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Genomic organization of repetitive DNAs in the cichlid fish Astronotus ocellatus

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Abstract

To contribute to the knowledge of fish genomes, we identified and characterized by means of nucleotide sequencing and physical chromosome mapping, three classes of repetitive DNAs in the genome of the South American cichlid fish Astronotus ocellatus. The first class corresponds to a satellite DNA family (AoSat) that shares similarity with a centromeric satellite DNA of the pufferfish Tetraodon nigroviridis. The second repetitive DNA class (AoRex3) is related to the retrotransposon Rex3, which is widely distributed among teleost fishes. The last repetitive element (AoLINE) shows a high similarity to the CR1-like LINE element of other teleosts. The three isolated repetitive elements are clustered in the centromeric heterochromatin of all chromosomes of the complement. The repetitive sequences are not randomly distributed in the genome, suggesting a pattern of compartmentalization on chromosomes.

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Acknowledgments

The authors are grateful to FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for financial support.

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  1. Departamento de Morfologia, Instituto de Biociências, UNESP—Universidade Estadual Paulista, CEP 18618-000, Botucatu, SP, Brazil

    Juliana Mazzuchelli & Cesar Martins

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  1. Juliana Mazzuchelli
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  2. Cesar Martins
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Correspondence to Cesar Martins.

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Mazzuchelli, J., Martins, C. Genomic organization of repetitive DNAs in the cichlid fish Astronotus ocellatus . Genetica 136, 461–469 (2009). https://doi.org/10.1007/s10709-008-9346-7

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