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Joint composite-rotation adiabatic-sweep isotope filtration

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Abstract

Joint composite-rotation adiabatic-sweep isotope filters are derived by combining the composite-rotation [Stuart AC et al. (1999) J Am Chem Soc 121: 5346–5347] and adiabatic-sweep [Zwahlen C et al. (1997) J Am Chem Soc 119:6711–6721; Kupče E, Freeman R (1997) J Magn Reson 127:36–48] approaches. The joint isotope filters have improved broadband filtration performance, even for extreme values of the one-bond 1H–13C scalar coupling constants in proteins and RNA molecules. An average Hamiltonian analysis is used to describe evolution of the heteronuclear scalar coupling interaction during the adiabatic sweeps within the isotope filter sequences. The new isotope filter elements permit improved selective detection of NMR resonance signals originating from 1H spins attached to an unlabeled natural abundance component of a complex in which the other components are labeled with 13C and 15N isotopes.

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Acknowledgements

This work was supported by NIH grants GM50291 (A.G. P.) and GM47021 (D. C.). E.R.V. acknowledges support from a National Science Foundation Graduate Research Fellowship. We thank Joel A. Butterwick (Columbia University), Thomas A. Edwards (Mt. Sinai School of Medicine), and Aneel K. Aggarwal (Mt. Sinai School of Medicine) for the Vts1 SAM domain/TCE 13mer RNA sample. Helpful discussions with Mark Rance (Univ. Cincinnati) are acknowledged gratefully.

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  1. Fabien Ferrage

    Present address: Département de Chimie, CNRS UMR 8642 École normale supérieure, 24 rue Lhomond, 75231, Paris Cedex 5, France

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY, 10032, USA

    Elizabeth R. Valentine, Francesca Massi & Arthur G. Palmer III

  2. New York Structural Biology Center, 89 Convent Avenue, New York, NY, 10027-7556, USA

    Fabien Ferrage & David Cowburn

Authors
  1. Elizabeth R. Valentine
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  2. Fabien Ferrage
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  3. Francesca Massi
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  4. David Cowburn
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  5. Arthur G. Palmer III
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Correspondence to Arthur G. Palmer III.

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Valentine, E.R., Ferrage, F., Massi, F. et al. Joint composite-rotation adiabatic-sweep isotope filtration. J Biomol NMR 38, 11–22 (2007). https://doi.org/10.1007/s10858-006-9131-9

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  • DOI: https://doi.org/10.1007/s10858-006-9131-9

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