruiping Liu

  2. Central Laboratory, Affiliated Hospital of Nanjing Medical University, Changzhou Second People’s Hospital, Changzhou, 213003, China

    Ruiping Liu

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  1. Rongbin Sun
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Correspondence to Ruiping Liu.

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Sun, R., Huang, Y., Zhang, H. et al. MMP-2, TNF-α and NLRP1 polymorphisms in Chinese patients with ankylosing spondylitis and rheumatoid arthritis. Mol Biol Rep 40, 6303–6308 (2013). https://doi.org/10.1007/s11033-013-2743-8

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  • DOI: https://doi.org/10.1007/s11033-013-2743-8

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