Abstract
Arabidopsis thaliana plants (wild type accessions Col and N1438) were grown in nutrient solution for 34 d with or without 50 mM NaCl. Salt stress inhibited plant growth rate more in Col than in N1438 and a decrease in K+, Ca2+ and nitrogen contents was observed in both accessions. NaCl diminished accumulation of malate, fumarate and citrate only in Col accession. To measure the effect of NaCl on transcript level of superoxide dismutase (SOD) isoforms and copper chaperone for SOD genes, a semi-quantitative polymerase chain reaction (RT-PCR) method was developed using cDNA normalized against the EF1a gene in parallel with quantitative real time RT-PCR (Q-PCR) technique. Both methods gave the same results. The abundance of transcripts of the three genes coding for Cu/Zn-SOD responded similarly to NaCl in both accessions: CSD1 gene was overexpressed, and CSD2 and CSD3 genes were repressed. However, the genes coding for Fe-SOD (FSD1), Mn-SOD (MSD1) and Cu-chaperone for SOD (CCS) responded to NaCl differently in Col and N1438: the former gene was overexpressed in Col and repressed in N1438, and the opposite behaviour was observed for the latter two genes.
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Abbreviations
- CCS:
-
copper chaperone for SOD
- Q-PCR:
-
quantitative real time RT-PCR
- ROS:
-
reactive oxygen species
- RT-PCR:
-
reverse transcription polymerase chain reaction
- SOD:
-
superoxide dismutase
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Acknowledgements
This work was supported by the Tunisian-French Comité Mixte de Coopération Universitaire (CMCU network 02F/924). We are indebted to Prof. J.-C. Davidian, Dr. F. Cellier, Dr. P. Berthomieu, and Dr. L. Lejay for their help, and we thank Prof. C. Grignon for valuable discussion of the results. H.A. is grateful to the Tunisian government for a financial support to her stay in France, provided through a doctoral grant. She specially thanks Dr. A. Gojon for having welcomed her in their research groups.
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Attia, H., Karray, N., Msilini, N. et al. Effect of salt stress on gene expression of superoxide dismutases and copper chaperone in Arabidopsis thaliana . Biol Plant 55, 159–163 (2011). https://doi.org/10.1007/s10535-011-0022-x
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DOI: https://doi.org/10.1007/s10535-011-0022-x