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Clustered QTL for source leaf size and yield traits in rice (Oryza sativa L.)

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Abstract

Improvement of plant type plays an important role in super-high yield breeding in rice (Oryza sativa L.). In the present study, a set of backcross recombinant inbred lines derived from a cross of 9311 and Zhenshan97, both elite indica hybrid parents, were developed to identify quantitative trait loci (QTL) for flag leaf size, panicle and yield traits. Forty-seven QTL for 14 traits were detected in common in the two environmental trials, of which nine genomic regions contained clustered QTL affecting plant type traits and yield traits. Four co-localized QTL on chromosomes 1, 6, 7 and 8 involving QTL for flag leaf size (flag leaf length, width and area) contained the QTL for yield traits such as panicle weight (PW) and secondary branch number (SBN), and in all cases alleles from 9311 increased source leaf size and were associated with increased sink size and yield (SBN and PW). Using a subset of overlapping substitution lines for the QTL region on chromosome 1, the QTL were validated and narrowed into a 990 kbp interval (RM3746–RM10435) with pleiotropic effects on flag leaf size, PW, SBN and spikelet number per panicle. These QTL clusters with large effects on source leaf size and yield-related traits provide good targets for marker-assisted breeding for plant type improvement and high-yield potential in rice.

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Acknowledgments

We are grateful to Dr. Sheng Chen (University of Western Australia) for helpful comments on the manuscript. This work was supported in part by a grant from the National Natural Science Foundation of China, and grants from the Ministry of Science and Technology of China (2006AA10Z151) and the Chinese Ministry of Agriculture (2006-G1).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, and The College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China

    Peng Wang, Guilin Zhou, Kehui Cui & Sibin Yu

  2. The College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China

    Zhikang Li

  3. National Key Facility for Crop Gene Resources & Genetic Improvement, Chinese Academy of Agricultural Sciences, 100081, Beijing, China

    Zhikang Li

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  1. Peng Wang
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  2. Guilin Zhou
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  3. Kehui Cui
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  4. Zhikang Li
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  5. Sibin Yu
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Correspondence to Sibin Yu.

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Peng Wang and Guilin Zhou contributed equally to this work.

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Wang, P., Zhou, G., Cui, K. et al. Clustered QTL for source leaf size and yield traits in rice (Oryza sativa L.). Mol Breeding 29, 99–113 (2012). https://doi.org/10.1007/s11032-010-9529-7

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