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Shine-Dalgarno sequence of bacteriophage T4: GAGG prevails in early genes

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Abstract

Translation initiation is governed by a limited number of mRNA sequence motifs within the translation initiation region (TIR). In bacteria and bacteriophages, one of the most important determinants is a Shine-Dalgarno (SD) sequence that base pairs with the anti-SD sequence GAUCACCUCCUUA localized in the 3′ end of 16S rRNA. This work assesses a diversity of TIR features in phage T4, focusing on the SD sequence, its spacing to the start codon and relationship to gene expression and essentiality patterns. Analysis shows that GAGG is predominant of all core SD motifs in T4 and its related phages, particularly in early genes. Possible implication of the RegB activity is discussed.

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Acknowledgments

The author is grateful to anonymous reviewers for constructive and critical comments. N.M. acknowledges the support of the BBSRC/EPSRC Grant BB/C008219/1 “The Manchester Centre for Integrative Systems Biology (MCISB)”.

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  1. Faculty of Life Sciences, MCISB, MIB, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK

    Naglis Malys

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  1. Naglis Malys
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Correspondence to Naglis Malys.

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Malys, N. Shine-Dalgarno sequence of bacteriophage T4: GAGG prevails in early genes. Mol Biol Rep 39, 33–39 (2012). https://doi.org/10.1007/s11033-011-0707-4

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