Abstract
A bone inspired material was obtained by incorporating collagen in the liquid phase of an α-tricalcium phosphate cement, either in solubilized or in fibrilized form. This material was able to set in situ, giving rise to a calcium deficient hydroxyapatite (CDHA)/collagen composite. The morphology and distribution of collagen in the composite was shown to be strongly affected by the collagen pre-treatment. The interactions between collagen and the inorganic phase were assessed by FTIR. A red shift of the amide I band was indicative of calcium chelation by the collagen carbonyl groups. The rate of CDHA formation was not affected when diluted collagen solutions (1 mg/ml) were used, whereas injectability improved. The presence of solubilized collagen, even in low amount (1 %), increased cell adhesion and proliferation on the composites. Still in the absence of osteogenic medium, significant ALP activity was detected both in the inorganic and the collagen-containing cements. The maximum ALP activity was advanced in the collagen-containing cement as compared to the inorganic cement.
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Acknowledgments
The authors thank Eduardo Jorge-Herrero at Clínica Puerta de Hierro in Madrid for kindly donating the collagen for this work, and F. Puig for his technical support. This study was supported by the Spanish Ministry of Science and Education through project MAT2009-13547. Support for MPG was also received though the prize “ICREA Academia” for excellence in research, funded by the Generalitat de Catalunya.
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Perez, R.A., Ginebra, MP. Injectable collagen/α-tricalcium phosphate cement: collagen–mineral phase interactions and cell response. J Mater Sci: Mater Med 24, 381–393 (2013). https://doi.org/10.1007/s10856-012-4799-8
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DOI: https://doi.org/10.1007/s10856-012-4799-8