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Metal Enhanced Fluorescence Solution-based Sensing Platform 2: Fluorescent Core-Shell Ag@SiO2 Nanoballs

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Abstract

In this Rapid Communication, we present the development of monodisperse core-shell (silver core-silica shell) nanoparticles with various shell thicknesses featuring a fluorophore, subsequently named Metal-Enhanced Fluorescence (MEF) nanoballs. MEF nanoballs consist of a ≈130 nm silver nanoparticle core, a silica shell with up to 35 nm thickness and fluorophores doped within the silica shell. Fluorescent nanobubbles where the silver core is removed by chemical etching are used as control samples to show the benefits of using silver nanoparticles, i.e, Metal-Enhanced Fluorescence. Finally, we demonstrate the broad potential biological applications of MEF nanoballs by employing near-infra red emitting probes (Rhodamine 800) within the silica shell, for potential applications in cellular imaging and solution-based sensing.

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Fig. 1
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Abbreviations

Ag@SiO2 :

Silver core, silica shell nanoparticles

MEF:

Metal-Enhanced Fluorescence

SPR:

Surface Plasmon Resonance

Rh800:

Rhodamine 800

TEM:

Transmission Electron Microscope

TEOS:

Tetraethyl orthosilicate

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Acknowledgments

This work was partially supported by the National Center for Research Resources, RR008119. Salary support to KA and CDG from UMBI is also acknowledged. The authors would like to thank Dr. J. Zhang for his help with the TEM images.

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Authors and Affiliations

  1. Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 W. Lombard St., Baltimore, MD, 21201, USA

    Kadir Aslan & Chris D. Geddes

  2. Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 W. Lombard St., Baltimore, MD, 21021, USA

    Meng Wu, Joseph R. Lakowicz & Chris D. Geddes

Authors
  1. Kadir Aslan
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  2. Meng Wu
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  3. Joseph R. Lakowicz
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  4. Chris D. Geddes
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Corresponding author

Correspondence to Chris D. Geddes.

Additional information

Kadir Aslan, Meng Wu, Contributed equally

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Aslan, K., Wu, M., Lakowicz, J.R. et al. Metal Enhanced Fluorescence Solution-based Sensing Platform 2: Fluorescent Core-Shell Ag@SiO2 Nanoballs. J Fluoresc 17, 127–131 (2007). https://doi.org/10.1007/s10895-007-0164-6

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  • DOI: https://doi.org/10.1007/s10895-007-0164-6

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