Skip to main content

Advertisement

SpringerLink
Log in

Characterization of an antimicrobial dental resin adhesive containing zinc methacrylate

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

This study evaluates the effect of zinc methacrylate (ZM) on the degree of conversion (DC), cytotoxicity and antimicrobial activity (AA) of an experimental resin. Tetraethyleneglycol dimethacrylate was used as the experimental resin and it was photo activated using camphoroquinone and ethyl 4-dimethylamine benzoate. Additionally, 1.0, 2.5, 5.0, 10, 20 and 30 wt% of ZM was added to the various experimental resins. The DC was accessed by Fourier Transform infrared spectroscopy. For cytotoxicity, immortalized mouse fibroblasts were exposed to the experimental resin extracts. An MTT assay was used to access the cytotoxicity. The AA against Streptococcus mutans UA159 was accessed by the agar diffusion method. An improvement in the DC in all concentrations of ZM was observed. The greater the amount of ZM on the experimental resin, the less the cytotoxicity was provoked. Three ZM concentrations showed AA that exhibited various inhibition growth zones with 10, 20 (10 mm) and 30 wt% (15 mm).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. De Munck J, Van Landuyt K, Peumans M, Poitevin A, Lambrechts P, Braem M, Van Meerbeek B. A critical review of the durability of adhesion to tooth tissue: methods and results. J Dent Res. 2005;84:118–32.

    Article  Google Scholar 

  2. Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De Stefano Dorigo E. Dental adhesion review: aging and stability of the bonded interface. Dent Mater. 2008;24:90–101.

    Article  CAS  Google Scholar 

  3. Abdalla AI, Feilzer AJ. Four-year water degradation of a total-etch and two self-etching adhesives bonded to dentin. J Dent. 2008;36:611–7.

    Article  CAS  Google Scholar 

  4. De Munck J, Van Meerbeek B, Yoshida Y, Inoue S, Vargas M, Suzuki K, Lambrechts P, Vanherle G. Four-year water degradation of total-etch adhesives bonded to dentin. J Dent Res. 2003;82:136–40.

    Article  Google Scholar 

  5. Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S. Collagen degradation by host-derived enzymes during aging. J Dent Res. 2004;83:216–21.

    Article  CAS  Google Scholar 

  6. Fontes ST, Ogliari FA, Lima GS, Bueno M, Schneider LF, Piva E. Tetrahydrofuran as alternative solvent in dental adhesive systems. Dent Mater. 2009;25:1503–8.

    Article  CAS  Google Scholar 

  7. Zanchi CH, Munchow EA, Ogliari FA, Chersoni S, Prati C, Demarco FF. Piva E development of experimental HEMA-free three-step adhesive system. J Dent. 2010;38:503–8.

    Article  CAS  Google Scholar 

  8. Ogliari FA, Ely C, Petzhold CL, Demarco FF, Piva E. Onium salt improves the polymerization kinetics in an experimental dental adhesive resin. J Dent. 2007;35:583–7.

    Article  CAS  Google Scholar 

  9. Carrilho MR, Carvalho RM, de Goes MF, di Hipolito V, Geraldeli S, Tay FR, Pashley DH, Tjaderhane L. Chlorhexidine preserves dentin bond in vitro. J Dent Res. 2007;86:90–4.

    Article  CAS  Google Scholar 

  10. Carrilho MR, Geraldeli S, Tay F, de Goes MF, Carvalho RM, Tjaderhane L, Reis AF, Hebling J, Mazzoni A, Breschi L, Pashley D. In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res. 2007;86:529–33.

    Article  CAS  Google Scholar 

  11. Hebling J, Pashley DH, Tjaderhane L, Tay FR. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res. 2005;84:741–6.

    Article  CAS  Google Scholar 

  12. de Souza AP, Gerlach RF, Line SR. Inhibition of human gingival gelatinases (MMP-2 and MMP-9) by metal salts. Dent Mater. 2000;16:103–8.

    Article  Google Scholar 

  13. Santos MC, de Souza AP, Gerlach RF, Trevilatto PC, Scarel-Caminaga RM, Line SR. Inhibition of human pulpal gelatinases (MMP-2 and MMP-9) by zinc oxide cements. J Oral Rehabil. 2004;31:660–4.

    Article  CAS  Google Scholar 

  14. Santos MC, Souza AP, Gerlach RF, Tabchoury CM, Line SR. Inhibition of human gelatinases (matrix metalloproteinase-2 and matrix metalloproteinase-9) activity by zinc oxide: a possible mechanism to enhance wound healing. Br J Dermatol. 2001;145:854–5.

    Article  CAS  Google Scholar 

  15. Souza AP, Gerlach RF, Line SR. Inhibition of human gelatinases by metals released from dental amalgam. Biomaterials. 2001;22:2025–30.

    Article  CAS  Google Scholar 

  16. Henn S, de Carvalho RV, Ogliari FA, de Souza AP, Line SR, da Silva AF, Demarco FF, Etges A, Piva E. Addition of zinc methacrylate in dental polymers: MMP-2 inhibition and ultimate tensile strength evaluation. Clin Oral Investig. 2011. doi:10.1007/s00784-011-0551-x.

  17. Manhart J, Chen H, Hamm G, Hickel R. Buonocore memorial lecture. Review of the clinical survival of direct and indirect restorations in posterior teeth of the permanent dentition. Oper Dent. 2004;29:481–508.

    Google Scholar 

  18. Zhou J, Tan J, Chen L, Li D, Tan Y. The incorporation of chlorhexidine in a two-step self-etching adhesive preserves dentin bond in vitro. J Dent. 2009;37:807–12.

    Article  CAS  Google Scholar 

  19. Wiegand A, Buchalla W, Attin T. Review on fluoride-releasing restorative materials—fluoride release and uptake characteristics, antibacterial activity and influence on caries formation. Dent Mater. 2007;23:343–62.

    Article  CAS  Google Scholar 

  20. Stanislawczuk R, Amaral RC, Zander-Grande C, Gagler D, Reis A, Loguercio AD. Chlorhexidine-containing acid conditioner preserves the longevity of resin-dentin bonds. Oper Dent. 2009;34:481–90.

    Article  CAS  Google Scholar 

  21. Spencer CG, Campbell PM, Buschang PH, Cai J, Honeyman AL. Antimicrobial effects of zinc oxide in an orthodontic bonding agent. Angle Orthod. 2009;79:317–22.

    Article  Google Scholar 

  22. Marczuk-Kolada G, Jakoniuk P, Mystkowska J, Luczaj-Cepowicz E, Waszkiel D, Dabrowski JR, Leszczynska K. Fluoride release and antibacterial activity of selected dental materials. Postepy Hig Med Dosw. 2006;60:416–20.

    Google Scholar 

  23. Niu LN, Fang M, Jiao K, Tang LH, Xiao YH, Shen LJ, Chen JH. Tetrapod-like zinc oxide whisker enhancement of resin composite. J Dent Res. 2010;89:746–50.

    Article  CAS  Google Scholar 

  24. Gioka C, Bourauel C, Hiskia A, Kletsas D, Eliades T, Eliades G. Light-cured or chemically cured orthodontic adhesive resins? A selection based on the degree of cure, monomer leaching, and cytotoxicity. Am J Orthod Dentofac Orthop. 2005;127:413–9.

    Article  Google Scholar 

  25. Emmler J, Seiss M, Kreppel H, Reichl FX, Hickel R, Kehe K. Cytotoxicity of the dental composite component TEGDMA and selected metabolic by-products in human pulmonary cells. Dent Mater. 2008;24:1670–5.

    Article  CAS  Google Scholar 

  26. Darmani H, Al-Hiyasat AS, Milhem MM. Cytotoxicity of dental composites and their leached components. Quintessence Int. 2007;38:789–95.

    Google Scholar 

  27. Jacobsen JA, Major Jourden JL, Miller MT, Cohen SM. To bind zinc or not to bind zinc: an examination of innovative approaches to improved metalloproteinase inhibition. Biochim Biophys Acta. 2010;1803:72–94.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors were grateful to CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for the scholarship and to Esstech, Inc. for the donation of the reagents and CNPq for financial support (Grant 306822/2008-3).

Author information

Authors and Affiliations

  1. Department of Operative Dentistry, CDC-Bio, School of Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves, 457, Pelotas, RS, 96015-560, Brazil

    Sandrina Henn, Fernanda Nedel, Rafael Guerra Lund, Maximiliano Sérgio Cenci, Tatiana Pereira-Cenci, Flávio Fernando Demarco & Evandro Piva

  2. Department of Restorative Dentistry, School of Dentistry, University of North Parana, Londrina, PR, Brazil

    Rodrigo Varella de Carvalho

Authors
  1. Sandrina Henn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fernanda Nedel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rodrigo Varella de Carvalho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rafael Guerra Lund
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maximiliano Sérgio Cenci
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tatiana Pereira-Cenci
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Flávio Fernando Demarco
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Evandro Piva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Evandro Piva.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Henn, S., Nedel, F., de Carvalho, R.V. et al. Characterization of an antimicrobial dental resin adhesive containing zinc methacrylate. J Mater Sci: Mater Med 22, 1797–1802 (2011). https://doi.org/10.1007/s10856-011-4364-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-011-4364-x

Keywords

Search

Navigation