Abstract
Different cues present in the cellular environment control basic biological processes. A previously established 3D microwell array was used to study dimensionality-related effects on osteogenic differentiation and plasticity of marrow stromal cells. To enable long-term culture of single cells in the array a novel surface functionalization technique was developed, using the principle of subtractive micro contact printing of fibronectin and surface passivation with a triblock-copolymer. Immunohistochemical stainings showed that when cultivated in 3D microenvironments, marrow stromal cells can be maintained in the wells for up to 7 days and be induced to commit to the osteogenic lineage. In conclusion, this work shows the modification of a 3D microwell array allowing the long term study of single stem cell plasticity and fate in controlled microenvironments.
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Acknowledgments
We are indebted to Prof. Marcus Textor and his group at ETH Zürich for sharing their knowledge on the microwell fabrication technique. The present study is supported by CCMX - MatLife (Competence Center for Materials Science and Technology – Materials for the Life Sciences), Switzerland.
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Markus Rottmar and Maria Håkanson have equally contributed to this work.
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Rottmar, M., Håkanson, M., Smith, M. et al. Stem cell plasticity, osteogenic differentiation and the third dimension. J Mater Sci: Mater Med 21, 999–1004 (2010). https://doi.org/10.1007/s10856-009-3926-7
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DOI: https://doi.org/10.1007/s10856-009-3926-7