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No direct binding of the heat-labile enterotoxin of Escherichia coli to E. coli lipopolysaccharides

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Abstract

A novel carbohydrate binding site recognizing blood group A and B determinants in a hybrid of cholera toxin and Escherichia coli heat-labile enterotoxin B-subunits (termed LCTBK) has previously been described, and also the native heat-labile enterotoxin bind to some extent to blood group A/B terminated glycoconjugates. The blood group antigen binding site is located at the interface of the B-subunits. Interestingly, the same area of the B-subunits has been proposed to be involved in binding of the heat-labile enterotoxin to lipopolysaccharides on the bacterial cell surface. Binding of the toxin to lipopolysaccharides does not affect the GM1 binding capacity. The present study aimed at characterizing the relationship between the blood group A/B antigen binding site and the lipopolysaccharide binding site. However, no binding of the B-subunits to E. coli lipopolysaccharides in microtiter wells or on thin-layer chromatograms was obtained. Incubation with lipopolysaccharides did not affect the binding of the B-subunits of heat-labile enterotoxin of human isolates to blood group A-carrying glycosphingolipids, indicating that the blood group antigen site is not involved in LPS binding. However, the saccharide competition experiments showed that GM1 binding reduced the affinity for blood group A determinants and vice versa, suggesting that a concurrent occupancy of the two binding sites does not occur. The latter finding is related to a connection between the blood group antigen binding site and the GM1 binding site through residues interacting with both ligands.

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Notes

  1. The abbreviations used are: CT, cholera toxin; CTB, cholera toxin B-subunits, ETEC, enterotoxigenic Escherichia coli; hLTB, heat-labile enterotoxin B-subunits from human isolates of E. coli; Kdo, 3-deoxy-D-manno-octulosonic acid; LPS; lipopolysaccharides; LT, heat-labile enterotoxin from E. coli.

    The glycosphingolipid nomenclature follows the recommendations by the IUPAC-IUB Commission on Biochemical Nomenclature (CBN for Lipids: Eur J Biochem (1998) 257, 293). It is assumed that Gal, Glc, GlcNAc, GalNAc and NeuAc are of the D-configuration, Fuc of the L-configuration, and all sugars are present in the pyranose form.

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Acknowledgements

This study was supported by the Swedish Medical Research Council (Grant No. 12628), the Swedish Cancer Foundation, and Magnus Bergvalls Foundation.

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Authors and Affiliations

  1. Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, P.O. Box 440, 405 30, Göteborg, Sweden

    Lena Jansson, Jonas Ångström & Susann Teneberg

  2. Institute of Biomedicine, Department of Medical Microbiology and Immunology, University of Gothenburg, P.O. Box 435, SE-40530, Göteborg, Sweden

    Michael Lebens

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  1. Lena Jansson
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Correspondence to Susann Teneberg.

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Jansson, L., Ångström, J., Lebens, M. et al. No direct binding of the heat-labile enterotoxin of Escherichia coli to E. coli lipopolysaccharides. Glycoconj J 27, 171–179 (2010). https://doi.org/10.1007/s10719-009-9264-7

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