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Accumulation of CD1a-positive Langerhans cells and mast cells in actinic cheilitis

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Abstract

LCs and MCs are known to be directly influenced by UV radiation. This study investigated the presence of Langerhans cells (LCs) and mast cells (MCs) in actinic cheilitis (AC) exhibiting epithelial dysplasia (ED). Using immunohistochemistry for CD1a and mast cell tryptase, LCs and MCs density was assessed in 35 cases of AC with different degrees of ED. LCs were found in 32 cases of AC whereas MCs were found in all cases. There was an increase in LCs density irrespective of degree of ED when the cases were compared to normal lip mucosa (P = 0.04343). No statistical difference in LCs density was observed regarding the different degrees of dysplasia (P > 0.05). Significant difference in MCs density between mild and moderate dysplasia and normal lip mucosa was found (P < 0.05). No significant correlation between LCs and MCs was seen (P = 0.1258). Although no correlation could be established between LCs and MCs and the different degrees of ED; it is possible that the accumulation of LCs plays an immunostimulatory and protective role in the defense against progression of dysplasia. Further studies are necessary to determine the role of MCs in the development of AC.

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References

  • Aaltomaa S, Lipponen P, Papinaho S et al (1993) Mast cells in breast cancer. Anticancer Res 13:785–788

    PubMed  Google Scholar 

  • Albuquerque RL Jr, Miguel MC, Costa AL et al (2003) Correlation of c-erbB-2 and S-100 expression with the malignancy grading and anatomical site in oral squamous cell carcinoma. Int J Exp Pathol 84:259–265

    Article  PubMed  Google Scholar 

  • Antonopoulos C, Cumberbatch M, Mee JB et al (2008) IL-18 is a key proximal mediator of contact hypersensitivity and allergen-induced Langerhans cell migration in murine epidermis. J Leukocyte Biol 83:1–7

    Google Scholar 

  • Bosset S, Bonnet-Duquennoy M, Barre P et al (2003) Photoageing shows histological features of chronic skin inflammation without clinical and molecular abnormalities. Br J Dermatol 149:826–835

    Article  PubMed  Google Scholar 

  • Cataldo E, Doku HC (1981) Solar cheilitis. J Dermatol Surg Oncol 7:985–989

    Google Scholar 

  • Caughey GH (2007) Mast cell tryptases and chymases in inflammation and host defense. Immunol Rev 217:141–154

    Article  PubMed  Google Scholar 

  • Cavalcante ASR, Anbinder AL, Carvalho YR (2008) Actinic Cheilitis: clinical and histological features. J Oral Maxillofac Surg 66:498–503

    Article  PubMed  Google Scholar 

  • Chan Jk, Magistris A, Loizzi V et al (2005) Mast cell density, angiogenesis, blood clotting, and prognosis in women with advanced ovarian cancer. Ginecol Oncol 99:20–25

    Article  Google Scholar 

  • Chen CC, Grimbaldeston MA, Tsai M (2005) Identification of mast cell progenitors in adult mice. Proc Natl Acad Acad Sci USA 102:11408–11413

    Article  Google Scholar 

  • Clydesdale GJ, Dandie GW, Muller HK (2001) Ultraviolet light induce injury: Immunological and inflammatory effects. Immunol Cell Biol 79:547–568

    Article  PubMed  Google Scholar 

  • Costa NL, Oton-Leite AF, Cheim-Júnior AP et al (2009) Density and migration of mast cells in lip squamous cell carcinoma and actinic cheilitis. Histol Histopathol 24:457–465

    PubMed  Google Scholar 

  • Cumberbatch M, Dearman RJ, Groves RW (2002) Differential regulation of epidermal Langerhans cell migration by interleukins (IL)-1alpha and IL-1beta during irritant- and allergen-induced cutaneous immune responses. Toxicol Appl Pharmacol 182:126–135

    Article  PubMed  Google Scholar 

  • Cutler CW, Jotwani R (2004) Antigen-presentation and the role of dendritic cells in periodontitis. Periodontol 35:135–157

    Google Scholar 

  • Cutler CW, Jotwani R (2006) Dendritic cells at the oral mucosal interface. J Dent Res 85:678–689

    Article  PubMed  Google Scholar 

  • De Melo MR Jr, Araújo-Filho JL, Patu VJ (2006) Langerhans cells in cutaneous tumours: immunohistochemistry study using a computer image analysis system. J Mol Hist 37:321–325

    Article  Google Scholar 

  • Endoh I, DI Girolamo N, Hampartzoumian T (2007) Ultraviolet B irradiation selectively increases the production of interleukin-8 in human cord blood-derived mast cells. Exp Immunol 148:161–167

    Article  Google Scholar 

  • Erbagci Z, Erkiliç S (2002) Can smoking and/or occupational UV exposure have any role in the development of the morpheaform basal cell carcinoma? A critical role for peritumoral mast cells. Int J Dermatol 41:275–278

    Article  PubMed  Google Scholar 

  • Facy V, Flouret V, Régnier M et al (2005) Reactivity of Langerhans cells in human reconstructed epidermis to known allergens and UV radiation. Toxicol Vitro 19:787–795

    Article  Google Scholar 

  • Fisher ER, Paik RM, Rockette H et al (1989) Prognostic significance of eosinophils and mast cell in rectal cancer. Human Pathol 20:159–163

    Article  Google Scholar 

  • Fleischmann A, Schlomm T, Kollermann J et al (2009) Immunological microenvironment in prostate cancer: high mast cell densities are associated with favorable tumor characteristics and good prognosis. Prostate 69:976–981

    Article  PubMed  Google Scholar 

  • Fukushima H, Ohsawa M, Ikura I et al (2006) Mast cell in difuse large B-cell lymphoma; their role in fibrosis. Histopathology 49:498–505

    Article  PubMed  Google Scholar 

  • Gale N, Pich BZ, Sidransky D et al (2005) Epithelial precursor lesions. In: Barnes L, Eveson JW, Reichart P, Sidransky D (eds) World Heatlth Organisation classification of tumours—pathology & genetics—head and neck tumours. IARC press scientific publications, Lyon, pp 177–179

    Google Scholar 

  • Gomes AP, Johann JE, Lovatto GG et al (2008) Comparative analysis of the mast cell density in normal oral mucosa, actinic cheilitis and lip squamous cell carcinoma. Braz Dent J 19:186–189

    Google Scholar 

  • Grimbaldeston MA, Skov L, Finlay-Jones JJ et al (2002) Increased dermal mast cell prevallence and susceptibility to development of basal carcinoma in humans. Methods 28:90–96

    Article  PubMed  Google Scholar 

  • Grimbaldeston MA, Finlay-Jones JJ, Hart PH (2006) Mast cells in photodamaged skin: what is their role in skin cancer? Photochem Photobiol Sci 5:177–183

    Article  PubMed  Google Scholar 

  • Guhl S, Stefaniak R, Strathmann M et al (2005) Bivalent effect of UV light on human skin mast cells-low-level mediator release at baseline but potent suppression upon mast cell triggering. J Invest Dermatol 124:453–456

    Article  PubMed  Google Scholar 

  • Hahn WC, Weinberg RA (2002) Rules for making human tumor cells. N Engl J Med 347:1593–1603

    Google Scholar 

  • Hart PH, Grimbaldeston MA, Swift GJ et al (1998) TNF modulates susceptibility to UVB-induced systemic immunomodulation in mice by effects on dermal mast cell prevalence. Eur J Immunol 28:2893–2901

    Article  PubMed  Google Scholar 

  • Hart PH, Grimbaldeston MA, Finlay-Jones JJ (2000) Mast cells in UV-B-induced immunosuppression. J Photochem Photobiol Biol 55:81–87

    Article  Google Scholar 

  • Hart PH, Grimbaldeston MA, Finlay-Jones JJ (2001) Sunlight, immunosuppression and skin cancer: role of histamine and mast cells. Clin Exp Pharmacol Physiol 28:1–8

    Article  PubMed  Google Scholar 

  • Henz B (2008) Exploring the mast cell enigma: a personal reflection of what remains to be done. Exp Dermatol 17:91–99

    Article  PubMed  Google Scholar 

  • Holiková Z, Hercogavá J, Pizák J et al (2001) Dendritic cells in the skin and mucosa: what′s new. J Eur Acad Dermatol Venerol 15:116–120

    Article  Google Scholar 

  • Hubert P, Bousarghin L, Greimers R et al (2005) Production of large numbers of Langerhans cells with intraephithelial migration ability in vitro. Exp Dermatol 14:469–477

    Article  PubMed  Google Scholar 

  • Humphreys TR, Monteiro MR, Murphy GF (2000) Mast cells and dendritic cells in basal cell carcinoma stroma. Dermatol Surg 26:200–204

    Article  PubMed  Google Scholar 

  • Huttunen M, Harvina IT (2005) Mast cell tryptase and chymase in chronic leg ulcers: chymase is potentially destructive to epithelium and is controlled by proteinase inhibitors. Br J Dermatol 152:1149–1160

    Article  PubMed  Google Scholar 

  • Iamaroon A, Pongsiriwet S, Jittidecharaks S et al (2003) Increase of mast cells and tumor angiogenesis in oral squamous cell carcinoma. J Oral Pathol Med 35:195–199

    Article  Google Scholar 

  • Kankkunen JP, Harvina IT, Naukkarinen A (1997) Quantitative analysis of triptase and chymase containing mast cells in begin and malignant breast lesions. Int J Cancer 72:385–388

    Article  PubMed  Google Scholar 

  • Kaugars GE, Pillion T, Svirsky JA et al (1999) Actinic cheilitis: a review of 152 cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 88:181–186

    Article  PubMed  Google Scholar 

  • Kolgen W, Both H, Van Weelden H et al (2002) Epidermal Langerhans cell depletion after artificial ultraviolet B irradiation of human skin In vivo: apoptosis versus migration. J Invest Dermatol 118:812–817

    Article  PubMed  Google Scholar 

  • La Rocca G, Anzalone R, Corrao S et al (2000) Cd1a down-regulation in primary invasive ductal breast carcinoma may predict regional lymph node invasion and patient outcome. Histopathology 52:203–212

    Article  Google Scholar 

  • Le Bitoux MA, Stamenkovic I (2008) Tumor-host interactions: the role of inflammation. Histochem Cell Biol 130:1079–1090

    Article  PubMed  Google Scholar 

  • Lin WW, Karin M (2007) A cytokine-mediated link between innate immunity, inflammation, and cancer. J Clin Invest 117:1175–1183

    Article  PubMed  Google Scholar 

  • Markopoulos A, Albanidou-Farmaki E, Kayavis I (2004) Actinic cheilitis: clinical and pathologic characteristics in 65 cases. Oral Dis 10:212–216

    Article  PubMed  Google Scholar 

  • Maurer M, Theoharides T, Granstein RD et al (2003) What is the physiological function of mast cell? Exp Dermatol 12:886–910

    Article  PubMed  Google Scholar 

  • Meunier L, Bata-Csorgo Z, Cooper KD (1995) In human dermis, ultraviolety radiation induces expansion of a CD36 + CD11b + CD1-macrophage subset by infiltration and proliferation; CD1 + Langerhans-like dendritic antigen presenting cells are concomitantly depleted. J Invest Dermatol 105:782–788

    Article  PubMed  Google Scholar 

  • Michailidou EZ, Markopoulos AK, Antoniades DZ (2008) Mast cell and angiogenesis in oral malignant and premalignant lesions. Open Dent J 28:126–132

    Google Scholar 

  • Miyagi J, Kinjo T, Tsuhako K et al (2001) Extremely high Langerhans cell infiltration contributes to the favourable prognosis of HPV-infected squamous cell carcinoma and adenocarcinoma of the lung. Histopathology 38:355–367

    Article  PubMed  Google Scholar 

  • Mowat AM (2005) Dendritic cells and immune responses to orally administered antigens. Vaccine 23:1797–1799

    Article  PubMed  Google Scholar 

  • Nagata M, Shijubo N, Walls AF (2003) Chymase-positive mast cells in small sized adenocarcinoma of the lung. Virchows Arch 443:565–573

    Article  PubMed  Google Scholar 

  • Norval M, Mcloone P, Lesiak A (2008) The effect of chronic ultraviolet radiation on the human immune system. Photochem Photobiol 84:19–28

    Article  PubMed  Google Scholar 

  • Picascia DD, Robinson JK (1987) Actinic cheilitis: a review of the etiology, differential diagnosis and treatment. J Am Acad Dermatol 17:255–264

    Article  PubMed  Google Scholar 

  • Rattis FM, Concha M, Dalbiez-Gauthier C et al (1998) Effects of ultraviolet B radiation on human Langerhans cells. Functional alteration of CD86 upregulation and induction of apoptotic cell death. J Invest Dermatol 111:373–379

    Article  PubMed  Google Scholar 

  • Ribatti D, Finato N, Crivellato E et al (2007) Angiogenesis and mast cells in human breast cancer sentinel lymph nodes with and without micrometastases. Histopathology 51:837–842

    Article  PubMed  Google Scholar 

  • Rodewald HR, Dessing M, Dvorak AM (1996) Identification of a committed precursor for the mast cell lineage. Science 271:818–822

    Article  PubMed  Google Scholar 

  • Rojas IG, Martinez A, Pineda A (2004) Increased mast cell density and protease content in actinic cheilitis. J Oral Pathol Med 33:567–573

    Article  PubMed  Google Scholar 

  • Rojas IG, Spencer ML, Martinez A (2005) Characterization of mast cell subpopulations in lip cancer. J Oral Pathol Med 34:268–273

    Article  PubMed  Google Scholar 

  • Ruoss SJ, Hartmann T, Caughey GH (1991) Mast cell tryptase is a mitogen for cultured fibroblasts. J Clin Invest 88:493–499

    Article  PubMed  Google Scholar 

  • Santos JN (2000) Expressão imuno-histoquímica de citoqueratinas, CD1a, S-100, p53, p21, PCNA E Ki-67 em queilite actínica. Thesis, University of São Paulo

  • Santos JN, Souza SO, Nunes FD et al (2003) Altered cytokeratin expression in actinic cheilitis. J Cutan Pathol 30:237–241

    Article  PubMed  Google Scholar 

  • Schroder JM, Reich K, Kabashima K et al (2006) Who is really in control of skin immunity under physiological circumstances—lymphocytes, dendritic cells or keratinocytes? Exp Dermatol 15:913–929

    PubMed  Google Scholar 

  • Schwartz A, Grabbe S, Grosse-Heitemeyr K et al (1998) Ultraviolet light-induced immunetolerance is mediated via the Fas/Fas-ligand system. J Immunol 160:4262–4270

    Google Scholar 

  • Takashima A (2005) UVB-dependent modulation of epidermal cytokine network roles in UVB-induced depletion of Langerhans cells and dendritic epidermal T cells. J Dermatol 22:876–887

    Google Scholar 

  • Timares L, Katyar SK, Elmets CA (2008) DNA damage, apoptosis and Langerhans cells–activators of UV-induced immune tolerance. Photochem Photobiol 84:422–436

    Article  PubMed  Google Scholar 

  • Valent P, Schernthaner GH, Sperr WR et al (2001) Variable expression of activation-linked surface antigens on human mast cells in health and disease. Immunol Rev 179:74–81

    Article  PubMed  Google Scholar 

  • Wang HW, Tedla N, Hunt JE et al (2005) Mast cell accumulation and cytokine expression in the tight skin mouse model of scleroderma. Exp Dermatol 14:292–295

    Article  Google Scholar 

  • Welsh TJ, Green RH, Richardson D et al (2005) Macrophage and mast-cell invasion of tumor ell isçets confers a marked survival advantage in non-small-cell lung cancer. J Clin Oncol 23:8959–8967

    Article  PubMed  Google Scholar 

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Acknowledgments

This study was supported by CNPq (Edital Universal, processo 485964/2006-6).

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Authors and Affiliations

  1. Department of Oral Pathology, Laboratory of Oral Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil

    Caliandra Pinto Araújo

  2. Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil

    Clarissa Araújo Silva Gurgel, Eduardo Antônio Gonçalves Ramos & Aryon de Almeida Barbosa Júnior

  3. Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil

    Valéria Souza Freitas

  4. Department of Stomatology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil

    Luciana Maria Pedreira Ramalho

  5. Department of Oral Pathology, Laboratory of Oral Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil

    Jean Nunes dos Santos

  6. Faculdade de Odontologia, Universidade Federal da Bahia, Avenida Araújo Pinho, 62, Canela, Salvador, Bahia, 40110-150, Brazil

    Jean Nunes dos Santos

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  1. Caliandra Pinto Araújo
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  2. Clarissa Araújo Silva Gurgel
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  3. Eduardo Antônio Gonçalves Ramos
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  4. Valéria Souza Freitas
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  7. Jean Nunes dos Santos
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Correspondence to Jean Nunes dos Santos.

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Araújo, C.P., Gurgel, C.A.S., Ramos, E.A.G. et al. Accumulation of CD1a-positive Langerhans cells and mast cells in actinic cheilitis. J Mol Hist 41, 357–365 (2010). https://doi.org/10.1007/s10735-010-9297-z

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  • DOI: https://doi.org/10.1007/s10735-010-9297-z

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