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Visceral and Somatic Hypersensitivity in TNBS-Induced Colitis in Rats

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Abstract

Inflammation of visceral structures in rats has been shown to produce visceral/somatic hyperalgesia. Our objectives were to determine if trinitrobenzene sulfonic acid (TNBS) induced colitis in rats leads to visceral/somatic hypersensitivity. Male Sprague-Dawley rats (200–250 g) were treated with 20 mg of TNBS in 50% ethanol (n = 40) or an equivalent volume of ethanol (n = 40) or saline (n = 25) via the colon. Colonic distension, Von Frey, Hargreaves, and tail reflex tests were used to evaluate for visceral, mechanical, and thermal sensitivity. The rats demonstrated visceral hypersensitivity at 2–28 days following TNBS administration (P < 0.0001). The ethanol-treated rats also demonstrated visceral hypersensitivity that resolved after day 14. TNBS-treated rats demonstrated somatic hypersensitivity at days 14–28 (P < 0.0001) in response to somatic stimuli of the hind paw. TNBS colitis is associated with visceral and somatic hypersensitivity in areas of somatotopic overlap. This model of colitis should allow further investigation into the mechanisms of visceral and somatic hypersensitivity.

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Acknowledgements

G.N.V. is supported by a Merit Review Award (PI: GN Verne) from the Medical Research Service of the Department of Veteran Affairs and NIH Grant 1-R01-NS053090–01 (PI: GN Verne).

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Authors and Affiliations

  1. Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Florida College of Medicine, 1600 SW Archer Road, HD 602, Gainesville, FL, 32610-0214, USA

    QiQi Zhou & G. Nicholas Verne

  2. Department of Oral and Maxillofacial Surgery, University of Florida Colleges of Medicine and Dentistry, Gainesville, FL, USA

    Donald D. Price & Robert M. Caudle

  3. Department of Neuroscience, University of Florida Colleges of Medicine and Dentistry, Gainesville, FL, USA

    Donald D. Price & Robert M. Caudle

  4. North Florida/South Georgia VA Medical System, Gainesville, FL, USA

    G. Nicholas Verne

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  1. QiQi Zhou
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Correspondence to G. Nicholas Verne.

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Zhou, Q., Price, D.D., Caudle, R.M. et al. Visceral and Somatic Hypersensitivity in TNBS-Induced Colitis in Rats. Dig Dis Sci 53, 429–435 (2008). https://doi.org/10.1007/s10620-007-9881-6

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  • DOI: https://doi.org/10.1007/s10620-007-9881-6

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