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Study of a novel cell lysis method with titanium dioxide for Lab-on-a-Chip devices

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Abstract

In this paper, a novel method is proposed and demonstrated to be able to lyse gram-negative (E. coli) bacteria cells for Lab-on-a-Chip applications. The proposed method incorporates using titanium dioxide particles as photocatalysts and a miniaturized UV LED array as an excitation light source to perform cell lysis on microchips. The experimental result demonstrates the feasibility of the proposed prototype device. The working device suggests an inexpensive, easy to be fabricated and effective way for microchip cell lysis. The miniaturized UV LED array and the microchip with a reaction chamber can be easily integrated with other functional components to form a customized whole Lab-on-a-Chip system.

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Acknowledgments

The authors thank the National Sciences and Engineering Research Council of Canada (NSERC) and the University of Waterloo for financial support and Professor Perry Chou in department of Chemical Engineering at the University of Waterloo for his generous support and valuable suggestions.

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Authors and Affiliations

  1. Department of Systems Design Engineering, University of Waterloo, Waterloo, Canada, N2L 3G1

    Weijie Wan & John T. W. Yeow

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  1. Weijie Wan
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  2. John T. W. Yeow
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Correspondence to John T. W. Yeow.

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Wan, W., Yeow, J.T.W. Study of a novel cell lysis method with titanium dioxide for Lab-on-a-Chip devices. Biomed Microdevices 13, 527–532 (2011). https://doi.org/10.1007/s10544-011-9521-y

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  • DOI: https://doi.org/10.1007/s10544-011-9521-y

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