Abstract
Increasing global atmospheric CO2 has been shown to affect important plant traits, including constitutive levels of defensive compounds. However, little is known about the effects of elevated CO2 on the inducibility of chemical defenses or on plant mechanical defenses. We grew Brassica rapa (oilseed rape) under ambient and elevated CO2 to determine the effects of elevated CO2 on constitutive levels and inducibility of carbon-based phenolic compounds, and on constitutive trichome densities. Trichome density increased by 57% under elevated CO2. Constitutive levels of simple, complex, and total phenolics also increased under elevated CO2, but inducibility of each decreased. Induction of simple phenolics occurred only under ambient CO2. Although induction of complex and total phenolics occurred under both ambient and elevated CO2, the damage-induced increases were 64% and 75% smaller, respectively, under elevated CO2. Constitutive phenolic levels were positively correlated with leaf C:N ratio, and inducibility was positively correlated with leaf N and negatively correlated with leaf C:N ratio, as would be expected if inducibility were constrained by nitrogen availability under elevated CO2. We conclude that B. rapa is likely to exhibit higher constitutive levels of both chemical and mechanical defenses in the future, but is also likely to be less able to respond to herbivore damage by inducing phenolic defenses. To our knowledge, this is only the second study to report a negative effect of elevated CO2 on the inducibility of any plant defense.
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Acknowledgements
We thank Mike Grant for assistance with phenolic assays, John Landosky for providing plants, Justin Knight for assisting with trichome counts, the University of Michigan Biological Station for logistical support, and two anonymous reviewers for helpful comments. This work was supported by National Science Foundation grant DEB-9796250 and United States Department of Agriculture grant NRICGP-9706410 to D. Karowe, and National Science Foundation Research Experience for Undergraduates grant DBI-9731615 to J. Teeri and D. Karowe.
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Karowe, D.N., Grubb, C. Elevated CO2 Increases Constitutive Phenolics and Trichomes, but Decreases Inducibility of Phenolics in Brassica rapa (Brassicaceae). J Chem Ecol 37, 1332–1340 (2011). https://doi.org/10.1007/s10886-011-0044-z
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DOI: https://doi.org/10.1007/s10886-011-0044-z