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The role of dissolved organic matter bioavailability in promoting phytoplankton blooms in Florida Bay

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Abstract

The clear, shallow, oligotrophic waters of Florida Bay are characterized by low phytoplankton biomass, yet periodic cyanobacteria and diatom blooms do occur. We hypothesized that allochthonous dissolved organic matter (DOM) was providing a subsidy to the system in the form of bound nutrients. Water from four bay sites was incubated under natural light and dark conditions with enrichments of either DOM ( > 1 kD, 2×DOM) or inorganic nutrients (N+P). Samples were analyzed for bacterial numbers, bacterial production, phytoplankton biomass, phytoplankton community structure, and production, nutrients, and alkaline phosphatase (AP) activity. The influence of 2×DOM enrichment on phytoplankton biomass developed slowly during the incubations and was relatively small compared to nutrient additions. Inorganic nutrient additions resulted in an ephemeral bloom characterized initially as cyanobacterial and brown algae but which changed to dinoflagellate and/or brown algae by day six. The DIN:TP ratio decreased 10-fold in the N+P treatments as the system progressed towards N limitation. This ratio did not change significantly for 2×DOM treatments. In addition, these experiments indicated that both autotrophic and heterotrophic microbial populations in Florida Bay may fluctuate in their limitation by organic and inorganic nutrient availability. Both N+P and 2×DOM enrichments revealed significant and positive response in bioavailability of dissolved organic carbon (BDOC). Potential BDOC ranged from 1.1 to 35.5%, with the most labile forms occurring in Whipray Basin. BDOC at all sites was stimulated by the 2×DOM addition. Except for Duck Key, BDOC at all sites was also stimulated by the addition of N+P. BDOC was lower in the dry season than in the wet season (5.56% vs. 16.86%). This may be explained by the distinct chemical characteristics of the DOM produced at different times of year. Thus, both the heterotrophic and autotrophic microbial communities in Florida Bay are modulated by bioavailability of DOM. This has ramifications for the fate of DOM from the Everglades inputs, implicating DOM bioavailability as a contributing factor in regulating the onset, persistence, and composition of phytoplankton blooms.

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References

  • J. W. Ammerman F. Azam (1991) ArticleTitleBacterial 5′-nucleosidase activity in estuarine and coastal marine waters: characterization of enzyme activity Limnology and Oceanography 36 1427–1436 Occurrence Handle1:CAS:528:DyaK38Xhs12ktLc%3D

    CAS  Google Scholar 

  • R. M. W. Amon R. Benner (1996) ArticleTitleBacterial utilization of different size classes of dissolved organic matter Limnology and Oceanography 41 41–51 Occurrence Handle1:CAS:528:DyaK28XitlalsLk%3D

    CAS  Google Scholar 

  • G. B. Avery SuffixJr. J. D. Willey R. J. Kieber G. C. Shank R. F. Whitehead (2003) ArticleTitleFlux and bioavailability of Cape Fear River and rainwater dissolved organic carbon to Long Bay, southeastern United States Global Biogeochemical Cycles 17 1042 Occurrence Handle10.1029/2002GB001964 Occurrence Handle1:CAS:528:DC%2BD3sXmsFygtb0%3D

    Article  CAS  Google Scholar 

  • F. Azam T. Fenchel J. G. Field A. Meyer-reil F. Thingstad (1983) ArticleTitleThe ecological role of water-column microbes in the sea Marine Ecology Progress Series 10 257–263

    Google Scholar 

  • R. T. Bell (1993) Estimating production of heterotrophic bacterioplankton via incorporation of tritiated thymidine P. F. Kemp B. F. Sherr E. B. Sherr J. J. Cole (Eds) Handbook of Methods in Aquatic Microbial Ecology Lewis Publishers Boca Raton, Florida 495–503

    Google Scholar 

  • R. Benner E. R. Peele R. E. Hodson (1986) ArticleTitleMicrobial utilization of dissolved organic matter from leaves of the Red Mangrove, Rhizophora mangle, in the Fresh Creek Estuary, Bahamas Estuarine, Coastal and Shelf Science 23 607–619 Occurrence Handle1:CAS:528:DyaL2sXjvVSgsQ%3D%3D Occurrence Handle10.1016/0272-7714(86)90102-2

    Article  CAS  Google Scholar 

  • E. Bentzen W. D. Taylor E. S. Millard (1992) ArticleTitleThe importance of dissolved organic phosphorus to phosphorus uptake by limnetic plankton Limnology and Oceanography 37 217–231 Occurrence Handle1:CAS:528:DyaK38XlsFOitbo%3D

    CAS  Google Scholar 

  • Boyer, J. N., 2005. FY2004 Annual Report of the South Florida Coastal Water Quality Monitoring Network. SERC Tech. Rep. T-265. http://www.serc.fiu.edu/wqmnetwork/Report%20Archive/2004CWQMN.pdf.

  • J. N. Boyer J. W. Fourqurean R. D. Jones (1997) ArticleTitleSpatial characterization of water quality in Florida Bay and Whitewater Bay by multivariate analyses: zones of similar influence Estuaries 20 743–758 Occurrence Handle1:CAS:528:DyaK1cXjtVSmuw%3D%3D Occurrence Handle10.2307/1352248

    Article  CAS  Google Scholar 

  • J. N. Boyer J. W. Fourqurean R. D. Jones (1999) ArticleTitleSeasonal and long-term trends in water quality of Florida Bay (1989–1997) Estuaries 22 417–430 Occurrence Handle1:CAS:528:DyaK1MXmsVOmtr8%3D Occurrence Handle10.2307/1353208

    Article  CAS  Google Scholar 

  • J. N. Boyer R. D. Jones (1999) Effects of freshwater inputs and loading of phosphorus and nitrogen on the water quality of Eastern Florida Bay K. R. Reddy G. A. O’Connor C. L. Schelske (Eds) Phosphorus Biogeochemistry in Sub-tropical Ecosystems CRC/Lewis Publishers Boca Raton, FL 545–561

    Google Scholar 

  • Boynton W. R., W. M. Kemp, C. W. Keefe, 1982. A comparative analysis of nutrients and other factors influencing estuarine phytoplankton production. In Kennedy, V. (ed.), Estuarine Comparisons. Academic Press, 69–90.

  • Brand, L., 1999. Nutrient bioassays and the Redfield ratio in Florida Bay. Abstracts of the 1999 Florida Bay and Adjacent Marine Systems Science Conference. Key Largo, FL, 78–79.

  • J. B. C. Bugden M. A. Guerrero R. D. Jones (1998) ArticleTitleSpatial and temporal variation of marine bacterioplankton in Florida Bay, USA Journal of Coastal Research 14 1304–1313

    Google Scholar 

  • P. Carlsson E. Graneli (1993) ArticleTitleAvailability of humic bound nitrogen for coastal phytoplankton Estuarine, Coastal and Shelf Science 36 433–447 Occurrence Handle1:CAS:528:DyaK3sXmtV2qsL4%3D Occurrence Handle10.1006/ecss.1993.1026

    Article  CAS  Google Scholar 

  • D. A. Caron (1994) ArticleTitleInorganic nutrients, bacteria, and the microbial loop Microbial Ecology 28 295–298 Occurrence Handle1:CAS:528:DyaK2MXit1Wktbk%3D Occurrence Handle10.1007/BF00166820

    Article  CAS  Google Scholar 

  • A. D. Cembella N. J. Antia P. J. Harrison (1984) ArticleTitleThe utilization of inorganic and organic phosphorus compounds as nutrients by eukaryotic microalgae: a multidisciplinary perspective. Part 1 CRC Critical Reviews in Microbiology 10 317–391 Occurrence Handle6321101 Occurrence Handle1:CAS:528:DyaL2cXhtlGntb4%3D

    PubMed  CAS  Google Scholar 

  • Chrost, R. J., 1990. Microbial ectoenzymes in aquatic environments. In Overbeck, J. & R. J. Chrost (eds), Aquatic Microbial Ecology: Biochemical and Molecular Approaches. Springer-Verlag, 47–78.

  • A. W. Coleman (1980) ArticleTitleEnhanced detection of bacteria in natural environments by flourochrome staining of DNA Limnology and Oceanography 25 948–951

    Google Scholar 

  • J. B. Cotner R. H. Sada H. Bootsma T. Johengen J. F. Cavaletto W. S. Gardner (2000) ArticleTitleNutrient limitation of heterotrophic bacteria in Florida Bay Estuaries 23 611–620 Occurrence Handle1:CAS:528:DC%2BD3MXkvV2ntQ%3D%3D Occurrence Handle10.2307/1352888

    Article  CAS  Google Scholar 

  • P. A. Giorgio Particledel J. J. Cole (1998) ArticleTitleBacterioplankton growth efficiency in aquatic systems Annual Review of Ecological Systematics 29 503–541 Occurrence Handle10.1146/annurev.ecolsys.29.1.503

    Article  Google Scholar 

  • del Giorgio, P. A. & J. Davis, 2003. Patterns in dissolved organic matter lability and consumption across aquatic ecosystems. In Findlay, S. E. G. & R. L. Sinsabaugh (eds), Aquatic Ecosystems: Interactivity of Dissolved Organic Matter. Academic Press, 399–424.

  • H. W. Ducklow C. A. Carlson (1992) ArticleTitleOceanic bacterial production Advances in Microbial Ecology 12 113–181

    Google Scholar 

  • J. W. Fourqurean R. D. Jones J. C. Zieman (1993) ArticleTitleProcesses influencing water column nutrient characteristics and phosphorus limitation of phytoplankton biomass in Florida Bay, FL, USA: inferences from spatial distributions Estuarine, Coastal and Shelf Science 36 295–314 Occurrence Handle1:CAS:528:DyaK3sXlsVKjsb0%3D Occurrence Handle10.1006/ecss.1993.1018

    Article  CAS  Google Scholar 

  • T. A. Frankovich R. D. Jones (1998) ArticleTitleA rapid, precise, and sensitive method for the determination of total nitrogen in natural waters Marine Chemistry 60 227–234 Occurrence Handle1:CAS:528:DyaK1cXivVWgsLs%3D Occurrence Handle10.1016/S0304-4203(97)00100-X

    Article  CAS  Google Scholar 

  • H. Garaudet J. L. Berthon B. Buisson (1997) ArticleTitleComparison of the daily alkaline phosphatase activity of a cyanobacterium (Microcystis aeruginosa) and a diatom (Synedra capitata) Comptes rendus de l’Académie des sciences. Série III, Sciences de la vie 320 451–458 Occurrence Handle10.1016/S0764-4469(97)81972-5

    Article  Google Scholar 

  • P. M. Glibert C. A. Heil D. Hollander M. Revilla A. Hoare J. Alexander S. Murasko (2004) ArticleTitleEvidence for dissolved organic nitrogen and phosphorus uptake during a cyanobacterial bloom in Florida Bay Marine Ecology Progress Series 280 73–83

    Google Scholar 

  • S. Hashimoto K. Fujiwara K. Fuwa (1985) ArticleTitleRelationship between alkaline phosphatase activity and orthophosphate in the present Tokyo Bay Journal of Environmental Science and Health, Part A 20A 781–908 Occurrence Handle10.1080/10934528509375258

    Article  Google Scholar 

  • J. G. Holmquist G. V. N. Powell S. M. Sogard (1989) ArticleTitleSediment, water level and water temperature characteristics of Florida Bay’s grass-covered mud banks Bulletin of Marine Science 44 348–364

    Google Scholar 

  • R. Jaffe J. N. Boyer X. Lu N. Maie C. Yang N. Scully (2003) ArticleTitleSource characterization of dissolved organic matter in a subtropical mangrove-dominated estuary by fluorescence analysis Marine Chemistry 84 195–210 Occurrence Handle10.1016/j.marchem.2003.08.001 Occurrence Handle1:CAS:528:DC%2BD3sXpsFeku78%3D

    Article  CAS  Google Scholar 

  • Y. Kitano M. Okumura M. Idogaki (1978) ArticleTitleUptake of phosphate ions by calcium carbonate Geochemistry Journal 12 29–37 Occurrence Handle1:CAS:528:DyaE1cXmtVeku7s%3D

    CAS  Google Scholar 

  • P. J. Lavrentyev H. A. Bootsma T. H. Johengen J. F. Cavaletto W. S. Gardner (1998) ArticleTitleMicrobial plankton response to resource limitation: insights from the community structure and seston stoichiometry in Florida Bay, USA Marine Ecology Progress Series 165 45–57 Occurrence Handle1:CAS:528:DyaK1cXjtlGqtL8%3D

    CAS  Google Scholar 

  • Lewitus, A. J., E. T. Koepfler & R. J. Pigg, 2000. Use of dissolved organic nitrogen by a salt marsh phytoplankton bloom community. Limnology and Lake Management 2000+. Proceedings of the Kinneret Symposium, Ginnosar, Israel, Sept. 1998. No. 55, pp. 15–29. Advances in limnology. Stuttgart.

  • N. Maie J. N. Boyer C. Yang R. Jaffé (2006) ArticleTitleSpatial, geomorphological, and seasonal variability of CDOM in estuaries of the Florida Coastal Everglades Hydrobiologia 569 135–150 Occurrence Handle1:CAS:528:DC%2BD28XnsFyjur4%3D

    CAS  Google Scholar 

  • M. Nausch G. Nausch (2004) ArticleTitleBacterial utilization of phosphorus pools after nitrogen and carbon amendment and its relation to alkaline phosphatase activity Aquatic Microbial Ecology 37 237–245

    Google Scholar 

  • E. J. Phlips S. Badylak (1996) ArticleTitleSpatial variability in phytoplankton standing crop and composition in a shallow inner-shelf lagoon, Florida Bay, Florida Bulletin of Marine Science 58 203–216

    Google Scholar 

  • E. J. Phlips S. Badylak T. C. Lynch (1999) ArticleTitleBlooms of the picoplanktonic cyanobacterium Synechococcus in Florida Bay, a subtropical inner-shelf lagoon Limnology and Oceanography 44 1166–1175

    Google Scholar 

  • E. J. Phlips C. Zeman P. Hansen (1989) ArticleTitleGrowth, photosynthesis, nitrogen fixation, and carbohydrate production by a unicellular cyanobacterium, Synechococcus sp. (Cyanophyta) Journal of Applied Phycology 1 137–145

    Google Scholar 

  • K. G. Porter Y. Feig (1980) ArticleTitleThe use of DAPI for identifying and counting aquatic microflora Limnology and Oceanography 25 934

    Google Scholar 

  • D. Rudnick Z. Chen D. Childers T. Fontaine J. N. Boyer (1999) ArticleTitlePhosphorus and nitrogen inputs to Florida Bay: the importance of the Everglades watershed Estuaries 22 398–416 Occurrence Handle1:CAS:528:DyaK1MXmsVOmtr4%3D Occurrence Handle10.2307/1353207

    Article  CAS  Google Scholar 

  • N. M. Sculley N. Maie S. K. Dailey J. N. Boyer R. Jaffe (2004) ArticleTitlePhotochemical and microbial transformation of plant derived dissolved organic matter in the Florida Everglades Limnology and Oceanography 49 1667–1678 Occurrence Handle10.4319/lo.2004.49.5.1667

    Article  Google Scholar 

  • R. E. H. Smith J. Kalff (1981) ArticleTitleThe effect of phosphorus limitation on algal growth rates: evidence from alkaline phosphatase Canadian Journal of Fisheries and Aquatic Sciences 38 1421–1427 Occurrence Handle1:CAS:528:DyaL38Xot1CgtQ%3D%3D

    CAS  Google Scholar 

  • W. V. Sobszak J. E. Cloern A. D. Jassby A. B. Muller-Solger (2002) ArticleTitleBioavailability of organic matter in a highly disturbed estuary: the role of algal and detrital resources Proceedings of the National Academy of Science 99 8101–8105 Occurrence Handle10.1073/pnas.122614399

    Article  Google Scholar 

  • L. Solorzano J. H. Sharp (1980) ArticleTitleDetermination of total dissolved phosphorus and particulate phosphorus in natural waters Limnology and Oceanography 25 754–758 Occurrence Handle1:CAS:528:DyaL3MXnsFSg Occurrence Handle10.4319/lo.1980.25.4.0754

    Article  CAS  Google Scholar 

  • Steidinger, K. A., & E. J. Phlips, 1996. Florida Bay microalgal blooms: composition, abundance, and distribution. Abstracts of the 1996 Florida Bay Science Conference. Florida Sea Grant, 77–79.

  • Stepanauskas, R., 2000. Utilization of terrestrially derived dissolved organic nitrogen. Dissertation. Lund University, Lund, Sweden.

  • Y. Tezuka (1990) ArticleTitleBacterial regeneration of ammonium and phosphate as affected by the carbon:nitrogen:phosphorus ratio of organic substrates Microbial Ecology 19 227–238 Occurrence Handle1:CAS:528:DyaK3cXlvVOktbg%3D Occurrence Handle10.1007/BF02017167

    Article  CAS  Google Scholar 

  • Turney, W. J. & B. F. Perkins, 1972. Molluscan distribution in Florida Bay. Sedimenta III. RSMAS, University of Miami. 37 pp.

  • N. A. D. Waser P. J. Harrison B. Nielsen S. E. Calvert D. H. Turpin (1998) ArticleTitleNitrogen isotope fractionation during the uptake and assimilation of nitrate, nitrite, ammonium, and urea by a marine diatom Limnology and Oceanography 43 215–224 Occurrence Handle1:CAS:528:DyaK1cXjvFWju78%3D Occurrence Handle10.4319/lo.1998.43.2.0215

    Article  CAS  Google Scholar 

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Boyer, J.N., Dailey, S.K., Gibson, P.J. et al. The role of dissolved organic matter bioavailability in promoting phytoplankton blooms in Florida Bay. Hydrobiologia 569, 71–85 (2006). https://doi.org/10.1007/s10750-006-0123-2

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