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Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)

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Abstract

High quality jumping microsatellite libraries of the plateau pika (Ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed accordingly. Polymorphism was assessed for 48 individuals. Only 13 microsatellite loci were polymorphic with high polymorphism information content (PIC) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment being very closely related. This degree of polymorphism, however, is sufficient to conduct parentage analysis. Expected and observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. One locus was in linkage disequilibrium. This information provides efficient tools to allow future parentage studies.

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Acknowledgments

We would like to thank Professor A. T. Smith for his comments and careful editing of this manuscript. We thank Jin Yang, Wenjing Li, Jiapeng Qu for their help. This work was supported by the National Science Foundation of China (No. 30670357), Key Innovation Research Programs of the Chinese Academy of Sciences (No. KSCX2-YW-N-06), The Western Light Projects of China, and the grants from “973” Project of China Ministry of Science and Technology (No. 2007CB109100).

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Authors and Affiliations

  1. Graduate School of the Chinese Academy of Sciences, Beijing, China

    Ke-xin Li & Jia-ning Geng

  2. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, No. 59 XiGuan Avenue, Xining, Qinghai, 810001, China

    Ke-xin Li & Yan-ming Zhang

  3. Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, The Chinese Academy of Sciences, No. 7 Beitucheng West Road, ChaoYang, Beijing, 100029, China

    Jia-ning Geng, Jin Yang & Song-nian Hu

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  1. Ke-xin Li
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  2. Jia-ning Geng
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  3. Jin Yang
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  4. Yan-ming Zhang
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  5. Song-nian Hu
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Corresponding authors

Correspondence to Yan-ming Zhang or Song-nian Hu.

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Ke-xin Li and Jia-ning Geng contributed equally to this paper.

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Li, Kx., Geng, Jn., Yang, J. et al. Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae). Conserv Genet 10, 785–787 (2009). https://doi.org/10.1007/s10592-008-9662-6

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  • DOI: https://doi.org/10.1007/s10592-008-9662-6

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