Abstract
In the adrenal medulla, binding of the immediate early gene (IEG) proteins, EGR-1 (ZIF-268/KROX-24/NGFI-A) and AP-1, to the tyrosine hydroxylase (Th) proximal promoter mediate inducible Th expression. The current study investigated the potential role of EGR-1 in inducible Th expression in the olfactory bulb (OB) since IEGs bound to the AP-1 site in the Th proximal promoter are also necessary for activity-dependent OB TH expression. Immunohistochemical analysis of a naris-occluded mouse model of odor deprivation revealed weak EGR-1 expression levels in the OB glomerular layer that were activity-dependent. Immunofluorescence analysis indicated that a majority of glomerular cells expressing EGR-1 also co-expressed TH, but only small subset of TH-expressing cells contained EGR-1. By contrast, granule cells, which lack TH, exhibited EGR-1 expression levels that were unchanged by naris closure. Together, these finding suggest that EGR-1 mediates activity-dependent TH expression in a subset of OB dopaminergic neurons, and that there is differential regulation of EGR-1 in periglomerular and granule cells.
This is a preview of subscription content, log in via an institution to check access.
References
Alonso M, Viollet C, Gabellec MM, Meas-Yedid V, Olivo-Marin JC, Lledo PM (2006) Olfactory discrimination learning increases the survival of adult-born neurons in the olfactory bulb. J Neurosci 26:10508–10513. doi:10.1523/JNEUROSCI.2633-06.2006
Baker H, Kawano T, Margolis FL, Joh TH (1983) Transneuronal regulation of tyrosine hydroxylase expression in olfactory bulb of mouse and rat. J Neurosci 3:69–78
Baker H, Morel K, Stone DM, Maruniak JA (1993) Adult naris closure profoundly reduces tyrosine hydroxylase expression in mouse olfactory bulb. Brain Res 614:109–116. doi:10.1016/0006-8993(93)91023-L
Baker H, Liu N, Chun HS, Saino S, Berlin R, Volpe B, Son JH (2001) Phenotypic differentiation during migration of dopaminergic progenitor cells to the olfactory bulb. J Neurosci 21:8505–8513
Cave JW, Baker H (2008) Dopamine systems in the forebrain. In: Pasterkamp RJ, Smidt MP, Burbach JPH (eds) Development and engineering of dopamine neurons. Landes BioScience, Austin
Inaki K, Takahashi YK, Nagayama S, Mori K (2002) Molecular-feature domains with posterodorsal-anteroventral polarity in the symmetrical sensory maps of the mouse olfactory bulb: mapping of odourant-induced Zif268 expression. Eur J NeuroSci 15:1563–1574. doi:10.1046/j.1460-9568.2002.01991.x
Inoue T, Ota M, Ogawa M, Mikoshiba K, Aruga J (2007) Zic1 and Zic3 regulate medial forebrain development through expansion of neuronal progenitors. J Neurosci 27:5461–5473. doi:10.1523/JNEUROSCI.4046-06.2007
Johnson BA, Woo CC, Duong H, Nguyen V, Leon M (1995) A learned odor evokes an enhanced Fos-like glomerular response in the olfactory bulb of young rats. Brain Res 699:192–200. doi:10.1016/0006-8993(95)00896-X
Kohwi M, Osumi N, Rubenstein JL, Alvarez-Buylla A (2005) Pax6 is required for making specific subpopulations of granule and periglomerular neurons in the olfactory bulb. J Neurosci 25:6997–7003. doi:10.1523/JNEUROSCI.1435-05.2005
Liu N, Cigola E, Tinti C, Jin BK, Conti B, Volpe BT, Baker H (1999) Unique regulation of immediate early gene and tyrosine hydroxylase expression in the odor-deprived mouse olfactory bulb. J Biol Chem 274:3042–3047. doi:10.1074/jbc.274.5.3042
Mandairon N, Sacquet J, Garcia S, Ravel N, Jourdan F, Didier A (2006) Neurogenic correlates of an olfactory discrimination task in the adult olfactory bulb. Eur J NeuroSci 24:3578–3588. doi:10.1111/j.1460-9568.2006.05235.x
Min N, Joh TH, Corp ES, Baker H, Cubells JF, Son JH (1996) A transgenic mouse model to study transsynaptic regulation of tyrosine hydroxylase gene expression. J Neurochem 67:11–18
Nakashima A, Ota A, Sabban EL (2003) Interactions between Egr1 and AP1 factors in regulation of tyrosine hydroxylase transcription. Brain Res Mol Brain Res 112:61–69. doi:10.1016/S0169-328X(03)00047-0
Papanikolaou NA, Sabban EL (2000) Ability of Egr1 to activate tyrosine hydroxylase transcription in PC12 cells. Cross-talk with AP-1 factors. J Biol Chem 275:26683–26689
Sabban EL (1997) Control of tyrosine hydroxylase gene expression in chromaffin and PC12 cells. Semin Cell Dev Biol 8:101–111. doi:10.1006/scdb.1996.0129
Saino-Saito S, Sasaki H, Volpe BT, Kobayashi K, Berlin R, Baker H (2004) Differentiation of the dopaminergic phenotype in the olfactory system of neonatal and adult mice. J Comp Neurol 479:389–398. doi:10.1002/cne.20320
Saino-Saito S, Cave JW, Akiba Y, Sasaki H, Goto K, Kobayashi K, Berlin R, Baker H (2007) ER81 and CaMKIV identify anatomically and phenotypically defined subsets of mouse olfactory bulb interneurons. J Comp Neurol 502:485–496. doi:10.1002/cne.21293
Trocme C, Sarkis C, Hermel JM, Duchateau R, Harrison S, Simonneau M, Al-Shawi R, Mallet J (1998) CRE and TRE sequences of the rat tyrosine hydroxylase promoter are required for TH basal expression in adult mice but not in the embryo. Eur J NeuroSci 10:508–521. doi:10.1046/j.1460-9568.1998.00059.x
Vergano-Vera E, Yusta-Boyo MJ, de Castro F, Bernad A, de Pablo F, Vicario-Abejon C (2006) Generation of GABAergic and dopaminergic interneurons from endogenous embryonic olfactory bulb precursor cells. Development 133:4367–4379. doi:10.1242/dev.02601
Acknowledgments
This work was funded by NIH R01DC008955. Dr. Esther Sabban (New York Medical College) generously provided the EGR-1 antibody.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Akiba, N., Jo, S., Akiba, Y. et al. Expression of EGR-1 in a subset of olfactory bulb dopaminergic cells. J Mol Hist 40, 151–155 (2009). https://doi.org/10.1007/s10735-009-9217-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10735-009-9217-2