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Identification of two new genes from drought tolerant peanut up-regulated in response to drought

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Abstract

Using differential display of mRNA transcripts, we obtained nine partial cDNA clones that were up-regulated and down-regulated by exposure to drought. Two full length cDNAs of 825 bp and 700 bp, designated as Arachis hypogaea serine rich protein (AhSrp) and Arachis hypogaea leucine rich protein (AhLrp) were obtained using RLM-RACE. Based on the cDNA sequence, the transcriptional start site of AhSrp and AhLrp was assigned. AhSrp is predicted to code for a protein of 233 amino acids containing a signal peptide, three potential transmembrane domains and a sumolyation motif. AhLrp is predicted to code for a protein of 206 amino acids containing a signal peptide. The expression of partial cDNA and full-length cDNA were confirmed by RNA dot blot analysis. Expression of AhSrp was observed between 6 and 10 days of stress and prominent expression of AhLrp was visualized at 10 days of stress by semi quanititative PCR. Our studies have resulted in the identification of two new genes that may play a role in plant response to drought stress in peanut.

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Abbreviations

AhLrp :

Arachis hypogaea leucine rich protein

AhSrp :

Arachis hypogaea serine rich protein

cDNA:

Complementary DNA

DDRT-PCR:

Differential display reverse transcriptase PCR

DIG:

Digioxiginin

DT:

Drought tolerant variety K-1375

DS:

Drought susceptible variety Jl-24

PTDI:

Peanut transcript in drought induced

PTDS:

Peanut transcript drought suppressed

RLM RACE:

RNA ligase mediated rapid amplification of cDNA ends

RT-PCR:

Reverse transcriptase PCR

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Acknowledgements

We thank Prof. Gopinathan, Indian Institute of Science, Bangalore, India for providing facilities in his lab for all the DDRT-PCR experiments, Dr. K. S. S. Naik, Senior scientist, Acharya N. G. Ranga Agricultural University (ANGRAU) for providing the K-1375 and Jl-24 peanut varieties and USAID-Peanut CRSP Grant No. FAM # 51 for providing financial support to this work.

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Authors and Affiliations

  1. Department of Biotechnology, Jnanabharthi Campus, Bangalore University, Bangalore, 560056, India

    K. M. Devaiah, Geetha Bali & T. N. Athmaram

  2. Plant Biotechnology Division, Florida A&M University, Tallahassee, FL, USA

    M. S. Basha

Authors
  1. K. M. Devaiah
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  2. Geetha Bali
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  3. T. N. Athmaram
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  4. M. S. Basha
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Correspondence to K. M. Devaiah.

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K. M. Devaiah and Geetha Bali are equal contributors.

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Devaiah, K.M., Bali, G., Athmaram, T.N. et al. Identification of two new genes from drought tolerant peanut up-regulated in response to drought. Plant Growth Regul 52, 249–258 (2007). https://doi.org/10.1007/s10725-007-9195-x

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  • DOI: https://doi.org/10.1007/s10725-007-9195-x

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