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Population structure of Cylindrocladium parasiticum infecting peanuts (Arachis hypogaea) in Georgia, USA

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Abstract

Cylindrocladium parasiticum is an important pathogen of peanut (Arachis hypogaea) causing the disease Cylindrocladium black rot. The genetic structure of this haploid pathogen was determined for populations associated with peanut in Georgia, USA. Ten polymorphic microsatellite markers were used to determine genetic and genotypic diversity among three sub-populations representing the geographic distribution of peanut production in Georgia. Among 200 isolates collected, only 10 unique multilocus haplotypes were identified, with one multilocus haplotype occurring 176 times. Although no evidence for random mating was observed, the existence of loops in a constructed haplotype network hint at the possibility of recombination events. The absence of random mating might therefore be attributed to the homothallic nature of C. parasiticum as well as the clonality of the population, without excluding the possible occurrence of recombination. Cylindrocladium parasiticum thus appears to consist of a genetically homogeneous population throughout Georgia with mainly clonal reproduction or inbreeding contributing to the observed population genetic structure.

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Abbreviations

CBR:

Cylindrocladium black rot

MLH:

Multilocus haplotypes

IMH:

Indistinguishable multilocus haplotypes

DMH:

Distinguishable multilocus haplotypes

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Acknowledgements

We thank the National Research Foundation (NRF), members of the Tree Protection Co-operative Programme (TPCP) and the THRIP initiative of the Department of Trade and Industry (DTI), South Africa for financial support that made this study possible. We are also grateful to Almuth Hammerbacher for technical assistance.

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Author notes

  1. Louwrance P. Wright

    Present address: Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany

Authors and Affiliations

  1. Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany

    Andrew J. Davis

  2. Department of Genetics, Tree Protection Co-operative Programme, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa

    Louwrance P. Wright, Brenda D. Wingfield & Michael J. Wingfield

  3. Centraalbureau voor Schimmelcultures (CBS), P.O. Box 85167, 3508, Ad Utrecht, The Netherlands

    Pedro W. Crous

  4. Department of Plant Pathology, CPES, University of Georgia, Tifton, GA, 31794, USA

    Tim Brenneman

Authors
  1. Louwrance P. Wright
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  2. Andrew J. Davis
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  3. Brenda D. Wingfield
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  4. Pedro W. Crous
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  6. Michael J. Wingfield
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Correspondence to Louwrance P. Wright.

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Wright, L.P., Davis, A.J., Wingfield, B.D. et al. Population structure of Cylindrocladium parasiticum infecting peanuts (Arachis hypogaea) in Georgia, USA. Eur J Plant Pathol 127, 199–206 (2010). https://doi.org/10.1007/s10658-010-9584-2

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