Skip to main content

Advertisement

SpringerLink
Log in

Xanthii Fructus Inhibits Inflammatory Responses in LPS-Stimulated Mouse Peritoneal Macrophages

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Xanthii Fructus (XF) is an herb widely used in medicine for the treatment of a variety of inflammatory pathologies. In this study, using mouse peritoneal macrophages, we have examined whether XF affects nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-12p40 production induced by interferon (IFN)-γ and lipopolysaccharide (LPS). XF inhibits IFN-γ and LPS-induced NO production in a dose dependent manner. The decrease in NO synthesis was reflected as a decreased amount of inducible NO synthase protein. Furthermore, we also found that XF inhibits pro-inflammatory cytokine TNF-α production. However, treatment of XF in peritoneal macrophages had no effect on IL-12p40 production. These findings suggest that XF may be used in controlling macrophages-mediated inflammatory diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chu, T. R., and Y. C. Wei. 1965. Studies on the principal unsaturated fatty acids of the seed oil of Xanthium strumarium. Yao Hsueh Hsueh Pao 12:709–712.

    PubMed  Google Scholar 

  2. Kuo, Y. C., C. M. Sun, W. J. Tsai, J. C. Ou, W. P. Chen, and C. Y. Lin. 1998. Chinese herbs as modulators of human mesangial cell proliferation: Preliminary studies. J. Lab. Clin. Med. 132:76–85.

    Article  PubMed  Google Scholar 

  3. Hong, S. H., H. J. Jeong, and H. M. Kim. 2003. Inhibitory effects of Xanthii fructus extract on mast cell-mediated allergic reaction in murine model. J. Ethnopharmacol. 88:229–234.

    Article  PubMed  Google Scholar 

  4. Adams, D. O., and T. A. Hamilton. 1984. The cell biology of macrophage activation. Annu. Rev. Immunol. 2:283–318.

    Article  PubMed  Google Scholar 

  5. Schimmer, B. P., and K. L. Parker. 2001. Adrenocorticotropic hormone; adrenocortical steroids and their synthetic analogs; inhibitors of the synthesis and actions of adrenocortical hormones. In: The Pharmacological Basis of Therapeutics 10th edn., J. G. Hardman, L.E. Limbird, and A. Goodman Gilman, eds., McGraw-Hill, New York, pp. 1649–1677.

    Google Scholar 

  6. Nathan, C. F. 1992. Nitric oxide as a secretory product of mammalian cells. FASEB J. 6:3051.

    PubMed  Google Scholar 

  7. Xie, Q. W., H. J. J. Cho, R. A. Mumford, K. M. Swiderek, T. D. Lee, A. Ding, T. Troso, and C. Nathan. 1992. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science 256:225.

    PubMed  Google Scholar 

  8. Petros, A., D. Bennet, and P. Vallance. 1991. Effects of nitric oxide synthase inhibitor on hypotension in patients with septic shock. Lancet 338:1557–1558.

    Article  PubMed  Google Scholar 

  9. Thiemermann, C., and J. Vane. 1990. Inhibition of nitric oxide synthesis reduces the hypotension induced by bacterial lipopolysaccharides in the rat in vivo. Eur. J. Pharmacol. 182:591–595.

    Article  PubMed  Google Scholar 

  10. Evans, C. H. 1995. Nitric oxide: What role does it play in inflammation and tissue destruction? Agents Actions Supplement 47:107–116.

    Google Scholar 

  11. O’Shea, J. J., A. Ma, and P. Lipsky. 2002. Cytokines and autoimmunity. Nat. Rev. Immunol. 2:37–45.

    Article  PubMed  Google Scholar 

  12. Gorman, J. D., K. E. Sack, and J. C. Davis, Jr. 2002. Treatment of ankylosing spondylitis by inhibition of tumor necrosis factor alpha. New Engl. J. Med. 346:1349–1356.

    Article  PubMed  Google Scholar 

  13. Chung, H. S., H. J. Jeong, S. H. Hong, M. S. Kim, S. J. Kim, B. K. Song, I. S. Jeong, E. J. Lee, J. W. Ahn, S. H. Baek, and H. M. Kim. 2002. Induction of nitric oxide synthase by Oldenlandia diffusa in mouse peritoneal macrophages. Biol. Pharm. Bull. 25:1142–1146.

    Article  PubMed  Google Scholar 

  14. Jeong, H. J., H. S. Chung, H. J. An, J. B. Kim, E. M. Lee, E. J. Park, C. H. Jang, S. H. Hong, and H. M. Kim. 2003. Immune-enhancement effect of the herbal combination Allergina. Clin. Chim. Acta. 337:77–84.

    Article  PubMed  Google Scholar 

  15. Williams, D. H., and I. Fleming. 1995. Spectroscopic Methods in Organic Chemistry, Mcgraw-Hill Book Co., New York-London, pp. 63–169.

    Google Scholar 

  16. Hsu, H. Y., Y. P. Cheu, and M. Hong. 1982. The Chemical Constituents of Oriental Herbs. Oriental Healing Arts Institute, Los Angeles, California, pp. 1000–1005.

    Google Scholar 

  17. Koyanagi, M., K. Egashira, M. Kubo-Inoue, M. Usui, S. Kitamoto, H. Tomita, H. Shimokawa, and A. Takeshita. 2000. Role of transforming growth factor-beta1 in cardiovascular inflammatory changes induced by chronic inhibition of nitric oxide synthesis. Hypertension 35:86–90.

    PubMed  Google Scholar 

  18. Blackman, D. J., J. A. Morris-Thurgood, J. J. Atherton, G. R. Ellis, R. A. Anderson, J. R. Cockcroft, and M. P. Frenneaux. 2000. Endothelium-derived nitric oxide contributes to the regulation of venous tone in humans. Circulation 18:165–170.

    Google Scholar 

  19. Sharma, R., A. J. Coats, and S. D. Anker. 2000. The role of inflammatory mediators in chronic heart failure: Cytokines, nitric oxide, and endothelin-1. Int. J. Cardiol. 15:175–186.

    Article  Google Scholar 

  20. Fischer, L. G., D. J. Horstman, K. Hahnenkamp, N. E. Kechner, and G. F. Rich. 1999. Selective iNOS inhibition attenuates acetylcholine- and bradykinin-induced vasoconstriction in lipopolysaccharide-exposed rat lungs. Anesthesiology 91:1724–1732.

    Article  PubMed  Google Scholar 

  21. Kettelhut, I. C., W. Fiers, and A. L. Goldberg. 1987. The toxic effects of tumor necrosis factor in vivo and their prevention by cyclooxygenase inhibitors. P. Nat. Acad. Sci. USA. 84:4273–4277.

    Google Scholar 

  22. Connelly, L., M. Palacios-Callender, C. Ameixa, S. Moncada, and A. J. Hobbs. 2001. Biphasic regulation of NF-kappa B activity underlies the pro- and anti-inflammatory actions of nitric oxide. J. Immunol. 15:3873–3881.

    Google Scholar 

  23. Chung, H. S., H. J. An, H. J. Jeong, J. H. Won, S. H. Hong, and H. M. Kim. 2004. Water extract isolated from Chelidonium majus enhances nitric oxide and tumour necrosis factor-alpha production via nuclear factor-kappaB activation in mouse peritoneal macrophages. J. Pharm. Pharmacol. 56:129–134.

    Article  PubMed  Google Scholar 

  24. Remick, D. G., R. M. Strieter, M. K. Eskandari, D. T. Nguyen, M. A. Genord, C. L. Raiford, and S. L. Kunkel. 1990. Role of tumor necrosis factor-alpha in lipopolysaccharide-induced pathologic alterations. Am. J. Pathol. 136:49–60.

    PubMed  Google Scholar 

  25. Zhang, X., and D. C. Morrison. 1993. Pertussis toxin-sensitive factor differentially regulates lipopolysaccharide-induced tumor necrosis factor-alpha and nitric oxide production in mouse peritoneal macrophages. J. Immunol. 1:1011–1018.

    Google Scholar 

  26. Kaisho, T., O. Takeuchi, T. Kawai, K. Hoshino, and S. Akira. 2001. Endotoxin-induced maturation of MyD88-deficient dendritic cells. J. Immunol. 1:5688–5694.

    Google Scholar 

  27. Chae, Y. B., W. J. Kim, O. P. Zee, M. J. Ahn, and Y. J. No. 1998. Information of Korea Useful Herbal Medicine, Korea Research Institute of Chemical Technology, Taejeon, pp. 267–278.

    Google Scholar 

  28. Park, S. Y., E. Y. Moon, O. P. Zee, and E. K. Park. 1990. Immunostimulating activity of water extract from herbal drugs (I): Immunosuppressing activity of water extract from Xantium strumarium. Korea J. Immunology. 12:119.

    Google Scholar 

  29. Hsu, F. L., Y. C. Chem, and J. T. Cheng. 2000. Caffeic acid as active principle from the fruit of Xanthium strumarium to lower plasma glucose in diabetic rats. Planta Med. 66: 228–230.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea

    Hyo-Jin An, Hyun-Ja Jeong & Hyung-Min Kim

  2. College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, Republic of Korea

    Hyo-Jin An, Yun-Kyung Kim & Seung-Heon Hong

  3. Division of Beauty Arts, Jeonbuk Science College, Jeonegeup, Jeonbuk, 580-712, Republic of Korea

    Eun-Hee Lee

  4. Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea

    Woo-Jun Hwang

  5. Department of Emergency, Wonkwang University School of Medicine, Iksan, Jeonbuk, 570-749, Republic of Korea

    Su-Jin Yoo

  6. Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, 130-701, Republic of Korea

    Hyung-Min Kim

Authors
  1. Hyo-Jin An
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hyun-Ja Jeong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eun-Hee Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yun-Kyung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Woo-Jun Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Su-Jin Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Seung-Heon Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hyung-Min Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hyung-Min Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

An, HJ., Jeong, HJ., Lee, EH. et al. Xanthii Fructus Inhibits Inflammatory Responses in LPS-Stimulated Mouse Peritoneal Macrophages. Inflammation 28, 263–270 (2004). https://doi.org/10.1007/s10753-004-6049-9

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-004-6049-9

Key Words

Search

Navigation