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Palmitate Enhances Protein Tyrosine Phosphatase 1B (PTP1B) Gene Expression at Transcriptional Level in C2C12 Skeletal Muscle Cells

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Abstract

The factors responsible for regulating PTP1B expression in certain metabolic states have been unclear. We investigated the effects of palmitate on PTP1B gene transcription in C2C12 myotubes and myotubes co-cultured with J774A.1 macrophages. PTP1B mRNA and promoter activity levels were measured in C2C12 myotubes and direct or indirect co-culture of myotubes with macrophages using real time PCR and luciferase activity, respectively. Palmitate significantly induced PTP1B mRNA and promoter activity levels 1.36- and 1.32-fold, respectively, in C2C12 cells compared with the untreated myotubes. Palmitate showed a 2.3-fold increase in PTP1B mRNA level in myotubes co-cultured indirectly with macrophages. PTP1B promoter activity was also significantly induced by palmitate in direct (1.8-fold) and indirect (1.48-fold) co-culture of myotubes with macrophages. These data suggest that while palmitate treatment significantly induced PTP1B expression at the transcriptional level, the presence of an inflammatory process exerted by macrophages can augment this effect in the muscle cells.

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Acknowledgments

We greatly appreciate the assistance provided by the staff of the Biomedical Laboratory, Women Research Center, Alzahra University and the Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Iran

    Narges MohammadTaghvaei & Mohammad Taghikhani

  2. Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Reza Meshkani

  3. Endocrinology and Metabolism Research Centre, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Bagher Larijani

  4. Clinical Biochemistry, The Hospital for Sick Children, University of Toronto, Toronto, ON, Canada

    Khosrow Adeli

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  1. Narges MohammadTaghvaei
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  2. Reza Meshkani
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Correspondence to Mohammad Taghikhani.

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MohammadTaghvaei, N., Meshkani, R., Taghikhani, M. et al. Palmitate Enhances Protein Tyrosine Phosphatase 1B (PTP1B) Gene Expression at Transcriptional Level in C2C12 Skeletal Muscle Cells. Inflammation 34, 43–48 (2011). https://doi.org/10.1007/s10753-010-9206-3

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