Skip to main content

Advertisement

SpringerLink
Log in

Search for a diagnostic/prognostic biomarker for the brain cancer glioblastoma multiforme by 2D-DIGE-MS technique

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

The prognosis of patients with glioblastoma multiforme, the most malignant adult glial brain tumor, remains poor in spite of advances in treatment procedures, including surgical resection, irradiation, and chemotherapy. Genetic heterogeneity of glioblastoma warrants extensive studies to gain a thorough understanding of the biology of this tumor. While there have been several studies of global transcript profiling of glioma with the identification of gene signatures for diagnosis and disease management, translation into clinics is yet to happen. In the present study, we report a novel proteomic approach by using two-dimensional difference gel electrophoresis followed by spot picking and analysis of proteins/peptides by Mass spectrometry. We report at least ten different novel proteins/peptides as identified by this technique which are differentially expressed in this cancer and could be of further importance for diagnostic, therapeutic, and prognostic approaches.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  1. Burger PC, Scheithauer BW (1994) Tumors of the central nervous system (atlas of the tumor pathology), vol 3(10). Armed Forces Institute of Pathology, Washington, DC

    Google Scholar 

  2. Ramsey AJ, Milenkovic M, Oliveira AF, Escobedo-Lozoya Y, Seshadri S, Salahpour A, Sawa A, Yasuda R, Caron MG (2011) Impaired NMDA receptor transmission alters striatal synapses and DISC1 protein in an age-dependent manner. Proc Natl Acad Sci USA 108(14):5795–5800

    Article  PubMed  CAS  Google Scholar 

  3. Huang Z, Hoffmann FKW, Norton RL, Hashimoto AC, Hoffmann PR (2011) Selenoprotein k is a novel target of m-calpain and cleavage is regulated by toll-like receptor-induced calpastatin in macrophages. J Biol Chem 286:34830–34838

    Article  PubMed  CAS  Google Scholar 

  4. Froman DP, Feltmann AJ, Pendarvis K, Cooksey AM, Burgess SC, Rhoads DD (2011) A proteome-based model for sperm mobility phenotype. J Anim Sci 89:1330–1337

    Article  PubMed  CAS  Google Scholar 

  5. Ünlü M, Morgan ME, Minden JS (1997) Difference gel electrophoresis: a single gel method for detecting changes in protein extracts. Electrophoresis 18:2071–2077

    Article  PubMed  Google Scholar 

  6. Hill D, Coss C, Dubey JP, Wroblewski K, Sautter M, Hosten T, Munoz-Zanzi C, Mui E, Withers S, Boyer K, Hermes G, Coyne J, Jagdis F, Burnett A, McLeod P, Morton H, Robinson D, McLeod R, Toxoplasmosis Study Group (2011) Identification of a sporozoite-specific antigen from Toxoplasma gondii. J Parasitol 97:328–337

    Article  PubMed  CAS  Google Scholar 

  7. Huang NF, Kurpinski K, Qizhi Fang QZ, Lee RJ, Li S (2011) Proteomic identification of biomarkers of vascular injury. Am J Transl Res 3:139–148

    PubMed  CAS  Google Scholar 

  8. Marshall JL, Huestis DL, Garcia C, Hiromasa Y, Wheeler S, Noh S, Tomich JM, Howard DJ (2011) Comparative proteomics uncovers the signature of natural selection acting on the ejaculate proteomes of two cricket species isolated by postmating, prezygotic phenotypes. Mol Biol Evol 28:423–435

    Article  PubMed  CAS  Google Scholar 

  9. Kirpich IA, Gobejishvili LN, Homme MB, Waigel S, Cave M, Arteel G, Barve SS, McClain CJ, Deaciuc IV (2011) Integrated hepatic transcriptome and proteome analysis of mice with high-fat diet-induced nonalcoholic fatty liver disease. J Nutr Biochem 22:38–45

    Article  PubMed  CAS  Google Scholar 

  10. Thakkar D, Shervington L, Shervington A (2011) Proteomic studies coupled with RNAi methodologies can shed further light on the downstream effects of telomerase in glioma. Cancer Investig 29:113–122

    Article  CAS  Google Scholar 

  11. Saraswathy S, Rao NA (2011) Posttranslational modification of differentially expressed mitochondrial proteins in the retina during early experimental autoimmune uveitis. Mol Vis 17:1814–1821

    PubMed  CAS  Google Scholar 

  12. Ji C, Kaplowitz N, Lau MY, Kao E, Petrovic LM, Lee AS (2011) Liver-specific loss of GRP78 perturbs globally unfolded protein response and exacerbates a spectrum of acute and chronic liver diseases. Hepatology 54:229–239

    Article  PubMed  CAS  Google Scholar 

  13. Oggé G, Romero R, Lee DC, Gotsch F, Than NG, Lee J, Chaiworapongsa T, Dong Z, Mittal P, Hassan SS, Kim CJ (2011) Chronic chorioamnionitis displays distinct alterations of the amniotic fluid proteome. J Pathol 223(4):553–565

    Article  PubMed  Google Scholar 

  14. Hopf PS, Ford RS, Zebian N, Merkx-Jacques A, Vijayakumar S, Ratnayake D, Hayworth J, Creuzenet C (2011) Protein glycosylation in Helicobacter pylori: beyond the flagellins? PLoS ONE 6(9):e25722. doi:10.1371/journal.pone.0025722

    Article  PubMed  CAS  Google Scholar 

  15. Bryant KG, Camacho J, Jasmin JF, Wang CG, Addya A, Casimiro MC, Fortina P, Balasubramaniam S, Knudsen KE, Schwarting R, Lisanti MP, Mercier I (2011) Caveolin-1 overexpression enhances androgen-dependent growth and proliferation in the mouse prostate. Int J Biochem Cell Biol 43:1318–1329

    Article  PubMed  CAS  Google Scholar 

  16. Boone LR, Lagor WR, de la Llera Moya M, Niesen MI, Rothblat GH, Ness GC (2011) Thyroid hormone enhances the ability of serum to accept cellular cholesterol via the ABCA1 transporter. Atherosclerosis 218:77–82

    Article  PubMed  CAS  Google Scholar 

  17. Xi L, Zhu SG, Hobbs DC, Kukreja RC (2011) Identification of protein targets underlying dietary nitrate-induced protection against doxorubicin ardiotoxicity. J Cell Mol Med 15:2512–2524

    Article  PubMed  CAS  Google Scholar 

  18. Trimmer C, Sotgia F, Whitaker-Menezes D, Balliet RM, Eaton G, Martinez-Outschoorn UE, Pavlides S, Howell A, Iozzo RV, Pestell RG, Scherer PE, Capozza F, Lisanti MP (2011) Caveolin-1 and mitochondrial SOD2 (MnSOD) function as tumor suppressors in the stromal microenvironment. Cancer Biol Ther 11:383–394

    Article  PubMed  CAS  Google Scholar 

  19. Arce-Paredes P, Mora-Escobedo R, Luna-Arias JP, Mendoza-Hernández G, Rojas-Espinosa O (2011) Heat, salinity, and acidity, commonly upregulate A1aB1b proglycinin in soybean embryonic axes. In: Ng TB (ed) Soybean—biochemistry, chemistry and physiology. InTech (open access), Rijeka, pp 402–422

  20. Green KN, Khashwji H, Estrada T, Laferla FM (2011) ST101 induces a novel 17kDa APP cleavage that precludes Aβ generation in vivo. Ann Neurol 69(5):831–844

    Article  PubMed  CAS  Google Scholar 

  21. Balestrieri ML, Giovane A, Milone L, Felice F, Fiorito C, Crudele V, Esposito A, Rossiello R, Minucci PB, Farzati B, Servillo L, Napoli C (2010) Modification of the detrimental effect of TNF-α on human endothelial progenitor cells by fasudil and Y27632. J Biochem Mol Toxicol 24:351–360

    Article  PubMed  CAS  Google Scholar 

  22. Kongara S, Kravchuk O, Teplova I, Lozy F, Schulte J, Moore D, Barnard N, Neumann CA, White E, Karantza V (2010) Autophagy regulates keratin 8 homeostasis in mammary epithelial cells and in breast tumors. Mol Cancer Res 8(6):873–884

    Article  PubMed  CAS  Google Scholar 

  23. Zhang HH, Feng L, Livnat I, Hoh JK, Shim JY, Liao WX, Chen DB (2010) Estradiol-17(beta) stimulates specific receptor and endogenous nitric oxide-dependent dynamic endothelial protein S-nitrosylation: analysis of endothelial nitrosyl-proteome. Endocrinology 151:3874–3887

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Authors are grateful to Dr. John Liao of Applied Biomics, CA, USA, for his help; Supported by US DOE/TMCF grant to Dr. H. Banerjee and a US DOE graduate student training grant to Elizabeth City State University graduate school.

Author information

Authors and Affiliations

  1. Department of Biological and Pharmaceutical Sciences, Elizabeth City State University, University of North Carolina, Elizabeth City, NC, 27909, USA

    Hirendra Nath Banerjee, Kelly Mahaffey, Eilena Riddick & Arnold Banerjee

  2. Department of Chemistry, University of Nevada Las Vegas, 4505 S Maryland Parkway, Las Vegas, NV, 89154, USA

    Niladri Bhowmik & Manomita Patra

Authors
  1. Hirendra Nath Banerjee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kelly Mahaffey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eilena Riddick
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Arnold Banerjee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Niladri Bhowmik
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Manomita Patra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hirendra Nath Banerjee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Banerjee, H.N., Mahaffey, K., Riddick, E. et al. Search for a diagnostic/prognostic biomarker for the brain cancer glioblastoma multiforme by 2D-DIGE-MS technique. Mol Cell Biochem 367, 59–63 (2012). https://doi.org/10.1007/s11010-012-1319-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11010-012-1319-6

Keywords

Search

Navigation