Skip to main content

Advertisement

SpringerLink
Log in

Influence of Maternal Hyperglycemia on IL-10 and TNF-α Production: The Relationship with Perinatal Outcomes

  • Published:
Journal of Clinical Immunology Aims and scope Submit manuscript

Abstract

Aims

This study was conducted to evaluate maternal and placental concentrations of interleukin 10 (IL-10) and tumor necrosis factor-alpha (TNF-α) in pregnant women with glycemic mean (GM) < or ≥100 mg/dL, as well as correlate IL-10 and TNF-α placental concentrations with perinatal outcomes.

Methods

One hundred eighty-six pregnant women were distributed in groups determined by a GM <100 mg/dL or a GM ≥100 mg/dL. The GM, HbA1c levels, maternal and placental concentrations of IL-10 and TNF-α, and the correlation of placental cytokines with perinatal outcomes were evaluated.

Results

In maternal blood, the lowest concentrations of IL-10 (p = 0.0019) and TNF-α (p = 0.0185) were observed in the GM ≥100-mg/dL group. The placentas from GM ≥100 mg/dL group exhibited higher TNF-α concentrations (p = 0.0385). Placental IL-10 directly correlated with hemoglobin (r = 0.63; p = 0.02) and insulin (r = 0.78; p = 0.01) levels in the umbilical cord and with 1-min (r = 0.53; p = 0.0095) and 5-min (r = 0.69; p = 0.0003) Apgar scores. Placental TNF-α displayed a tendency to inversely correlate with fetal weight (r = −0.41; p = 0.05).

Conclusion

Compared to GM <100 mg/dL, GM ≥100 mg/dL was associated with a reduction in maternal IL-10 and TNF-α concentrations and increased placental TNF-α production. Placental IL-10 production was similar in both groups studied and directly correlated with hemoglobin and umbilical cord insulin levels, as well as with the 1- and 5-min Apgar scores.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  1. American Diabetes Association (ADA). Diagnosis and classification of diabetes mellitus. Diabetes Care. 2011;34 Suppl 1:S62–9.

    Article  Google Scholar 

  2. Rudge MV, Calderon IM, Ramos MD, Abbade JF, Rugolo LM. Perinatal outcome of pregnancies complicated by diabetes and by maternal daily hyperglycemia not related to diabetes. A retrospective 10-year analysis. Gynecol Obstet Invest. 2000;50:108–12.

    Article  PubMed  CAS  Google Scholar 

  3. Eriksson UJ, Cederberg J, Wentzel P. Congenital malformations in offspring of diabetic mothers—animal and human studies. Rev Endocr Metab Disord. 2003;4:79–93.

    Article  PubMed  CAS  Google Scholar 

  4. Rudge MVC, Calderon IMP, Ramos MD, Brasil MAM, Rugolo LMSS, Bossolan G, et al. Hiperglicemia materna diária diagnosticada pelo perfil glicêmico: um problema de saúde pública materno e perinatal. Rev Bras Ginecol Obstet. 2005;27:691–7.

    Google Scholar 

  5. Yang J, Cummings EA, O’Connell C, Jangaard K. Fetal and neonatal outcomes of diabetic pregnancies. Obstet Gynecol. 2006;108:644–50.

    Article  PubMed  Google Scholar 

  6. Calderon IMP, Kerche LTRL, Damasceno DC, Rudge MVC. Diabetes and pregnancy: an update of the problem. ARBS. 2007;9:1–11.

    CAS  Google Scholar 

  7. Macintosh MC, Fleming KM, Bailey JA, Doyle P, Modder J, Acolet D, et al. Perinatal mortality and congenital anomalies in babies of women with type 1 or type 2 diabetes in England, Wales, and Northern Ireland: population based study. BMJ. 2006;333:1–6.

    Article  Google Scholar 

  8. Hod M, Haroush AB, Yogev Y. Pathogenesis of gestational diabetes mellitus. In: Hod M, Jovanovic L, Leiva GCRA, Langer O, editors. Textbook of diabetes and pregnancy. London: Informa Healthcare; 2003. p. 39–49.

    Google Scholar 

  9. Vela-Huerta MM, San Vicente-Santoscoy EU, Guizar-Mendoza JM, Amador-Licona N, Aldana-Valenzuela C, Hernnandez J. Leptin, insulin, and glucose serum levels in large-for-gestational-age infants of diabetic and non-diabetic mothers. J Pediatr Endocrinol Metab. 2008;21:17–22.

    Article  PubMed  CAS  Google Scholar 

  10. International Association of Diabetes and Pregnancy Study Groups Consensus Panel. International Association of Diabetes and Pregnancy Study Groups recommendations on the diagnosis and classification of hyperglycemia in pregnancy. Diabetes Care. 2010;33:676–82.

    Article  Google Scholar 

  11. Metzger E, Lowe LP, Dyer AR, Trimble ER, Chaovarindr U, Coustan DR, et al. Hyperglycemia and adverse pregnancy outcomes. The HAPO Study Cooperative Research Group. N Engl J Med. 2008;358:1991–2002.

    Article  PubMed  Google Scholar 

  12. Rudge MVC, Peraçoli JC, Berezowski AT, Calderon IMP, Brasil MAM. The oral glucose tolerance test is a poor predictor of hyperglycemia during pregnancy. Braz J Med Biol Res. 1990;23:1079–89.

    PubMed  CAS  Google Scholar 

  13. Bjork O, Persson B. Villous structure in different parts of the cotyledon in placentas of insulin-dependent diabetic women. A morphometric study. Acta Obstet Gynecol Scand. 1984;63:37–43.

    Article  PubMed  CAS  Google Scholar 

  14. Taricco E, Radaelli T, Nobile de Santis MS, Cetin I. Foetal and placental weights in relation to maternal characteristics in gestational diabetes. Placenta. 2003;24:343–7.

    Article  PubMed  CAS  Google Scholar 

  15. Calderon IM, Damasceno DC, Amorin RL, Costa RA, Brasil MA, Rudge MV. Morphometric study of placental villi and vessels in women with mild hyperglycemia or gestational or overt diabetes. Diabetes Res Clin Pract. 2007;78:65–71.

    Article  PubMed  Google Scholar 

  16. Sgarbosa F, Barbisan LF, Brasil MAM, Costa E, Calderon IMP, Gonçalves CR, et al. Changes in apoptosis and Bcl-2 expression in human hyperglycemic, term placental trophoblast. Diabetes Res Clin Pract. 2006;73:143–9.

    Article  PubMed  CAS  Google Scholar 

  17. Pietro L, Daher S, Rudge MV, Calderon IM, Damasceno DC, Sinzato YK, et al. Vascular endothelial growth factor (VEGF) and VEGF-receptor expression in placenta of hyperglycemic pregnant women. Placenta. 2010;3:7707–80.

    Google Scholar 

  18. Carvalho Silva SAL. Dopplervelocimetria da artéria umbilical como marcador de alterações imunológicas placentárias e de risco de morte perinatal em gestações complicadas por diabete ou hiperglicemia leve [tese]. Faculdade de medicina (Botucatu): Universidade Estadual Paulista; 2010.

  19. Thaxton JE, Sharma S. Interleukin-10: a multi-faceted agent of pregnancy. Am J Reprod Immunol. 2010;63:482–91.

    Article  PubMed  CAS  Google Scholar 

  20. Coughlan MT, Oliva K, Georgiou HM, Permezel JM, Rice GE. Glucose-induced release of tumour necrosis factor-alpha from human placental and adipose tissues in gestational diabetes mellitus. Diabet Med. 2001;18:921–7.

    Article  PubMed  CAS  Google Scholar 

  21. Kirwan JP, Hauguel-De Mouzon S, Lepercq J, Challier JC, Huston-Presley L, Friedman JE, Kalhan SC, Catalano PM. TNF-alpha is a predictor of insulin resistance in human pregnancy. Diabetes. 2002;51:2207–13.

    Article  PubMed  CAS  Google Scholar 

  22. Mosmann TR, Coffman RL. Heterogeneity of cytokine secretion patterns and functions of helper T cells. Adv Immunol. 1989;46:111–47.

    Article  PubMed  CAS  Google Scholar 

  23. Negrato CA, Jovanovic L, Tambascia MA, Calderon Ide M, Geloneze B, Dias A, et al. Mild gestational hyperglycaemia as a risk factor for metabolic syndrome in pregnancy and adverse perinatal outcomes. Diabetes Metab Res Rev. 2008;24:324–30.

    Article  PubMed  Google Scholar 

  24. Rudge MVC, Calderon IMP, Ramos MD, Maestá I, Souza LMS, Peraçoli JC. Perspectiva perinatal decorrentes do rígido controle pré-natal em gestações complicadas pelodiabete. Rev Bras Ginecol Obstet. 1995;17:26–32.

    Google Scholar 

  25. Moller DE. Potential role of TNF-alpha in the pathogenesis of insulin resistance and type 2 diabetes. Trends Endocrinol Metab. 2000;11:212–7.

    Article  PubMed  CAS  Google Scholar 

  26. Montazeri S, Nalliah S, Radhakrishnan AK. Is there a genetic variation association in the IL-10 and TNF alpha promoter gene with gestational diabetes mellitus? Hereditas. 2010;147:94–102.

    Article  PubMed  Google Scholar 

  27. Desoye G, Hauguel-de Mouzon S. The human placenta in gestational diabetes mellitus. The insulin and cytokine network. Diabetes Care. 2007;30:S121–6.

    Article  Google Scholar 

Download references

Acknowledgments

The authors thank the São Paulo Research Foundation (Fundação de Amparo à Pesquisa do Estado de São Paulo—FAPESP, grant number 2007/0071-6 and 2009/03253-1) for financial support and the Research Support Group (Grupo de Apoio a Pesquisa—GAP) of Botucatu Medical School/UNESP for their technical support in statistical analysis.

Conflict of Interest

There are no conflicts of interest.

Author information

Authors and Affiliations

  1. Post-graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, UNESP—São Paulo State University, Botucatu, Brazil

    Jusciele Brogin Moreli, Glilciane Morceli, Débora Cristina Damasceno, Marilza Vieira Cunha Rudge & Iracema Mattos Paranhos Calderon

  2. Department of Obstetrics and Gynecology, Botucatu Medical School, UNESP—São Paulo State University, Distrito de Rubião Jr. s/n, CEP 18618-000, Botucatu, São Paulo, Brazil

    Ana Karina C. De Luca, Claudia G. Magalhães, Roberto A. A. Costa, Débora Cristina Damasceno, Marilza Vieira Cunha Rudge & Iracema Mattos Paranhos Calderon

Authors
  1. Jusciele Brogin Moreli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Glilciane Morceli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ana Karina C. De Luca
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Claudia G. Magalhães
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Roberto A. A. Costa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Débora Cristina Damasceno
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Marilza Vieira Cunha Rudge
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Iracema Mattos Paranhos Calderon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Iracema Mattos Paranhos Calderon.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moreli, J.B., Morceli, G., De Luca, A.K.C. et al. Influence of Maternal Hyperglycemia on IL-10 and TNF-α Production: The Relationship with Perinatal Outcomes. J Clin Immunol 32, 604–610 (2012). https://doi.org/10.1007/s10875-011-9634-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10875-011-9634-3

Keywords

Search

Navigation