Abstract
We describe 9 primers for amplification of microsatellite loci for the Neotropical tree Cedrela odorata L. (Meliaceae). Loci were isolated from an enriched library derived from a single DNA sample from a tree in Costa Rica. Levels of polymorphism were determined using samples from a large progeny trial. Across loci, the number of alleles ranged from 14 to 30. Observed heterozygosity levels ranged from 0.61 to 0.88. No linkage disequilibria were detected although some departures from Hardy-Weinberg equilibrium (HWE) were found, probably due to a Wahlund effect.
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Acknowledgements
This work was part of the project SEEDSOURCE, funded by the EC under FP6 INCO (contract 003708). Library development, cloning and sequencing was done by CNR and optimisation at CEH by GH during his M.Sc., sponsored by CATIE and Ministerio de Ciencia y Tecnología.
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Hernández, G., Buonamici, A., Walker, K. et al. Isolation and characterization of microsatellite markers for Cedrela odorata L. (Meliaceae), a high value neotropical tree. Conserv Genet 9, 457–459 (2008). https://doi.org/10.1007/s10592-007-9334-y
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DOI: https://doi.org/10.1007/s10592-007-9334-y