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Two distinct potato late blight resistance genes from Solanum berthaultii are located on chromosome 10

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Abstract

For breeding potato varieties resistant to late blight, identification of resistance genes to Phytophthora infestans (Rpi genes) is essential. Introduction of Rpi genes from wild Solanum species into cultivated potato is likely to be a good method to achieve durable resistance to P. infestans. In this study, we identified two Rpi genes (Rpi-ber1 and Rpi-ber2) derived from two different accessions of Solanum berthaultii. These two genes are closely linked on the long arm of chromosome 10. There are similarities between the predicted genetic locations of the previously identified Rpi-ber and Rpi-ber1, which given the common origin of these genes, may indicate that they are the same. However, the genetic positions of Rpi-ber1 and Rpi-ber2 are different. Rpi-ber1 is positioned between CT214 and TG63, whereas Rpi-ber2 is located below both of these two markers. In addition, the sequences of four linked markers to both R genes showed different polymorphisms indicating the two Rpi genes could be transmitted from different haplotypes (chromosomes).

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References

  • Bendahmane A, Kanyuke K, Baulcombe DC (1997) High-resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato. Theor Appl Genet 95:153–162

    Article  CAS  Google Scholar 

  • Bradshaw JE, Bryan GJ, Lees AK, McLean K, Solomon-Blackburn RM (2006) Mapping the R10 and R11 genes for resistance to late blight (Phytophthora infestans) present in the potato (Solanum tuberosum) R-gene differentials of Black. Theor Appl Genet 112:744–751

    Article  PubMed  CAS  Google Scholar 

  • Dangl JL, Jones JDG (2001) Plant pathogens and integrated defence responses to infection. Nature 411:826–833

    Article  PubMed  CAS  Google Scholar 

  • Day JP, Shattock RC (1997) Aggressiveness and other factors relating to displacement of populations of Phytophthora infestans in England and Wales. Eur J Plant Pathol 103:379–391

    Article  Google Scholar 

  • Dixon M, Jones DA, Keddie JS, Thomas CM, Harrison K, Jones JDG (1996) The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins. Cell 84:451–459

    Article  PubMed  CAS  Google Scholar 

  • Dixon M, Hatzixanthis K, Jones DA, Harrison K, Johns JDG (1998) The tomato Cf-5 disease resistance gene and six homologs show pronounced allelic variation in leucine-rich repeat copy number. Plant Cell 10:1915–1925

    Article  PubMed  CAS  Google Scholar 

  • El-Kharbotly A, Leonards-Schippers C, Huigen DJ, Jacobsen E, Pereira A, Stiekema WJ, Salamini F, Gebhardt C (1994) Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents. Mol Gen Genet 242:749–754

    Article  PubMed  CAS  Google Scholar 

  • El-Kharbotly A, Palomino-Sanchez C, Salamini F, Jacobsen E, Gebhardt C (1996) R6 and R7 alleles of potato conferring race-specific resistance to Phytophthora infestans (Mont.) de Bary identified genetic loci clustering with the R3 locus on chromosome XI. Theor Appl Genet 92:880–884

    Article  CAS  Google Scholar 

  • Ewing EE, Simko I, Smart CD, Bonierbale MW, Mizubuti ESG, May GD, Fry WE (2000) Genetic mapping from field of qualitative and quantitative resistance to Phytophthora infestans in a population derived from Solanum tuberosum and Solanum berthaultii. Mol Breed 6:25–36

    Article  CAS  Google Scholar 

  • Gebhardt C, Valkonen JPT (2001) Organization of genes controlling disease resistance in the potato genome. Annu Rev Phytopathol 39:79–102

    Article  PubMed  CAS  Google Scholar 

  • Goodwin SB, Sujkowski LS, Fry WE (1996) Widespread distribution and probable origin of resistance to metalaxyl in clonal genotypes of Phytophthora infestans in the United States and Western Canada. Phytopathology 86:793–800

    Article  CAS  Google Scholar 

  • Grube RC, Radwanski ER, Jahn M (2000) Comparative genetics of disease resistance within the Solanaceae. Genetics 155:873–887

    PubMed  CAS  Google Scholar 

  • Hawkes JG (1990) The potato: evolution, biodiversity and genetic resources. Belhaven Press, London

    Google Scholar 

  • Huang S (2005) The discovery and characterization of the major late blight resistance complex in potato: genomic structure, functional diversity and implications. PhD thesis, Wageningen University, Wageningen

  • Huang S, Vleeshouwers VGAA, Werij JS, Hutten RCB, van Eck HJ, Visser RGF, Jacobsen E (2004) The R3 resistance to Phytophthora infestans in potato is conferred by two closely linked R genes with distinct specificities. Mol Plant Microbe Interact 17:428–435

    Article  PubMed  CAS  Google Scholar 

  • Huang S, van der Vossen EAG, Kuang H, Vleeshouwers VGAA, Zhang N, Borm TJA, van Eck HJ, Baker B, Jacobsen E, Visser RGF (2005) Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. Plant J 42:251–261

    Article  PubMed  CAS  Google Scholar 

  • Hulbert SH, Webb CA, Smith SM, Sun Q (2001) Resistance gene complexes: evolution and utilization. Annu Rev Phytopathol 39:285–312

    Article  PubMed  CAS  Google Scholar 

  • Jansky S (2000) Breeding for disease resistance in potato. Plant Breed Rev 19:69–155

    Google Scholar 

  • Kuhl JC, Hanneman RE Jr, Havey MJ (2001) Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum. Mol Genet Genomics 265:977–985

    Article  PubMed  CAS  Google Scholar 

  • Leonards-Schippers C, Gieffers W, Salamini F, Gebhardt C (1992) The R1 gene conferring race-specific resistance to Phytophthora infestans in potato is located on potato chromosome V. Mol Gen Genet 233:278–283

    Article  PubMed  CAS  Google Scholar 

  • Li X, van Eck HJ, van der RouppeVoort JNAM, Huigen DJ, Stam P, Jacobsen E (1998) Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4. Theor Appl Genet 96:1121–1128

    Article  CAS  Google Scholar 

  • Malcolmson JF, Black W (1966) New R genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica 15:199–203

    Article  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Milligan SB, Bodeau J, Yaghoobi J, Kaloshian I, Zabel P, Williamson VM (1998) The root-knot nematode resistance gene Mi from tomato is a member of the leucien zipper nucleotide binding leucine-rich repeat family of plant genes. Plant Cell 10:1307–1319

    Article  PubMed  CAS  Google Scholar 

  • Naess SK, Bradeen JM, Wielgus SM, Haberlach GT, McGrath JM, Helgeson JP (2000) Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theor Appl Genet 101:697–704

    Article  CAS  Google Scholar 

  • Nombela G, Williamson VM, Muniz M (2003) The root-knot nematode resistance gene Mi-1.2 of tomato is responsible for resistance against the whitefly Bemisia tabaci. Mol Plant Microbe Interact 16:645–649

    Article  PubMed  CAS  Google Scholar 

  • Park T-H, Gros A, Sikkema A, Vleeshouwers VGAA, Muskens M, Allefs S, Jacobsen E, Visser RGF, van der Vossen EAG (2005a) The late blight resistance locus Rpi-blb3 from Solanum bulbocastanum belongs to a major late blight R gene cluster on chromosome 4 of potato. Mol Plant Microbe Interact 18:722–729

    Article  PubMed  CAS  Google Scholar 

  • Park T-H, Vleeshouwers VGAA, Hutten RCB, van Eck HJ, van der Vossen E, Jacobsen E, Visser RGF (2005b) High-resolution mapping and analysis of the resistance locus Rpi-abpt against Phytophthora infestans in potato. Mol Breed 16:33–43

    Article  CAS  Google Scholar 

  • Park T-H, Vleeshouwers VGAA, Huigen DJ, van der Vossen EAG, van Eck HJ, Visser RGF (2005c) Characterization and high-resolution mapping of a late blight resistance locus similar to R2 in potato. Theor Appl Genet 111:591–597

    Article  PubMed  CAS  Google Scholar 

  • Phumichai C, Ikeguchi-Samitsu Y, Fujimatsu M, Kitanishi S, Kobayashi A, Mori M, Hosaka K (2006) Expression of S-locus inhibitor gene (Sli) in various diploid potatoes. Euphytica 148:227–234

    Article  CAS  Google Scholar 

  • Rauscher GM, Smart CD, Simko I, Bonierbale M, Mayton H, Greenland A, Fry WE (2006) Characterization and mapping of Rpi-ber, a novel potato late blight resistance gene from Solanum berthaultii. Theor Appl Genet 112:674–687

    Article  PubMed  CAS  Google Scholar 

  • Rossi M, Goggin FL, Milligan SB, Kaloshian I, Ullman DE, Williamson VM (1998) The nematode resistance gene Mi of tomato confers resistance against the potato aphid. Proc Natl Acad Sci USA 95:9750–9754

    Article  PubMed  CAS  Google Scholar 

  • Rouppe Voort J, Kanyuka K, van der Vossen E, Bendahmane A, Mooijman P, Klein-Lankhorst R, Stiekema W, Baulcombe D, Bakker J (1999) Tight physical linkage of the nematode resistance gene Gpa2 and the virus resistance gene Rx on a single segment introgressed from the wild species Solanum tuberosum subsp. andigena CPC 1673 into cultivated potato. Mol Plant Microbe Interact 12:197–206

    Article  Google Scholar 

  • Schouten HJ, Krens FA, Jacobsen E (2006a) Cisgenic plants are similar to traditionally bred plants: international regulations for genetically modified organisms should be altered to exempt cisgenesis. EMBO Rep 7:750–753

    Article  PubMed  CAS  Google Scholar 

  • Schouten HJ, Krens FA, Jacobsen E (2006b) Do cisgenic plants warrant less stringent oversight? Nature Biotechnol 24:753

    Article  CAS  Google Scholar 

  • Śliwka J, Jakuczum H, Lebecka R, Marczewski W, Gebhardt C, Zimnoch-Guzowska E (2006) The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period. Theor Appl Genet 113:685–695

    Article  PubMed  Google Scholar 

  • Smilde WD, Brigneti G, Jagger L, Perkins S, Jones JD (2005) Solanum mochiquense chromosome IX carries a novel late blight resistance gene Rpi-moc1. Theor Appl Genet 110:252–258

    Article  PubMed  CAS  Google Scholar 

  • Świeżyński KM, Zimnoch-Guzowska E (2001) Breeding potato cultivars with tubers resistant to Phytophthora infestans. Potato Res 44:97–117

    Article  Google Scholar 

  • Turkensteen LJ (1993) Durable resistance of potatoes against Phytophthora infestans. In: Jacobs Th, Parlevliet JE (eds) Durability of disease resistance. Kluwer Academic Publisher, Dordrecht, pp 115–124

    Google Scholar 

  • Umaerus V, Umaerus M (1994) Inheritance of resistance to late blight. In: Bradshaw JE, Mackay GR (eds) Potato genetics. CAB International, Oxon, pp 365–401

    Google Scholar 

  • van der Vossen E, Sikkema A, Hekkert BL, Gros J, Stevens P, Muskens M, Wouters D, Pereira A, Stiekema W, Allefs S (2003) An ancient R gene from the wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato. Plant J 36:867–882

    Article  PubMed  Google Scholar 

  • van der Vossen EAG, Gros J, Sikkema A, Muskens M, Wouters D, Wolters P, Pereira A, Allefs S (2005) The Rpi-blb2 gene from Solanum bulbocastanum is an Mi-1 gene homolog conferring broad-spectrum late blight resistance in potato. Plant J 44:208–222

    Article  PubMed  Google Scholar 

  • Vleeshouwers VGAA, van Dooijeweert W, Paul Keizer LC, Sijpkes L, Govers F, Colon LT (1999) A laboratory assay for Phytophthora infestans resistance in various Solanum species reflects the field situation. Eur J Plant Pathol 105:241–250

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Vos P, Simons G, Jesse T, Wijbrandi J, Heinen L, Hogers R, Frijters A, Groenendijk J, Diergaarde P, Reijans M, Fierens-Onstenk J, de Both M, Peleman J, Liharska T, Hontelez J, Zabeau M (1998) The tomato Mi-1 gene confers resistance to both root-knot nematodes and potato aphids. Nature Biotechnol 16:1365–1369

    Article  CAS  Google Scholar 

  • Wastie RL (1991) Breeding for resistance. In: Ingram DS, Williams PH (eds) Advances in plant pathology 7: Phytophthora infestans the cause of late blight of potato. Academic Press, New York, pp 193–223

    Google Scholar 

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  1. Tae-Ho Park

    Present address: Brassica Genomics Team, National Institute of Agricultural Biotechnology, Suwon, 441-707, Republic of Korea

Authors and Affiliations

  1. The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK

    Tae-Ho Park, Simon Foster, Gianinna Brigneti & Jonathan D. G. Jones

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  1. Tae-Ho Park
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Correspondence to Jonathan D. G. Jones.

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Park, TH., Foster, S., Brigneti, G. et al. Two distinct potato late blight resistance genes from Solanum berthaultii are located on chromosome 10. Euphytica 165, 269–278 (2009). https://doi.org/10.1007/s10681-008-9784-4

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