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Increased expression of Gem after rat sciatic nerve injury

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Abstract

Gem belongs to the Rad/Gem/Kir subfamily of Ras-related GTPases, whose expression is induced in several cell types upon activation by extracellular stimuli. Two functions of Gem have been demonstrated, including regulation of voltage-gated calcium channel activity and inhibition of Rho kinase-mediated cytoskeletal reorganization, such as stress fiber formation and neurite retraction. Because of the essential relationship between actin reorganization and peripheral nerve regeneration, we investigated the spatiotemporal expression of Gem in a rat sciatic nerve crush (SNC) model. After never injury, we observed that Gem had a significant up-regulation from 1 day, peaked at day 5 and then gradually decreased to the normal level. At its peak expression, Gem expressed mainly in Schwann cells (SCs) and macrophages of the distal sciatic nerve segment, but had few colocalization in axons. In addition, the peak expression of Gem was in parallel with PCNA, and numerous SCs expressing Gem were PCNA positive. Thus, all of our findings suggested that Gem may be involved in the pathophysiology of sciatic nerve after SNC.

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Abbreviations

SNC:

Sciatic nerve crush

SCs:

Schwann cells

ROK:

Rho kinase

CK2:

Casein kinase 2

ERK:

Extracellular signal-regulated kinase

PAGE:

Polyacrylamide gel electrophoresis

ECL:

Enhanced chemiluminescence system

BSA:

Bovine serum albumin

DAB:

Diaminobenzidin

PCNA:

Proliferating cell nuclear antigen

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No.81171140, No.21077061 and No. 31071288); Natural Science Foundation of Jiangsu province (No.BK2009161); A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, People’s Republic of China

    Youhua Wang, Xinghai Cheng, Zhengming Zhou, Hao Wu, Long Long & Guangfei Xu

  2. The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, People’s Republic of China

    Xingxing Gu

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  1. Youhua Wang
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  2. Xinghai Cheng
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  3. Zhengming Zhou
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  4. Hao Wu
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  5. Long Long
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  6. Xingxing Gu
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  7. Guangfei Xu
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Correspondence to Xingxing Gu or Guangfei Xu.

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Youhua Wang and Xinghai Cheng are contributed equally to this work.

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Wang, Y., Cheng, X., Zhou, Z. et al. Increased expression of Gem after rat sciatic nerve injury. J Mol Hist 44, 27–36 (2013). https://doi.org/10.1007/s10735-012-9459-2

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  • DOI: https://doi.org/10.1007/s10735-012-9459-2

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